Ralstonia phage phiITL-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Serkorvirus; Ralstonia virus ITL1

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1ZDK1|A0A0U1ZDK1_9CAUD Uncharacterized protein OS=Ralstonia phage phiITL-1 OX=1597967 PE=4 SV=1
MM1 pKa = 7.7AIIVTPRR8 pKa = 11.84TEE10 pKa = 3.94LDD12 pKa = 3.21AVNAIIGAIGEE23 pKa = 4.55GVVNTLEE30 pKa = 4.12GDD32 pKa = 3.76ANVDD36 pKa = 3.3VLNARR41 pKa = 11.84RR42 pKa = 11.84LLAVVSTEE50 pKa = 4.07VQDD53 pKa = 4.22KK54 pKa = 10.51GWTFNVDD61 pKa = 2.87EE62 pKa = 5.07AFQLVPDD69 pKa = 4.54TFSSKK74 pKa = 10.34IVWLPTYY81 pKa = 10.4LRR83 pKa = 11.84VITAGGTPYY92 pKa = 10.59VNRR95 pKa = 11.84GGFVYY100 pKa = 10.54DD101 pKa = 3.38RR102 pKa = 11.84LNRR105 pKa = 11.84TDD107 pKa = 3.52QFPGRR112 pKa = 11.84ITVTMTEE119 pKa = 3.85QVPFEE124 pKa = 4.08EE125 pKa = 5.34LPLCFRR131 pKa = 11.84QYY133 pKa = 8.88ITYY136 pKa = 10.12IAADD140 pKa = 3.76RR141 pKa = 11.84FNSQFYY147 pKa = 10.13GDD149 pKa = 4.32PGVSEE154 pKa = 4.75ACAKK158 pKa = 10.62AIIEE162 pKa = 4.35ASNSVQEE169 pKa = 3.97YY170 pKa = 8.99EE171 pKa = 4.09IDD173 pKa = 3.46YY174 pKa = 11.22GGYY177 pKa = 10.4NLFNNDD183 pKa = 3.78PYY185 pKa = 11.37FLANSGRR192 pKa = 3.63

Molecular weight: 21.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1ZI53|A0A0U1ZI53_9CAUD DNA primase/helicase-like protein OS=Ralstonia phage phiITL-1 OX=1597967 PE=4 SV=1
MM1 pKa = 7.54NLRR4 pKa = 11.84NAPALGYY11 pKa = 8.99TYY13 pKa = 11.26AEE15 pKa = 3.74ARR17 pKa = 11.84AAGFLSATSPEE28 pKa = 3.79AWAATQQVNHH38 pKa = 5.93MVLTATDD45 pKa = 4.03PVLKK49 pKa = 10.59GQAEE53 pKa = 4.03EE54 pKa = 4.2RR55 pKa = 11.84ARR57 pKa = 11.84KK58 pKa = 8.89ALKK61 pKa = 10.41DD62 pKa = 3.43SLRR65 pKa = 11.84RR66 pKa = 11.84AMGMAATGHH75 pKa = 6.14GPVGVHH81 pKa = 6.18RR82 pKa = 11.84KK83 pKa = 8.09GTITVSRR90 pKa = 11.84EE91 pKa = 3.31EE92 pKa = 3.86ARR94 pKa = 11.84AA95 pKa = 3.33

Molecular weight: 10.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11670

52

1294

216.1

23.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.285 ± 0.528

1.011 ± 0.226

5.827 ± 0.261

6.401 ± 0.271

3.385 ± 0.163

8.423 ± 0.378

2.014 ± 0.288

4.516 ± 0.212

5.338 ± 0.319

7.669 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.128

3.599 ± 0.269

4.687 ± 0.263

4.045 ± 0.347

6.71 ± 0.281

5.553 ± 0.279

5.698 ± 0.293

7.232 ± 0.204

1.568 ± 0.156

2.768 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski