Spodoptera litura (Asian cotton leafworm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia;

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U7BG86|A0A4U7BG86_SPOLT Cuticular protein CPAP3-A1 OS=Spodoptera litura OX=69820 GN=BJG85_SLAki020 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.58SFIVLALFVAAAVAAPASPDD22 pKa = 2.95ADD24 pKa = 3.34AVVVRR29 pKa = 11.84YY30 pKa = 10.17DD31 pKa = 3.31SDD33 pKa = 4.15NIGVDD38 pKa = 3.95GYY40 pKa = 11.1NYY42 pKa = 10.75AVEE45 pKa = 4.22TSNGIAAQEE54 pKa = 3.79QGQLKK59 pKa = 10.27NAGTEE64 pKa = 3.9NEE66 pKa = 4.25AIEE69 pKa = 4.21VRR71 pKa = 11.84GQFSYY76 pKa = 10.22TGPDD80 pKa = 3.04GVVYY84 pKa = 9.37TVTYY88 pKa = 9.49IANEE92 pKa = 3.97QGFQPQGAHH101 pKa = 6.72IPQAPP106 pKa = 3.19

Molecular weight: 11.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V6DVF6|A0A4V6DVF6_SPOLT Cuticular protein CPG OS=Spodoptera litura OX=69820 GN=BJG85_SLAki221 PE=4 SV=1
MM1 pKa = 7.33SRR3 pKa = 11.84LTIFFVIAVAYY14 pKa = 8.9EE15 pKa = 4.03VIADD19 pKa = 3.99DD20 pKa = 5.2SRR22 pKa = 11.84AEE24 pKa = 3.75QSGFISPVIAAGNLGLAGAAIAGGPGIIGGPAVVAAGPAIVGAGPAVVASGPAVVAGGPVIGSGAALNNAAAVGAAQLSAIQNAQAVQAAQIANSAAAAIQEE126 pKa = 4.05ARR128 pKa = 11.84ATEE131 pKa = 3.84AAARR135 pKa = 11.84AGQAQAMNNAAALNAARR152 pKa = 11.84VANIQRR158 pKa = 11.84AQAAAWEE165 pKa = 4.23NARR168 pKa = 11.84ATEE171 pKa = 3.84AARR174 pKa = 11.84RR175 pKa = 11.84AGAGAALEE183 pKa = 4.04AARR186 pKa = 11.84AAEE189 pKa = 4.12AARR192 pKa = 11.84VADD195 pKa = 4.07AARR198 pKa = 11.84LQAIAIANTRR208 pKa = 11.84NVEE211 pKa = 3.85AARR214 pKa = 11.84IANAARR220 pKa = 11.84AQAAAVANSAAQAQAVADD238 pKa = 4.29TVARR242 pKa = 11.84TAHH245 pKa = 6.91DD246 pKa = 3.86GALLLGGGGALVGGGAVLAGPAVAVGGLGGYY277 pKa = 10.02GLGGAGKK284 pKa = 9.91LIHH287 pKa = 6.59HH288 pKa = 7.47

Molecular weight: 27.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

283

0

283

62312

68

1312

220.2

23.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.193 ± 0.565

0.741 ± 0.103

4.68 ± 0.175

4.917 ± 0.195

2.688 ± 0.091

8.059 ± 0.491

5.265 ± 0.405

3.731 ± 0.14

4.701 ± 0.188

4.84 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.875 ± 0.063

3.593 ± 0.245

8.379 ± 0.293

4.585 ± 0.197

3.37 ± 0.219

7.514 ± 0.267

4.383 ± 0.303

8.843 ± 0.323

0.406 ± 0.049

6.236 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski