Hydrogenobaculum sp. (strain Y04AAS1)

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Aquificaceae; Hydrogenobaculum; unclassified Hydrogenobaculum

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1626 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B4U6Z8|ILVD_HYDS0 Dihydroxy-acid dehydratase OS=Hydrogenobaculum sp. (strain Y04AAS1) OX=380749 GN=ilvD PE=3 SV=1
MM1 pKa = 7.08FRR3 pKa = 11.84AMWTSAAGMTAQQQNLDD20 pKa = 3.92VISNNMANVDD30 pKa = 3.75TVGYY34 pKa = 9.64KK35 pKa = 9.77QLQPVFDD42 pKa = 4.36DD43 pKa = 6.2LIYY46 pKa = 10.05QTVKK50 pKa = 10.87DD51 pKa = 4.19PGMEE55 pKa = 3.99TSQNSQNPIGYY66 pKa = 8.92QLGLGAYY73 pKa = 9.2IDD75 pKa = 3.67GTYY78 pKa = 10.96GIFTQGNVQQTGNPLDD94 pKa = 3.73VAIQGNGFFQVAMPDD109 pKa = 3.19GTIAYY114 pKa = 6.8TRR116 pKa = 11.84NGQLQLDD123 pKa = 4.11ANGDD127 pKa = 3.95LVTADD132 pKa = 4.35GYY134 pKa = 11.17LIQPQITVPPNATSVNIGPDD154 pKa = 3.04GTVAVTIPGQQGVQTVGQLQMANFVNPAGLKK185 pKa = 10.33RR186 pKa = 11.84IGNNLFEE193 pKa = 4.27QTQASGNPIVSNPGTQGLGTLLSGYY218 pKa = 8.62TEE220 pKa = 4.21ASNVNIVNEE229 pKa = 4.11MVNLIIAEE237 pKa = 3.95NAYY240 pKa = 10.04QFNSKK245 pKa = 10.58GITAANTMLSTAANLYY261 pKa = 9.44TGG263 pKa = 4.05

Molecular weight: 27.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4U720|B4U720_HYDS0 Uncharacterized protein OS=Hydrogenobaculum sp. (strain Y04AAS1) OX=380749 GN=HY04AAS1_0239 PE=4 SV=1
MM1 pKa = 7.62AVPKK5 pKa = 10.33RR6 pKa = 11.84RR7 pKa = 11.84TSRR10 pKa = 11.84WRR12 pKa = 11.84RR13 pKa = 11.84NQRR16 pKa = 11.84RR17 pKa = 11.84AQAFFAKK24 pKa = 10.15LKK26 pKa = 9.64SFNMVKK32 pKa = 10.32CPNCGEE38 pKa = 3.97FAMPHH43 pKa = 6.23RR44 pKa = 11.84ACPYY48 pKa = 8.75CGYY51 pKa = 10.7YY52 pKa = 9.5RR53 pKa = 11.84DD54 pKa = 3.91KK55 pKa = 11.16QVLEE59 pKa = 4.27VV60 pKa = 3.53

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1626

0

1626

492277

30

1563

302.8

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.153 ± 0.054

0.861 ± 0.023

5.388 ± 0.046

6.991 ± 0.089

5.196 ± 0.059

6.048 ± 0.056

1.702 ± 0.022

9.318 ± 0.054

9.211 ± 0.073

9.678 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.026

5.04 ± 0.056

3.648 ± 0.043

2.556 ± 0.039

3.364 ± 0.039

6.246 ± 0.054

4.531 ± 0.039

6.412 ± 0.049

0.766 ± 0.019

4.556 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski