Laetiporus sulphureus 93-53

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Laetiporaceae; Laetiporus; Laetiporus sulphureus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13712 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A165HBS7|A0A165HBS7_9APHY Telomere_reg-2 domain-containing protein OS=Laetiporus sulphureus 93-53 OX=1314785 GN=LAESUDRAFT_740990 PE=3 SV=1
MM1 pKa = 7.47TEE3 pKa = 4.25STSQSPSLLAMARR16 pKa = 11.84NKK18 pKa = 10.4LLSTSVNGVKK28 pKa = 10.35DD29 pKa = 3.48SSSLHH34 pKa = 5.25RR35 pKa = 11.84WVLLRR40 pKa = 11.84NSLVQPHH47 pKa = 6.19TPAPIATPEE56 pKa = 4.23KK57 pKa = 10.2ADD59 pKa = 3.15VPYY62 pKa = 10.6VYY64 pKa = 10.28RR65 pKa = 11.84RR66 pKa = 11.84VEE68 pKa = 5.22DD69 pKa = 3.66EE70 pKa = 4.46GCDD73 pKa = 3.57QEE75 pKa = 5.2EE76 pKa = 4.16EE77 pKa = 4.62VAFMFPDD84 pKa = 4.2PDD86 pKa = 3.79ALSRR90 pKa = 11.84DD91 pKa = 3.48AGVCGSEE98 pKa = 4.2NDD100 pKa = 3.63WLDD103 pKa = 3.93SLLEE107 pKa = 4.34DD108 pKa = 5.26LGEE111 pKa = 5.62DD112 pKa = 3.76DD113 pKa = 6.44DD114 pKa = 6.58LDD116 pKa = 4.93AEE118 pKa = 4.4EE119 pKa = 5.6GGLEE123 pKa = 4.17TSMSGAPPDD132 pKa = 4.41EE133 pKa = 5.26DD134 pKa = 5.72DD135 pKa = 4.67EE136 pKa = 4.73PLSPLYY142 pKa = 11.11SPMSSSDD149 pKa = 3.42NLVDD153 pKa = 3.6QSSFYY158 pKa = 10.91VNPHH162 pKa = 7.54AIPIPYY168 pKa = 8.47PIPYY172 pKa = 9.42PPVRR176 pKa = 11.84PSLVSAWYY184 pKa = 10.24GLDD187 pKa = 3.46SSPDD191 pKa = 3.54SLLEE195 pKa = 4.04ASSPLYY201 pKa = 10.36HH202 pKa = 7.69DD203 pKa = 4.54PLPYY207 pKa = 10.42YY208 pKa = 10.67DD209 pKa = 4.92VDD211 pKa = 3.85DD212 pKa = 4.78TEE214 pKa = 5.15DD215 pKa = 4.25LPVPDD220 pKa = 5.83AIEE223 pKa = 4.42DD224 pKa = 3.94TSDD227 pKa = 4.05DD228 pKa = 4.06EE229 pKa = 5.53SDD231 pKa = 3.98APATPFDD238 pKa = 4.23HH239 pKa = 6.4STSSICPLDD248 pKa = 4.09PASVPLPSEE257 pKa = 3.81RR258 pKa = 11.84RR259 pKa = 11.84RR260 pKa = 11.84RR261 pKa = 11.84SHH263 pKa = 6.26SRR265 pKa = 11.84QPQVYY270 pKa = 9.36VDD272 pKa = 3.6TDD274 pKa = 3.16DD275 pKa = 4.54SYY277 pKa = 11.82FYY279 pKa = 10.67PFGLDD284 pKa = 3.46PLPSHH289 pKa = 7.32VDD291 pKa = 3.58NPVHH295 pKa = 6.8AARR298 pKa = 11.84VYY300 pKa = 10.49GPSVYY305 pKa = 9.37QQCC308 pKa = 3.84

Molecular weight: 33.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165DLL6|A0A165DLL6_9APHY Clavaminate synthase-like protein OS=Laetiporus sulphureus 93-53 OX=1314785 GN=LAESUDRAFT_715172 PE=4 SV=1
MM1 pKa = 7.8SPFLLSALFILHH13 pKa = 6.96LPHH16 pKa = 6.89SARR19 pKa = 11.84LSRR22 pKa = 11.84FRR24 pKa = 11.84SRR26 pKa = 11.84TSSPQSALSSHH37 pKa = 6.85LPAQARR43 pKa = 11.84PPLAKK48 pKa = 9.92RR49 pKa = 11.84RR50 pKa = 11.84ALPVSQTPP58 pKa = 3.21

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13707

5

13712

5380101

49

5037

392.4

43.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.944 ± 0.019

1.379 ± 0.01

5.534 ± 0.014

6.144 ± 0.022

3.545 ± 0.012

6.226 ± 0.019

2.706 ± 0.011

4.78 ± 0.015

4.328 ± 0.02

9.23 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.007

3.174 ± 0.01

6.287 ± 0.027

3.74 ± 0.015

6.553 ± 0.021

8.666 ± 0.028

5.857 ± 0.016

6.483 ± 0.014

1.438 ± 0.009

2.688 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski