Picobirnavirus sp.

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Picobirnaviridae; Picobirnavirus; unclassified Picobirnavirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291S0D7|A0A291S0D7_9VIRU Uncharacterized protein OS=Picobirnavirus sp. OX=1907787 PE=4 SV=1
MM1 pKa = 7.21NKK3 pKa = 8.34KK4 pKa = 8.56TGRR7 pKa = 11.84NQIPKK12 pKa = 9.74SNKK15 pKa = 9.33AKK17 pKa = 10.72ASTTGRR23 pKa = 11.84KK24 pKa = 8.66GRR26 pKa = 11.84GMRR29 pKa = 11.84DD30 pKa = 3.15QHH32 pKa = 6.1SQRR35 pKa = 11.84SDD37 pKa = 2.93NRR39 pKa = 11.84NVNSSGNYY47 pKa = 9.84NGDD50 pKa = 2.8IDD52 pKa = 5.7APATNNPDD60 pKa = 2.93WWNKK64 pKa = 9.19SATFADD70 pKa = 4.2SVRR73 pKa = 11.84IPFNRR78 pKa = 11.84ITGYY82 pKa = 9.86PISKK86 pKa = 10.23NDD88 pKa = 3.85LLPRR92 pKa = 11.84SIFQDD97 pKa = 3.28TTIVADD103 pKa = 3.85EE104 pKa = 4.86SYY106 pKa = 10.43PEE108 pKa = 5.2AIAIPNLMCIHH119 pKa = 6.82YY120 pKa = 10.36IPFTGACSNWNDD132 pKa = 4.38PINRR136 pKa = 11.84SYY138 pKa = 10.89MSVYY142 pKa = 10.37SALAAKK148 pKa = 9.68TSGAMQINQPDD159 pKa = 3.56LAMTTEE165 pKa = 4.28AANSVIVLIYY175 pKa = 9.37EE176 pKa = 4.55LKK178 pKa = 10.3RR179 pKa = 11.84ALGIANLYY187 pKa = 8.79SQQNKK192 pKa = 7.22TLPYY196 pKa = 9.78HH197 pKa = 6.32VLQALHH203 pKa = 7.21IDD205 pKa = 3.57PDD207 pKa = 4.68SIIGHH212 pKa = 5.31QDD214 pKa = 3.1EE215 pKa = 4.5IRR217 pKa = 11.84RR218 pKa = 11.84EE219 pKa = 3.76LNYY222 pKa = 11.0YY223 pKa = 9.91IGALNSLALPAYY235 pKa = 8.68TDD237 pKa = 2.74IHH239 pKa = 6.48ARR241 pKa = 11.84RR242 pKa = 11.84AQLAGNVYY250 pKa = 10.79ADD252 pKa = 3.96EE253 pKa = 5.39DD254 pKa = 3.93DD255 pKa = 3.77VRR257 pKa = 11.84AAFYY261 pKa = 10.39AYY263 pKa = 8.47VTAGYY268 pKa = 9.98YY269 pKa = 10.31EE270 pKa = 4.43YY271 pKa = 11.27VDD273 pKa = 3.72TADD276 pKa = 5.11DD277 pKa = 3.71ISDD280 pKa = 3.79VEE282 pKa = 4.24HH283 pKa = 6.84LKK285 pKa = 10.34FVYY288 pKa = 9.2MAASTPRR295 pKa = 11.84TADD298 pKa = 2.98SYY300 pKa = 10.69LTAIGDD306 pKa = 4.34CINAIRR312 pKa = 11.84NSSAFGLISAAVQRR326 pKa = 11.84GFGDD330 pKa = 3.61RR331 pKa = 11.84NLLTVEE337 pKa = 4.14MVPVDD342 pKa = 4.05FVVIPAVDD350 pKa = 3.34RR351 pKa = 11.84YY352 pKa = 7.66MTYY355 pKa = 8.53QTQNADD361 pKa = 4.21FIGHH365 pKa = 6.1YY366 pKa = 10.38NDD368 pKa = 5.16LDD370 pKa = 4.02SKK372 pKa = 11.04SLHH375 pKa = 5.87IVQNPVRR382 pKa = 11.84NALEE386 pKa = 4.16VIPTIKK392 pKa = 10.4SNSSHH397 pKa = 6.56YY398 pKa = 9.59PEE400 pKa = 4.11NWACIVNDD408 pKa = 3.85NKK410 pKa = 10.27WLNSYY415 pKa = 11.04DD416 pKa = 4.22GITDD420 pKa = 3.48ADD422 pKa = 3.95FVIEE426 pKa = 4.01ATRR429 pKa = 11.84LKK431 pKa = 10.84VATTAIEE438 pKa = 4.4DD439 pKa = 3.64PGFEE443 pKa = 4.15YY444 pKa = 10.98GMDD447 pKa = 3.24IPLDD451 pKa = 3.59VTGTEE456 pKa = 3.57ICLNFHH462 pKa = 5.42IWKK465 pKa = 10.18VEE467 pKa = 3.62IVNGKK472 pKa = 7.28EE473 pKa = 3.76TLTATIHH480 pKa = 6.46KK481 pKa = 8.97SWNYY485 pKa = 8.89VANADD490 pKa = 3.99PVAMATQVKK499 pKa = 8.98FMCNLSKK506 pKa = 10.77FKK508 pKa = 10.66NHH510 pKa = 6.53PLINFILKK518 pKa = 9.24TDD520 pKa = 3.99DD521 pKa = 3.5VVKK524 pKa = 10.03TGVITPIPFGDD535 pKa = 4.25LYY537 pKa = 10.71RR538 pKa = 11.84YY539 pKa = 8.62TIMSGSALQGIHH551 pKa = 5.21EE552 pKa = 4.61TALQSIYY559 pKa = 10.81TLVPSLDD566 pKa = 3.37IVGEE570 pKa = 4.17KK571 pKa = 10.38

Molecular weight: 63.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291S0E7|A0A291S0E7_9VIRU Uncharacterized protein OS=Picobirnavirus sp. OX=1907787 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.11GGHH5 pKa = 5.91SFMTTQAIEE14 pKa = 3.96YY15 pKa = 9.04LKK17 pKa = 10.81LRR19 pKa = 11.84EE20 pKa = 4.15TEE22 pKa = 3.93RR23 pKa = 11.84SNKK26 pKa = 9.29ARR28 pKa = 11.84EE29 pKa = 4.28KK30 pKa = 8.47EE31 pKa = 4.11THH33 pKa = 6.3RR34 pKa = 11.84ANLAQEE40 pKa = 4.77NISTANLAEE49 pKa = 4.28TTRR52 pKa = 11.84SNKK55 pKa = 9.96AKK57 pKa = 10.45EE58 pKa = 4.35SISLDD63 pKa = 3.19TLAEE67 pKa = 4.31TKK69 pKa = 10.34RR70 pKa = 11.84SNQAKK75 pKa = 9.63EE76 pKa = 4.1AIEE79 pKa = 3.91TSKK82 pKa = 10.53FAEE85 pKa = 4.64TIRR88 pKa = 11.84SNLAKK93 pKa = 10.27EE94 pKa = 4.54FEE96 pKa = 4.52TQRR99 pKa = 11.84SNLAKK104 pKa = 9.72EE105 pKa = 4.26QEE107 pKa = 4.49TKK109 pKa = 10.47RR110 pKa = 11.84SNIAKK115 pKa = 9.18EE116 pKa = 3.83QEE118 pKa = 4.35TYY120 pKa = 10.34RR121 pKa = 11.84SNVARR126 pKa = 11.84EE127 pKa = 3.8RR128 pKa = 11.84EE129 pKa = 4.35TYY131 pKa = 9.88RR132 pKa = 11.84SNVAQEE138 pKa = 3.85SNTRR142 pKa = 11.84YY143 pKa = 8.15KK144 pKa = 10.89TNLDD148 pKa = 3.59NIGYY152 pKa = 9.48ISQAAALDD160 pKa = 3.79DD161 pKa = 3.8MGYY164 pKa = 10.4NPAKK168 pKa = 10.51SSLLMLYY175 pKa = 10.17DD176 pKa = 3.94AARR179 pKa = 11.84GLVPKK184 pKa = 10.6LSVSFKK190 pKa = 10.97

Molecular weight: 21.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1366

63

571

341.5

38.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.053 ± 1.422

1.098 ± 0.237

6.369 ± 0.969

5.124 ± 1.182

3.221 ± 0.424

5.198 ± 0.688

2.123 ± 0.561

6.735 ± 1.199

5.637 ± 0.922

7.613 ± 0.541

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.562 ± 0.298

6.369 ± 0.853

4.026 ± 0.574

3.807 ± 0.42

5.71 ± 0.832

7.394 ± 0.536

6.369 ± 0.714

6.003 ± 0.733

1.537 ± 0.531

5.051 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski