Vulcanisaeta sp. SCGC AB-777_J10

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae; Vulcanisaeta; unclassified Vulcanisaeta

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 918 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T9WWQ0|A0A2T9WWQ0_9CREN Uncharacterized protein OS=Vulcanisaeta sp. SCGC AB-777_J10 OX=1987493 GN=DDW08_02560 PE=4 SV=1
MM1 pKa = 7.36STPQTQWIILSMVFLVLAAVFGTLWFITNQNYY33 pKa = 6.81TTLLASYY40 pKa = 7.77NTLKK44 pKa = 10.8AQYY47 pKa = 10.82DD48 pKa = 3.85NLQTQCNTLQAEE60 pKa = 4.53LNNLQSQYY68 pKa = 11.17SSCEE72 pKa = 3.83TQLNNLQAQNTQLKK86 pKa = 9.98QEE88 pKa = 4.14LQGCGLTTTLQVQAVGLTGVSSGSSANLTLLISNPSSSGIGLNGFTLGSLSCVFSSPVQVPAGTQGGEE156 pKa = 3.96LVIRR160 pKa = 11.84LAMSNGAFDD169 pKa = 4.33GVSDD173 pKa = 4.0IYY175 pKa = 11.4GNGIIVPGGIAASCSGSQTAAVGIQYY201 pKa = 10.22SGYY204 pKa = 7.41ITTVSGQTYY213 pKa = 8.92PFTVTASSS221 pKa = 3.2

Molecular weight: 23.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T9WU59|A0A2T9WU59_9CREN Uncharacterized protein OS=Vulcanisaeta sp. SCGC AB-777_J10 OX=1987493 GN=DDW08_04020 PE=4 SV=1
MM1 pKa = 6.9TAYY4 pKa = 10.49ALRR7 pKa = 11.84RR8 pKa = 11.84PDD10 pKa = 3.15VSGTFLEE17 pKa = 4.25RR18 pKa = 11.84LNRR21 pKa = 11.84FVGIAEE27 pKa = 4.09INGEE31 pKa = 4.03RR32 pKa = 11.84VRR34 pKa = 11.84VHH36 pKa = 5.27IHH38 pKa = 5.99DD39 pKa = 4.52PGRR42 pKa = 11.84LRR44 pKa = 11.84EE45 pKa = 4.03LLRR48 pKa = 11.84PGVRR52 pKa = 11.84IHH54 pKa = 7.49AYY56 pKa = 9.97NKK58 pKa = 9.35VGGKK62 pKa = 6.55TRR64 pKa = 11.84YY65 pKa = 9.69YY66 pKa = 10.72LVAVDD71 pKa = 5.61LGDD74 pKa = 3.68EE75 pKa = 4.18LVLVNSAIHH84 pKa = 6.15NDD86 pKa = 2.69IVAWLVSNGIVLRR99 pKa = 11.84RR100 pKa = 11.84YY101 pKa = 8.54QVVRR105 pKa = 11.84RR106 pKa = 11.84EE107 pKa = 4.05PVFGSGRR114 pKa = 11.84FDD116 pKa = 3.83LLLRR120 pKa = 11.84SPSGKK125 pKa = 9.29EE126 pKa = 3.22AFMEE130 pKa = 4.52VKK132 pKa = 10.48GVTLVDD138 pKa = 3.19NGVAKK143 pKa = 10.66FPDD146 pKa = 4.45PYY148 pKa = 10.67GQASFLQGRR157 pKa = 11.84GKK159 pKa = 10.4VFKK162 pKa = 10.74LLFATSVVCLLLNRR176 pKa = 11.84LGLGRR181 pKa = 11.84ILRR184 pKa = 11.84RR185 pKa = 11.84SS186 pKa = 3.27

Molecular weight: 20.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

918

0

918

254953

35

1192

277.7

31.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.016 ± 0.084

0.733 ± 0.034

4.987 ± 0.071

6.322 ± 0.091

3.468 ± 0.052

7.549 ± 0.073

1.489 ± 0.032

8.081 ± 0.08

4.91 ± 0.086

10.454 ± 0.103

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.582 ± 0.036

4.145 ± 0.07

4.478 ± 0.068

1.879 ± 0.041

6.545 ± 0.097

5.771 ± 0.074

4.895 ± 0.085

8.986 ± 0.074

1.285 ± 0.037

4.424 ± 0.075
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski