Bovine polyomavirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; unclassified Polyomaviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A089QEX6|A0A089QEX6_9POLY Small T antigen OS=Bovine polyomavirus 2 OX=1578134 PE=4 SV=1
MM1 pKa = 7.41GAFISLLLNVGALAAEE17 pKa = 4.99LSAEE21 pKa = 4.17TAISVEE27 pKa = 4.41SILTGEE33 pKa = 4.25ALAALEE39 pKa = 4.35AEE41 pKa = 4.71IASVMTIQGISGIEE55 pKa = 4.01ALTQLGFTAEE65 pKa = 4.07QFSNFSLVSSLVNQALQYY83 pKa = 11.25AFYY86 pKa = 9.41FQTVSGAAALVEE98 pKa = 5.16AGIKK102 pKa = 8.89WGLGEE107 pKa = 4.34RR108 pKa = 11.84SVVDD112 pKa = 3.86ASDD115 pKa = 3.58VGLSPNGRR123 pKa = 11.84LLRR126 pKa = 11.84EE127 pKa = 3.7FAKK130 pKa = 10.59GFKK133 pKa = 9.41IDD135 pKa = 3.85PFNWSQSLIHH145 pKa = 6.56NIGRR149 pKa = 11.84TIFEE153 pKa = 5.28KK154 pKa = 10.53ISAHH158 pKa = 5.83NKK160 pKa = 6.66ITQATAQNYY169 pKa = 9.05ARR171 pKa = 11.84LIEE174 pKa = 3.88MGRR177 pKa = 11.84WYY179 pKa = 10.87VEE181 pKa = 4.04STDD184 pKa = 4.33SVTAGNDD191 pKa = 3.06SGLSVAVYY199 pKa = 7.28PAPGGAEE206 pKa = 3.79QRR208 pKa = 11.84ATPDD212 pKa = 2.71WLLPLILGLSGDD224 pKa = 4.0TTPTWKK230 pKa = 10.5ADD232 pKa = 3.58LEE234 pKa = 4.77INGSS238 pKa = 3.49

Molecular weight: 25.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A089RS32|A0A089RS32_9POLY VP1 OS=Bovine polyomavirus 2 OX=1578134 PE=3 SV=1
MM1 pKa = 7.99DD2 pKa = 6.15SILSRR7 pKa = 11.84QEE9 pKa = 3.63KK10 pKa = 10.49RR11 pKa = 11.84EE12 pKa = 3.98LCDD15 pKa = 3.54LLEE18 pKa = 4.82IPHH21 pKa = 6.66HH22 pKa = 6.83CYY24 pKa = 11.18GNIPMMKK31 pKa = 9.43AQYY34 pKa = 11.28KK35 pKa = 9.96KK36 pKa = 9.57MCLLYY41 pKa = 10.77HH42 pKa = 6.92PDD44 pKa = 3.64KK45 pKa = 11.46GGDD48 pKa = 3.38GAKK51 pKa = 9.02MVKK54 pKa = 10.2LNSLWTSFQDD64 pKa = 3.28EE65 pKa = 4.63VTKK68 pKa = 10.92LRR70 pKa = 11.84AEE72 pKa = 4.3VNFFSYY78 pKa = 10.14QVSAKK83 pKa = 9.8FFWDD87 pKa = 3.45LDD89 pKa = 3.87FNTLKK94 pKa = 10.68EE95 pKa = 4.18YY96 pKa = 10.72LGKK99 pKa = 9.76NQCKK103 pKa = 9.56FLRR106 pKa = 11.84GPNCLNSKK114 pKa = 10.22YY115 pKa = 10.34SYY117 pKa = 10.1CRR119 pKa = 11.84CIVCRR124 pKa = 11.84LSQQHH129 pKa = 5.93QDD131 pKa = 3.07IKK133 pKa = 10.81DD134 pKa = 3.75TQEE137 pKa = 3.99KK138 pKa = 10.22KK139 pKa = 10.25CLIWGEE145 pKa = 4.45CYY147 pKa = 10.37CYY149 pKa = 10.05RR150 pKa = 11.84CYY152 pKa = 10.92LLWFGFPPTWEE163 pKa = 4.04TFDD166 pKa = 3.39WYY168 pKa = 11.13QEE170 pKa = 4.31IILNTDD176 pKa = 2.81MHH178 pKa = 7.17LLRR181 pKa = 11.84LFNYY185 pKa = 10.02CKK187 pKa = 9.85YY188 pKa = 10.57QPGGDD193 pKa = 3.21SHH195 pKa = 7.79QPGKK199 pKa = 10.83LCC201 pKa = 5.09

Molecular weight: 23.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2014

193

781

335.7

37.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.703 ± 1.144

2.334 ± 0.577

4.667 ± 0.397

6.504 ± 0.46

4.171 ± 0.61

6.703 ± 0.919

2.135 ± 0.519

5.065 ± 0.451

6.455 ± 1.082

9.732 ± 0.51

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.936 ± 0.406

4.27 ± 0.242

6.604 ± 1.54

4.568 ± 0.648

4.518 ± 0.601

8.193 ± 1.173

5.76 ± 0.342

4.965 ± 0.675

1.44 ± 0.318

3.277 ± 0.662
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski