Micromonas pusilla (strain CCMP1545) (Picoplanktonic green alga)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales; Mamiellaceae; Micromonas; Micromonas pusilla

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C1MWV0|C1MWV0_MICPC Predicted protein OS=Micromonas pusilla (strain CCMP1545) OX=564608 GN=MICPUCDRAFT_59262 PE=4 SV=1
MM1 pKa = 6.75TRR3 pKa = 11.84GRR5 pKa = 11.84ARR7 pKa = 11.84DD8 pKa = 3.38AAAAAAAAARR18 pKa = 11.84VAVLLLLLLLARR30 pKa = 11.84AASAQSPPPRR40 pKa = 11.84SRR42 pKa = 11.84SPAFGSCVDD51 pKa = 3.43ACGSCWYY58 pKa = 10.16EE59 pKa = 3.62RR60 pKa = 11.84DD61 pKa = 3.5DD62 pKa = 3.94PSRR65 pKa = 11.84VSCCCEE71 pKa = 3.87PTCADD76 pKa = 5.5AGDD79 pKa = 4.37CCDD82 pKa = 5.68DD83 pKa = 3.63YY84 pKa = 12.28AEE86 pKa = 4.38ACEE89 pKa = 4.84ANGDD93 pKa = 3.99DD94 pKa = 4.12EE95 pKa = 6.11RR96 pKa = 11.84GGDD99 pKa = 3.87GEE101 pKa = 4.43RR102 pKa = 11.84GVAGVVAEE110 pKa = 4.45ANGDD114 pKa = 3.56AALFFAPAPGPGASALFAAPIAVDD138 pKa = 3.78VEE140 pKa = 4.43DD141 pKa = 5.71DD142 pKa = 4.22ADD144 pKa = 4.18DD145 pKa = 4.63DD146 pKa = 4.66APVRR150 pKa = 11.84APDD153 pKa = 4.21PPTAAPAPSPSSVAYY168 pKa = 8.8FVAPAEE174 pKa = 4.16EE175 pKa = 4.33EE176 pKa = 4.21EE177 pKa = 4.43EE178 pKa = 4.27EE179 pKa = 4.68EE180 pKa = 4.21EE181 pKa = 4.56TEE183 pKa = 4.11TDD185 pKa = 3.07ATAAPPPTPRR195 pKa = 11.84VVPTIPEE202 pKa = 4.2EE203 pKa = 4.22PSPSPPSPSPPSPSPPSPSPPPPSPSPPPIAPPPPVVEE241 pKa = 4.4EE242 pKa = 4.35GVVADD247 pKa = 4.78VVEE250 pKa = 4.53EE251 pKa = 4.1DD252 pKa = 5.71DD253 pKa = 5.82GYY255 pKa = 11.81QNQNQNDD262 pKa = 3.96YY263 pKa = 11.68DD264 pKa = 5.58DD265 pKa = 6.41DD266 pKa = 7.22DD267 pKa = 7.47DD268 pKa = 7.66DD269 pKa = 7.62DD270 pKa = 7.01DD271 pKa = 7.22DD272 pKa = 5.72EE273 pKa = 7.46DD274 pKa = 4.65DD275 pKa = 4.3AVLVDD280 pKa = 3.64VSGGAGGVLPTAKK293 pKa = 9.89FWEE296 pKa = 4.64AVADD300 pKa = 4.42RR301 pKa = 11.84LGLDD305 pKa = 4.2LRR307 pKa = 11.84GNPAKK312 pKa = 10.53EE313 pKa = 4.07EE314 pKa = 3.96EE315 pKa = 4.5SEE317 pKa = 4.28LEE319 pKa = 3.95QEE321 pKa = 4.21EE322 pKa = 4.72EE323 pKa = 3.84EE324 pKa = 4.99DD325 pKa = 4.01EE326 pKa = 4.27EE327 pKa = 5.24EE328 pKa = 4.2KK329 pKa = 10.63TKK331 pKa = 11.28DD332 pKa = 3.14DD333 pKa = 3.72APRR336 pKa = 11.84VGGGGPPKK344 pKa = 10.51ASTGRR349 pKa = 11.84RR350 pKa = 11.84DD351 pKa = 5.35DD352 pKa = 5.52DD353 pKa = 6.11DD354 pKa = 7.14DD355 pKa = 4.31DD356 pKa = 5.76ANPISTAGEE365 pKa = 3.8NAFCLDD371 pKa = 3.48KK372 pKa = 11.51SEE374 pKa = 3.98EE375 pKa = 4.04RR376 pKa = 11.84YY377 pKa = 10.11YY378 pKa = 11.55ADD380 pKa = 2.97TTRR383 pKa = 11.84ACDD386 pKa = 4.19CDD388 pKa = 4.9CAGCDD393 pKa = 3.29CRR395 pKa = 11.84RR396 pKa = 11.84YY397 pKa = 8.59YY398 pKa = 11.09ACWDD402 pKa = 3.72CDD404 pKa = 3.35GGACASQASYY414 pKa = 10.78EE415 pKa = 4.39CARR418 pKa = 11.84GDD420 pKa = 3.23YY421 pKa = 10.01STFNEE426 pKa = 4.38KK427 pKa = 10.55LQLCAWEE434 pKa = 4.38QPRR437 pKa = 11.84PLPFSCPPRR446 pKa = 11.84PPGPPNPPNPPRR458 pKa = 11.84PPPRR462 pKa = 11.84PAAPPPLPGFPAAPPNPPALAPLPTPLPQPTLQPGVVPGGGVGGFPGGGVAQPGLGGGVVGGVPGAGAGVGQPGAGVGQPSQFQPGFGAQPGFGAQPGFGAQPGGAVSPVQTQPGSCASIPQCNSCLQSSDD593 pKa = 3.03QSSFVCCCDD602 pKa = 3.95PDD604 pKa = 4.28CSTWNANAPGQPAGFLGCCSDD625 pKa = 3.8YY626 pKa = 11.48AATCGAGQAIPGRR639 pKa = 11.84QQRR642 pKa = 11.84GLLL645 pKa = 3.72

Molecular weight: 65.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C1N6Q0|C1N6Q0_MICPC Predicted protein OS=Micromonas pusilla (strain CCMP1545) OX=564608 GN=MICPUCDRAFT_53390 PE=4 SV=1
MM1 pKa = 6.94TTTTTTTTTRR11 pKa = 11.84RR12 pKa = 11.84TKK14 pKa = 8.58RR15 pKa = 11.84TKK17 pKa = 10.47RR18 pKa = 11.84RR19 pKa = 11.84TKK21 pKa = 9.07RR22 pKa = 11.84TKK24 pKa = 8.66TRR26 pKa = 11.84ARR28 pKa = 11.84RR29 pKa = 11.84FKK31 pKa = 10.23PRR33 pKa = 11.84TRR35 pKa = 11.84TRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84PRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84SRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84PALGLLKK59 pKa = 10.32RR60 pKa = 11.84RR61 pKa = 11.84SRR63 pKa = 11.84LAIAPRR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84TSTFRR77 pKa = 11.84GARR80 pKa = 11.84WRR82 pKa = 11.84RR83 pKa = 11.84CSPRR87 pKa = 11.84WATRR91 pKa = 11.84SRR93 pKa = 11.84SSRR96 pKa = 11.84PRR98 pKa = 11.84RR99 pKa = 11.84SRR101 pKa = 11.84ARR103 pKa = 11.84ARR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84RR121 pKa = 11.84PTLPCRR127 pKa = 11.84TRR129 pKa = 11.84CRR131 pKa = 11.84TRR133 pKa = 11.84PMTRR137 pKa = 11.84LATRR141 pKa = 11.84WRR143 pKa = 11.84PRR145 pKa = 11.84PPRR148 pKa = 11.84WTRR151 pKa = 11.84STPRR155 pKa = 11.84SPRR158 pKa = 11.84SS159 pKa = 3.19

Molecular weight: 20.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10246

4

10250

4600988

35

7926

448.9

48.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.193 ± 0.064

1.431 ± 0.012

6.678 ± 0.023

6.499 ± 0.029

3.094 ± 0.015

8.59 ± 0.036

1.951 ± 0.012

2.987 ± 0.017

4.354 ± 0.03

7.707 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.99 ± 0.012

2.494 ± 0.014

5.48 ± 0.027

2.069 ± 0.016

8.129 ± 0.033

6.74 ± 0.025

5.341 ± 0.019

7.12 ± 0.023

1.208 ± 0.01

1.942 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski