Microbacterium phage Arete

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Burrovirus; Microbacterium virus Arete

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R148|A0A6G8R148_9CAUD Uncharacterized protein OS=Microbacterium phage Arete OX=2713257 GN=39 PE=4 SV=1
MM1 pKa = 7.86SDD3 pKa = 4.0QEE5 pKa = 4.05WSDD8 pKa = 3.24AMTAIHH14 pKa = 7.04EE15 pKa = 4.39EE16 pKa = 4.12LDD18 pKa = 3.8MPIPAYY24 pKa = 10.95LNN26 pKa = 3.49

Molecular weight: 3.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R158|A0A6G8R158_9CAUD Uncharacterized protein OS=Microbacterium phage Arete OX=2713257 GN=46 PE=4 SV=1
MM1 pKa = 7.64SSEE4 pKa = 3.64IVQVKK9 pKa = 9.4RR10 pKa = 11.84QHH12 pKa = 5.59FRR14 pKa = 11.84QEE16 pKa = 3.42IPEE19 pKa = 4.12THH21 pKa = 6.48RR22 pKa = 11.84VSLDD26 pKa = 3.16TRR28 pKa = 11.84IRR30 pKa = 11.84WLWNQRR36 pKa = 11.84FGTVQSVYY44 pKa = 10.94KK45 pKa = 10.33NSKK48 pKa = 10.73DD49 pKa = 3.47MLDD52 pKa = 3.27RR53 pKa = 11.84TAATMFIQAIWARR66 pKa = 11.84DD67 pKa = 3.65MNSVQLILNRR77 pKa = 11.84LEE79 pKa = 4.62GGPITDD85 pKa = 3.63EE86 pKa = 4.25EE87 pKa = 4.51LVQRR91 pKa = 11.84QALRR95 pKa = 11.84VV96 pKa = 3.53

Molecular weight: 11.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

16868

26

4494

344.2

37.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.185 ± 0.578

0.427 ± 0.15

6.616 ± 0.217

6.035 ± 0.304

3.314 ± 0.14

7.885 ± 0.383

1.524 ± 0.176

4.677 ± 0.194

4.245 ± 0.333

8.057 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.555 ± 0.185

4.594 ± 0.239

4.986 ± 0.22

4.535 ± 0.168

5.922 ± 0.272

6.385 ± 0.289

6.486 ± 0.36

6.646 ± 0.227

1.873 ± 0.118

3.053 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski