Bacillus phage PBC1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gutmannvirinae; Pebcunavirus; Bacillus virus PBC1

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1TLF8|I1TLF8_9CAUD Uncharacterized protein OS=Bacillus phage PBC1 OX=1161901 GN=PBC1_024 PE=4 SV=1
MM1 pKa = 7.51AKK3 pKa = 10.38YY4 pKa = 10.4YY5 pKa = 10.31GYY7 pKa = 10.87CYY9 pKa = 10.37DD10 pKa = 3.75EE11 pKa = 4.41NGKK14 pKa = 7.91FTEE17 pKa = 4.97MIPIDD22 pKa = 4.08EE23 pKa = 4.26KK24 pKa = 11.0PIYY27 pKa = 9.68EE28 pKa = 4.09KK29 pKa = 9.94QTFEE33 pKa = 4.72RR34 pKa = 11.84EE35 pKa = 3.95EE36 pKa = 3.88QKK38 pKa = 11.05EE39 pKa = 4.3VVTEE43 pKa = 4.09EE44 pKa = 4.41KK45 pKa = 10.8LCALHH50 pKa = 6.98QSIEE54 pKa = 4.18DD55 pKa = 3.54GTYY58 pKa = 10.24KK59 pKa = 10.76PEE61 pKa = 3.98IDD63 pKa = 3.69EE64 pKa = 4.46DD65 pKa = 3.88TGEE68 pKa = 4.16EE69 pKa = 4.14LPVISKK75 pKa = 8.25YY76 pKa = 10.15EE77 pKa = 4.09CPDD80 pKa = 3.5CVVAQVNYY88 pKa = 8.02EE89 pKa = 4.3TIKK92 pKa = 10.66VPYY95 pKa = 9.21EE96 pKa = 3.51EE97 pKa = 4.87DD98 pKa = 3.55VIVGYY103 pKa = 10.68EE104 pKa = 3.73PDD106 pKa = 3.7IPPNCTLEE114 pKa = 4.05VCPWLAYY121 pKa = 10.14EE122 pKa = 4.5SVFNGKK128 pKa = 8.1VWLKK132 pKa = 9.73TKK134 pKa = 9.6EE135 pKa = 4.15PKK137 pKa = 8.42PTPEE141 pKa = 5.45PIPEE145 pKa = 4.45PDD147 pKa = 3.3EE148 pKa = 4.77VEE150 pKa = 4.18LLRR153 pKa = 11.84DD154 pKa = 3.51EE155 pKa = 5.52LKK157 pKa = 9.39VTKK160 pKa = 10.32AALADD165 pKa = 4.96FIMQQALRR173 pKa = 11.84EE174 pKa = 4.24GLL176 pKa = 3.59

Molecular weight: 20.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1TLI2|I1TLI2_9CAUD p-loop containing nucleoside triphosphate hydrolase OS=Bacillus phage PBC1 OX=1161901 GN=PBC1_048 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 9.76IYY4 pKa = 10.73KK5 pKa = 9.77LAEE8 pKa = 3.84FALMLNVSSEE18 pKa = 4.07TLRR21 pKa = 11.84RR22 pKa = 11.84WDD24 pKa = 3.4RR25 pKa = 11.84SGKK28 pKa = 9.97LKK30 pKa = 10.9AFRR33 pKa = 11.84TPSNHH38 pKa = 6.32RR39 pKa = 11.84FYY41 pKa = 10.62THH43 pKa = 5.91EE44 pKa = 3.92QYY46 pKa = 10.2EE47 pKa = 4.18QYY49 pKa = 10.25MRR51 pKa = 11.84GNKK54 pKa = 9.09

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12575

49

1142

251.5

28.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.992 ± 0.632

0.692 ± 0.139

6.394 ± 0.414

7.046 ± 0.528

3.658 ± 0.191

6.457 ± 0.232

1.877 ± 0.163

6.362 ± 0.25

7.833 ± 0.374

7.682 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.268 ± 0.233

5.074 ± 0.359

3.753 ± 0.248

3.642 ± 0.239

4.27 ± 0.346

5.877 ± 0.317

5.996 ± 0.42

6.68 ± 0.262

1.479 ± 0.197

3.968 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski