Indian encephalitis associated cyclovirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; Human associated cyclovirus 12

Average proteome isoelectric point is 8.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7TEH5|A0A1N7TEH5_9CIRC Replication-associated protein OS=Indian encephalitis associated cyclovirus OX=1755290 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 5.14RR3 pKa = 11.84VTLKK7 pKa = 10.63RR8 pKa = 11.84FCFTLNNYY16 pKa = 10.45DD17 pKa = 3.62EE18 pKa = 5.32DD19 pKa = 3.74RR20 pKa = 11.84VKK22 pKa = 10.42ILKK25 pKa = 10.11EE26 pKa = 3.99FMSTKK31 pKa = 9.84CKK33 pKa = 8.81YY34 pKa = 10.62AIMGFEE40 pKa = 4.21EE41 pKa = 5.12APTTGTKK48 pKa = 10.1HH49 pKa = 6.03IQGSWLKK56 pKa = 10.18AACQLIIEE64 pKa = 4.84GKK66 pKa = 7.86SEE68 pKa = 3.9RR69 pKa = 11.84SIAEE73 pKa = 3.92QFPSQYY79 pKa = 11.1VMYY82 pKa = 10.48HH83 pKa = 6.55RR84 pKa = 11.84GFQALRR90 pKa = 11.84NAIAPPKK97 pKa = 9.95PRR99 pKa = 11.84DD100 pKa = 3.58FKK102 pKa = 10.79TEE104 pKa = 3.78VYY106 pKa = 10.63VYY108 pKa = 10.17IGPTGTGKK116 pKa = 10.0SRR118 pKa = 11.84KK119 pKa = 7.78AAEE122 pKa = 4.17MATQTNEE129 pKa = 4.04PIFYY133 pKa = 10.08KK134 pKa = 10.8ARR136 pKa = 11.84GNWWDD141 pKa = 3.96GYY143 pKa = 9.77KK144 pKa = 9.85QQEE147 pKa = 4.13NVIIDD152 pKa = 4.13DD153 pKa = 4.46FYY155 pKa = 11.76GWLEE159 pKa = 3.89LDD161 pKa = 3.98EE162 pKa = 4.72LLKK165 pKa = 10.06ITDD168 pKa = 4.14RR169 pKa = 11.84YY170 pKa = 9.19PYY172 pKa = 9.57RR173 pKa = 11.84VPVKK177 pKa = 10.41GSYY180 pKa = 9.35EE181 pKa = 3.99VFNSKK186 pKa = 10.53RR187 pKa = 11.84IWITSNKK194 pKa = 8.46TVTTWYY200 pKa = 7.47PTEE203 pKa = 4.29RR204 pKa = 11.84DD205 pKa = 3.71EE206 pKa = 6.41LLLALTRR213 pKa = 11.84RR214 pKa = 11.84FTQIEE219 pKa = 4.56YY220 pKa = 10.58FF221 pKa = 3.66

Molecular weight: 26.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7TEH5|A0A1N7TEH5_9CIRC Replication-associated protein OS=Indian encephalitis associated cyclovirus OX=1755290 PE=4 SV=1
MM1 pKa = 6.49WRR3 pKa = 11.84ARR5 pKa = 11.84RR6 pKa = 11.84GRR8 pKa = 11.84YY9 pKa = 9.11RR10 pKa = 11.84YY11 pKa = 9.17VGRR14 pKa = 11.84AGVYY18 pKa = 8.87KK19 pKa = 10.3NRR21 pKa = 11.84RR22 pKa = 11.84IYY24 pKa = 10.07RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84YY29 pKa = 9.52RR30 pKa = 11.84LPLYY34 pKa = 10.07RR35 pKa = 11.84PQRR38 pKa = 11.84LRR40 pKa = 11.84RR41 pKa = 11.84STGLDD46 pKa = 3.03VYY48 pKa = 10.88AVKK51 pKa = 10.24TEE53 pKa = 4.31VIVPTLDD60 pKa = 3.65DD61 pKa = 3.68NVIVLEE67 pKa = 4.55PYY69 pKa = 10.01ISDD72 pKa = 3.85FRR74 pKa = 11.84RR75 pKa = 11.84LADD78 pKa = 3.48TYY80 pKa = 11.68NLYY83 pKa = 10.7NPYY86 pKa = 10.48RR87 pKa = 11.84FMSLSVRR94 pKa = 11.84VVPWCNTTSSTVPNNLYY111 pKa = 10.41CCAPFHH117 pKa = 7.09RR118 pKa = 11.84PLTMKK123 pKa = 10.35VNEE126 pKa = 4.02ATYY129 pKa = 10.69VQILSLPRR137 pKa = 11.84SKK139 pKa = 10.52SYY141 pKa = 9.97PATRR145 pKa = 11.84SSYY148 pKa = 10.27RR149 pKa = 11.84RR150 pKa = 11.84FVPTVASVLSEE161 pKa = 4.26TGQSTVLTGSGLTRR175 pKa = 11.84YY176 pKa = 9.63RR177 pKa = 11.84PLISQSPGYY186 pKa = 9.9DD187 pKa = 2.96AAAVRR192 pKa = 11.84HH193 pKa = 5.19YY194 pKa = 10.61CAIYY198 pKa = 10.22KK199 pKa = 8.66FSPPASGQPPVEE211 pKa = 4.03YY212 pKa = 10.01PLILRR217 pKa = 11.84AHH219 pKa = 5.81VRR221 pKa = 11.84FFSPKK226 pKa = 9.62DD227 pKa = 3.34TDD229 pKa = 3.96FSS231 pKa = 4.0

Molecular weight: 26.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

452

221

231

226.0

26.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.973 ± 0.058

1.549 ± 0.121

3.761 ± 0.197

5.088 ± 1.932

4.425 ± 0.635

4.646 ± 0.496

1.106 ± 0.127

5.088 ± 1.074

5.31 ± 1.792

7.301 ± 0.611

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.77 ± 0.311

3.54 ± 0.051

6.637 ± 1.335

3.097 ± 0.616

10.177 ± 2.143

6.637 ± 1.621

7.743 ± 0.54

6.858 ± 1.475

1.77 ± 0.597

7.522 ± 0.751
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski