Alloiococcus otitis ATCC 51267

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Alloiococcus; Alloiococcus otitis

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1627 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9EUT4|K9EUT4_9LACT Uncharacterized protein OS=Alloiococcus otitis ATCC 51267 OX=883081 GN=HMPREF9698_01533 PE=3 SV=1
MM1 pKa = 7.34SLKK4 pKa = 10.27KK5 pKa = 10.25KK6 pKa = 10.21YY7 pKa = 10.59AILASSAALSLTLAACGGEE26 pKa = 4.39GEE28 pKa = 4.66PVDD31 pKa = 4.31PGQEE35 pKa = 4.21GEE37 pKa = 4.39SSQEE41 pKa = 3.93TTEE44 pKa = 4.24DD45 pKa = 2.99QATQEE50 pKa = 4.2RR51 pKa = 11.84SPEE54 pKa = 4.11DD55 pKa = 3.26FDD57 pKa = 3.91EE58 pKa = 4.65TVQNEE63 pKa = 4.22GEE65 pKa = 4.56AIDD68 pKa = 4.76GGIAQVGVLSDD79 pKa = 3.79TAWTGIFDD87 pKa = 3.64WQHH90 pKa = 5.73YY91 pKa = 8.15EE92 pKa = 4.0NEE94 pKa = 4.13ADD96 pKa = 3.58ASLMNYY102 pKa = 8.38TLGDD106 pKa = 4.06LLAMNEE112 pKa = 3.95NYY114 pKa = 10.37EE115 pKa = 4.18LVGGKK120 pKa = 9.85EE121 pKa = 3.71NGAAADD127 pKa = 3.67IEE129 pKa = 4.22FDD131 pKa = 3.77EE132 pKa = 6.23DD133 pKa = 3.79NDD135 pKa = 4.26TVTITVHH142 pKa = 7.51DD143 pKa = 4.04DD144 pKa = 3.96VYY146 pKa = 10.79WHH148 pKa = 7.5DD149 pKa = 4.24GEE151 pKa = 5.39QVTADD156 pKa = 4.35DD157 pKa = 3.86IVFAHH162 pKa = 7.02EE163 pKa = 4.04IVGNPDD169 pKa = 3.1YY170 pKa = 11.26TGIRR174 pKa = 11.84YY175 pKa = 9.58GDD177 pKa = 3.67NFTNVQGMEE186 pKa = 4.07EE187 pKa = 4.41YY188 pKa = 10.52KK189 pKa = 10.84AGEE192 pKa = 4.13ADD194 pKa = 3.92SISGLEE200 pKa = 4.07LSDD203 pKa = 5.85DD204 pKa = 3.69EE205 pKa = 4.59MTVTIHH211 pKa = 5.42YY212 pKa = 8.21QQVVPSMKK220 pKa = 9.55QAGRR224 pKa = 11.84GVWAYY229 pKa = 9.69AAPRR233 pKa = 11.84HH234 pKa = 5.1YY235 pKa = 11.03LEE237 pKa = 6.27DD238 pKa = 3.56IPADD242 pKa = 3.58EE243 pKa = 5.05LVSHH247 pKa = 6.65PNVRR251 pKa = 11.84EE252 pKa = 3.82NPIGFGPFKK261 pKa = 10.59VDD263 pKa = 2.93NVVPGEE269 pKa = 4.17SVEE272 pKa = 3.93YY273 pKa = 10.1SAYY276 pKa = 10.05EE277 pKa = 4.28DD278 pKa = 3.69YY279 pKa = 11.23HH280 pKa = 8.16LGEE283 pKa = 5.1PKK285 pKa = 10.43LDD287 pKa = 4.61GITLEE292 pKa = 4.13RR293 pKa = 11.84LQPAGAIEE301 pKa = 4.03ALRR304 pKa = 11.84AGEE307 pKa = 4.19FDD309 pKa = 3.49WVDD312 pKa = 3.85SVPTDD317 pKa = 3.12QFDD320 pKa = 3.96IIEE323 pKa = 4.58EE324 pKa = 4.2GLPGYY329 pKa = 8.69TILGYY334 pKa = 9.23PDD336 pKa = 3.59RR337 pKa = 11.84GYY339 pKa = 11.38DD340 pKa = 3.51FLGFKK345 pKa = 10.36LGEE348 pKa = 3.81WDD350 pKa = 3.51ADD352 pKa = 3.66EE353 pKa = 6.04GKK355 pKa = 11.13VKK357 pKa = 10.33MNPDD361 pKa = 2.88AKK363 pKa = 10.43MADD366 pKa = 3.42KK367 pKa = 10.86NLRR370 pKa = 11.84QAMAYY375 pKa = 10.22ALDD378 pKa = 4.39LDD380 pKa = 4.02QVAITFSNGLSQRR393 pKa = 11.84ATSHH397 pKa = 5.98IPSNFASFYY406 pKa = 10.48RR407 pKa = 11.84DD408 pKa = 3.49DD409 pKa = 4.44LEE411 pKa = 4.8GYY413 pKa = 9.76PYY415 pKa = 11.09DD416 pKa = 4.35PDD418 pKa = 4.24KK419 pKa = 11.63ANQLLDD425 pKa = 3.38EE426 pKa = 5.28AGYY429 pKa = 11.18EE430 pKa = 4.15DD431 pKa = 4.23VDD433 pKa = 3.67GDD435 pKa = 4.62GFRR438 pKa = 11.84EE439 pKa = 4.48DD440 pKa = 4.64PDD442 pKa = 3.98GQPLEE447 pKa = 3.95ITYY450 pKa = 10.99AAMTGGDD457 pKa = 3.4TAEE460 pKa = 5.52PISQYY465 pKa = 11.15YY466 pKa = 8.2LQAWAEE472 pKa = 4.08VGLNVSLLEE481 pKa = 4.3GRR483 pKa = 11.84LHH485 pKa = 6.84EE486 pKa = 5.11SNSHH490 pKa = 5.34YY491 pKa = 11.12DD492 pKa = 3.91RR493 pKa = 11.84IQGDD497 pKa = 3.82DD498 pKa = 3.59PEE500 pKa = 5.95IDD502 pKa = 3.47VFEE505 pKa = 5.74AGFGALSDD513 pKa = 3.85PTPSVIYY520 pKa = 10.48GPNEE524 pKa = 4.3PINFTRR530 pKa = 11.84YY531 pKa = 7.69EE532 pKa = 3.83TDD534 pKa = 2.93EE535 pKa = 4.2NNEE538 pKa = 3.68YY539 pKa = 10.66LQAFEE544 pKa = 5.0ADD546 pKa = 3.31EE547 pKa = 5.52AFDD550 pKa = 4.72DD551 pKa = 5.64DD552 pKa = 4.3YY553 pKa = 11.5RR554 pKa = 11.84QQLFHH559 pKa = 7.37DD560 pKa = 3.72WQEE563 pKa = 4.04FFMDD567 pKa = 5.62EE568 pKa = 4.4IPTLPTFWFTQLEE581 pKa = 4.41IVNDD585 pKa = 3.82RR586 pKa = 11.84VSAYY590 pKa = 9.81AHH592 pKa = 6.11SSVPQVDD599 pKa = 3.07QDD601 pKa = 3.81TYY603 pKa = 10.76GLHH606 pKa = 6.62EE607 pKa = 4.82IEE609 pKa = 5.32LLAEE613 pKa = 4.39EE614 pKa = 5.22PLTEE618 pKa = 4.0

Molecular weight: 68.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9EAN6|K9EAN6_9LACT Uncharacterized protein OS=Alloiococcus otitis ATCC 51267 OX=883081 GN=HMPREF9698_00331 PE=4 SV=1
MM1 pKa = 7.42AQGLTFNPKK10 pKa = 9.07KK11 pKa = 10.58RR12 pKa = 11.84KK13 pKa = 8.92GKK15 pKa = 9.67KK16 pKa = 7.8NHH18 pKa = 5.53GFRR21 pKa = 11.84KK22 pKa = 9.98RR23 pKa = 11.84MSTKK27 pKa = 10.18NGRR30 pKa = 11.84KK31 pKa = 8.49ILKK34 pKa = 8.57NRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 8.72GRR41 pKa = 11.84KK42 pKa = 7.92RR43 pKa = 11.84LSYY46 pKa = 11.02

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1627

0

1627

512849

35

2489

315.2

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.365 ± 0.051

0.464 ± 0.014

7.267 ± 0.065

6.927 ± 0.083

4.186 ± 0.05

6.919 ± 0.054

1.852 ± 0.029

7.028 ± 0.068

5.935 ± 0.071

10.154 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.25 ± 0.029

4.236 ± 0.039

3.584 ± 0.041

5.304 ± 0.054

4.073 ± 0.051

6.227 ± 0.043

4.945 ± 0.036

6.865 ± 0.048

0.804 ± 0.02

3.616 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski