Bacillus sp. YR335

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327RLA1|A0A327RLA1_9BACI Uncharacterized protein DUF4085 OS=Bacillus sp. YR335 OX=2135743 GN=C8K50_107112 PE=4 SV=1
MM1 pKa = 7.89DD2 pKa = 4.77KK3 pKa = 10.87EE4 pKa = 4.0ADD6 pKa = 3.54EE7 pKa = 4.41VFYY10 pKa = 10.81EE11 pKa = 4.64GNDD14 pKa = 3.38TTVFAGSVEE23 pKa = 4.13VLSEE27 pKa = 4.27VEE29 pKa = 3.81YY30 pKa = 10.81HH31 pKa = 6.14QLNDD35 pKa = 3.44SQLDD39 pKa = 3.82DD40 pKa = 4.63FFEE43 pKa = 5.29CYY45 pKa = 8.97EE46 pKa = 4.14QNEE49 pKa = 4.44EE50 pKa = 4.04TLIPQEE56 pKa = 4.06QKK58 pKa = 11.02VFTDD62 pKa = 3.72WFSQCWKK69 pKa = 10.19KK70 pKa = 11.04AGGDD74 pKa = 3.67ALDD77 pKa = 4.03LPSYY81 pKa = 10.89YY82 pKa = 10.55VFHH85 pKa = 7.95DD86 pKa = 4.25DD87 pKa = 4.73YY88 pKa = 11.91KK89 pKa = 11.67SFDD92 pKa = 3.78LKK94 pKa = 10.86NNQWIDD100 pKa = 4.43DD101 pKa = 3.99EE102 pKa = 4.99EE103 pKa = 4.35KK104 pKa = 10.18WSS106 pKa = 3.53

Molecular weight: 12.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327RMV8|A0A327RMV8_9BACI Phosphocarrier protein HPr OS=Bacillus sp. YR335 OX=2135743 GN=C8K50_10683 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.47VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTASGRR28 pKa = 11.84KK29 pKa = 7.99VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.94VLSAA44 pKa = 4.11

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5212

0

5212

1454700

29

3404

279.1

31.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.561 ± 0.04

0.751 ± 0.011

5.022 ± 0.03

7.422 ± 0.04

4.616 ± 0.028

6.784 ± 0.041

2.078 ± 0.018

8.274 ± 0.037

7.069 ± 0.037

9.811 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.703 ± 0.018

4.71 ± 0.029

3.489 ± 0.02

3.765 ± 0.027

3.804 ± 0.023

6.173 ± 0.025

5.484 ± 0.029

6.992 ± 0.029

0.988 ± 0.013

3.504 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski