Candidatus Fukatsuia symbiotica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Yersiniaceae; Candidatus Fukatsuia

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8I3S7|A0A2U8I3S7_9GAMM Arginine ABC transporter ATP-binding protein ArtP OS=Candidatus Fukatsuia symbiotica OX=1878942 GN=CCS41_03630 PE=3 SV=1
MM1 pKa = 7.58SSLNALDD8 pKa = 5.01FKK10 pKa = 11.69VPDD13 pKa = 4.07LALLRR18 pKa = 11.84CWLSQLGCPFFEE30 pKa = 5.67CDD32 pKa = 2.94SCQALHH38 pKa = 6.61LCHH41 pKa = 5.94MQKK44 pKa = 10.55CDD46 pKa = 3.35GVSDD50 pKa = 4.06ARR52 pKa = 11.84IDD54 pKa = 4.03LKK56 pKa = 11.34DD57 pKa = 3.71DD58 pKa = 4.2VILFSVLAEE67 pKa = 3.97IRR69 pKa = 11.84PTALIPLSSNLSQINTLSLTAKK91 pKa = 10.26AFINIEE97 pKa = 4.14EE98 pKa = 4.37NDD100 pKa = 3.63NLAQLIVCQSISIATGITEE119 pKa = 3.84QQFDD123 pKa = 3.7HH124 pKa = 7.03FMQQSTEE131 pKa = 4.11QISMIISTVAEE142 pKa = 3.85NDD144 pKa = 3.48MLFLSDD150 pKa = 4.03DD151 pKa = 3.79YY152 pKa = 11.4EE153 pKa = 5.04YY154 pKa = 11.19EE155 pKa = 4.2DD156 pKa = 5.22DD157 pKa = 4.06EE158 pKa = 4.72EE159 pKa = 4.61EE160 pKa = 4.31EE161 pKa = 4.26EE162 pKa = 4.56EE163 pKa = 4.32EE164 pKa = 4.31EE165 pKa = 4.48EE166 pKa = 4.47KK167 pKa = 11.09NSPPVSRR174 pKa = 11.84FRR176 pKa = 11.84PIIHH180 pKa = 6.94

Molecular weight: 20.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8I7F4|A0A2U8I7F4_9GAMM Uncharacterized protein OS=Candidatus Fukatsuia symbiotica OX=1878942 GN=CCS41_11980 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22SRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2350

0

2350

694485

16

3391

295.5

33.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.193 ± 0.054

1.128 ± 0.024

5.19 ± 0.039

5.677 ± 0.059

3.768 ± 0.034

6.263 ± 0.051

2.492 ± 0.029

6.772 ± 0.048

5.713 ± 0.061

10.783 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.022

4.467 ± 0.047

4.037 ± 0.041

4.994 ± 0.047

5.539 ± 0.043

6.32 ± 0.048

5.682 ± 0.042

6.242 ± 0.043

1.24 ± 0.022

3.088 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski