Amsacta moorei entomopoxvirus (AmEPV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Entomopoxvirinae; Betaentomopoxvirus

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 278 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9EMU7|Q9EMU7_AMEPV AMV102 OS=Amsacta moorei entomopoxvirus OX=28321 GN=AMV102 PE=4 SV=1
MM1 pKa = 7.53SNADD5 pKa = 3.24YY6 pKa = 11.18VIIYY10 pKa = 10.28NCGNDD15 pKa = 2.98IEE17 pKa = 4.75SYY19 pKa = 11.19KK20 pKa = 11.03DD21 pKa = 3.01IDD23 pKa = 4.05LSCDD27 pKa = 3.25VNIPITNDD35 pKa = 2.85DD36 pKa = 4.39MIISDD41 pKa = 4.97EE42 pKa = 3.99ILSYY46 pKa = 10.72EE47 pKa = 3.91SSIYY51 pKa = 10.33IITNYY56 pKa = 10.84DD57 pKa = 3.32DD58 pKa = 6.19DD59 pKa = 4.08EE60 pKa = 6.64CNDD63 pKa = 3.55QDD65 pKa = 4.86CDD67 pKa = 5.49LILISNDD74 pKa = 3.31QIDD77 pKa = 5.32KK78 pKa = 10.79IDD80 pKa = 3.57LCKK83 pKa = 10.51LKK85 pKa = 10.9KK86 pKa = 10.37FINNKK91 pKa = 9.21SEE93 pKa = 4.06IIIVSKK99 pKa = 11.48NEE101 pKa = 3.69DD102 pKa = 3.32FQNNIKK108 pKa = 10.36EE109 pKa = 4.46IITDD113 pKa = 3.67ADD115 pKa = 3.93VTCYY119 pKa = 10.72TEE121 pKa = 3.9FKK123 pKa = 10.55YY124 pKa = 10.59IIGITNCLLNIKK136 pKa = 8.68CLKK139 pKa = 9.52KK140 pKa = 9.92IYY142 pKa = 9.64RR143 pKa = 11.84IKK145 pKa = 10.72VFNYY149 pKa = 10.46DD150 pKa = 3.46NLPP153 pKa = 3.54

Molecular weight: 17.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9EMP6|Q9EMP6_AMEPV Serine/threonine-protein kinase OS=Amsacta moorei entomopoxvirus OX=28321 GN=AMV153 PE=3 SV=1
MM1 pKa = 7.9YY2 pKa = 9.18MIYY5 pKa = 10.29ILISHH10 pKa = 6.92IWFHH14 pKa = 5.55FKK16 pKa = 9.47IWFKK20 pKa = 10.6IICFSYY26 pKa = 9.9ICNIINNFIFKK37 pKa = 10.1FINFFYY43 pKa = 10.35IFKK46 pKa = 10.61VFFNVRR52 pKa = 11.84KK53 pKa = 9.91RR54 pKa = 11.84KK55 pKa = 9.43IINNIIYY62 pKa = 10.63NFTTT66 pKa = 3.25

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

278

0

278

71083

60

1384

255.7

30.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.533 ± 0.083

1.872 ± 0.098

6.011 ± 0.119

4.889 ± 0.115

5.024 ± 0.124

2.196 ± 0.1

1.355 ± 0.055

15.251 ± 0.273

9.836 ± 0.171

9.189 ± 0.164

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.822 ± 0.065

13.027 ± 0.246

2.03 ± 0.071

1.76 ± 0.076

2.112 ± 0.09

6.257 ± 0.186

4.313 ± 0.152

3.457 ± 0.102

0.494 ± 0.04

7.571 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski