Puteibacter caeruleilacunae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Prolixibacteraceae; Puteibacter

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5722 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U2KS02|A0A4U2KS02_9BACT Uncharacterized protein OS=Puteibacter caeruleilacunae OX=2528631 GN=EYV94_28145 PE=4 SV=1
VV1 pKa = 6.56NADD4 pKa = 3.08AEE6 pKa = 4.55KK7 pKa = 10.28PVFSGCPNDD16 pKa = 3.7QLNVVMDD23 pKa = 4.55AGKK26 pKa = 10.58CGATITWTDD35 pKa = 3.18PTANDD40 pKa = 3.63NCDD43 pKa = 3.24GGITPVRR50 pKa = 11.84SDD52 pKa = 3.18ATGLNSGDD60 pKa = 4.34LFPEE64 pKa = 4.62GTTVISWTATDD75 pKa = 3.32AAGNVQTCSFTVTVNADD92 pKa = 2.98AEE94 pKa = 4.48KK95 pKa = 10.19PVFSGCPSDD104 pKa = 3.64QLNVVMDD111 pKa = 4.55AGKK114 pKa = 10.58CGATITWTDD123 pKa = 3.18PTANDD128 pKa = 3.64NCDD131 pKa = 3.29GAITPVRR138 pKa = 11.84SDD140 pKa = 3.29VTGLNSGDD148 pKa = 4.25LFPEE152 pKa = 4.62GTTVISWTATDD163 pKa = 3.32AAGNVQTCSFTVTVNADD180 pKa = 2.98AEE182 pKa = 4.48KK183 pKa = 10.19PVFSGCPNDD192 pKa = 3.7QLNVVMDD199 pKa = 4.47AGKK202 pKa = 10.55CC203 pKa = 3.48

Molecular weight: 20.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U2KJD5|A0A4U2KJD5_9BACT Enediyne biosynthesis protein (Fragment) OS=Puteibacter caeruleilacunae OX=2528631 GN=EYV94_28835 PE=4 SV=1
QQ1 pKa = 7.79FPAQRR6 pKa = 11.84NNXQQQQYY14 pKa = 9.57RR15 pKa = 11.84QANDD19 pKa = 3.23NRR21 pKa = 11.84CFTCGNTGHH30 pKa = 6.33YY31 pKa = 10.5AKK33 pKa = 10.45NCPRR37 pKa = 11.84NQQRR41 pKa = 11.84QGQNXNQNQGKK52 pKa = 7.76RR53 pKa = 11.84QKK55 pKa = 9.73VQVRR59 pKa = 11.84QGRR62 pKa = 11.84LNFTTMADD70 pKa = 3.44IPEE73 pKa = 4.55GAPVXTGIFTVLNYY87 pKa = 9.56PAIXLFDD94 pKa = 4.19SGASHH99 pKa = 6.86SFISAKK105 pKa = 10.31FSAKK109 pKa = 9.72CQLPFHH115 pKa = 6.52HH116 pKa = 7.43TDD118 pKa = 2.9GGITISTPGGRR129 pKa = 11.84VATYY133 pKa = 9.37QINRR137 pKa = 11.84HH138 pKa = 4.69VPIKK142 pKa = 10.39FGSLIIKK149 pKa = 7.49TT150 pKa = 3.87

Molecular weight: 16.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5722

0

5722

2334750

19

3404

408.0

46.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.119 ± 0.028

0.962 ± 0.012

5.91 ± 0.026

6.64 ± 0.025

4.798 ± 0.02

6.776 ± 0.032

1.939 ± 0.014

7.279 ± 0.03

7.497 ± 0.029

8.856 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.428 ± 0.015

5.829 ± 0.027

3.525 ± 0.016

3.445 ± 0.017

4.035 ± 0.019

6.432 ± 0.024

5.289 ± 0.026

6.441 ± 0.024

1.381 ± 0.012

4.391 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski