Paraburkholderia lacunae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7264 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370MZH9|A0A370MZH9_9BURK Ribonuclease Z OS=Paraburkholderia lacunae OX=2211104 GN=DLM46_32170 PE=4 SV=1
MM1 pKa = 7.96EE2 pKa = 4.4YY3 pKa = 10.45QSWMCLICGWIYY15 pKa = 11.01DD16 pKa = 4.28EE17 pKa = 4.8EE18 pKa = 6.26AGLPDD23 pKa = 3.89EE24 pKa = 5.81GIAPGTRR31 pKa = 11.84WEE33 pKa = 4.49DD34 pKa = 3.51VPINWTCPEE43 pKa = 3.97CGARR47 pKa = 11.84KK48 pKa = 9.54EE49 pKa = 4.07DD50 pKa = 3.87FEE52 pKa = 4.52MVQII56 pKa = 4.59

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370NEN5|A0A370NEN5_9BURK H-NS histone OS=Paraburkholderia lacunae OX=2211104 GN=DLM46_03560 PE=3 SV=1
MM1 pKa = 7.11HH2 pKa = 7.28TLLSTRR8 pKa = 11.84LTRR11 pKa = 11.84AALKK15 pKa = 10.04AARR18 pKa = 11.84PARR21 pKa = 11.84RR22 pKa = 11.84HH23 pKa = 3.86VVRR26 pKa = 11.84ALRR29 pKa = 11.84HH30 pKa = 4.85HH31 pKa = 5.94SARR34 pKa = 11.84TRR36 pKa = 11.84ALGEE40 pKa = 3.58QWRR43 pKa = 11.84DD44 pKa = 3.02AKK46 pKa = 11.03AVDD49 pKa = 5.51GIRR52 pKa = 11.84TWFHH56 pKa = 5.73TFFAALRR63 pKa = 11.84VQGAARR69 pKa = 11.84RR70 pKa = 11.84FSLRR74 pKa = 11.84TLLRR78 pKa = 11.84KK79 pKa = 7.49PTPTRR84 pKa = 11.84ALTATRR90 pKa = 11.84RR91 pKa = 11.84APVAALQSTSRR102 pKa = 11.84RR103 pKa = 11.84PRR105 pKa = 11.84RR106 pKa = 11.84MSTSGSTGWFAFAFASRR123 pKa = 3.71

Molecular weight: 13.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7264

0

7264

2306885

24

4578

317.6

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.618 ± 0.039

0.959 ± 0.01

5.351 ± 0.022

5.142 ± 0.029

3.721 ± 0.02

8.16 ± 0.027

2.325 ± 0.013

4.748 ± 0.021

3.108 ± 0.022

10.233 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.348 ± 0.012

2.849 ± 0.022

5.066 ± 0.021

3.676 ± 0.019

6.891 ± 0.028

5.794 ± 0.022

5.454 ± 0.022

7.757 ± 0.024

1.364 ± 0.012

2.436 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski