Pectobacterium phage DU_PP_II

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Unyawovirus; Pectobacterium virus DUPPII

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D2W5W2|A0A2D2W5W2_9CAUD Inhibitor of host bacterial RNA polymerase OS=Pectobacterium phage DU_PP_II OX=2041489 GN=P2B40kb_p010 PE=4 SV=1
MM1 pKa = 7.7AEE3 pKa = 4.14SNADD7 pKa = 3.26VYY9 pKa = 11.72ASFGVNSAVVTGGSQEE25 pKa = 3.99EE26 pKa = 4.58HH27 pKa = 5.48TQNMLALDD35 pKa = 3.7VATRR39 pKa = 11.84DD40 pKa = 3.47GDD42 pKa = 3.96DD43 pKa = 4.91LISITEE49 pKa = 4.11DD50 pKa = 3.54APVDD54 pKa = 4.27LYY56 pKa = 11.82ANSDD60 pKa = 3.3KK61 pKa = 10.73FANPEE66 pKa = 4.1DD67 pKa = 4.34DD68 pKa = 3.85NGFIQVRR75 pKa = 11.84IGDD78 pKa = 3.81GSDD81 pKa = 3.59PEE83 pKa = 4.52GQTTGQPEE91 pKa = 4.46VTTTDD96 pKa = 3.22DD97 pKa = 4.97VEE99 pKa = 4.29FQQLGEE105 pKa = 4.25IPTNLTDD112 pKa = 3.89TSQKK116 pKa = 10.64LADD119 pKa = 4.32HH120 pKa = 6.52EE121 pKa = 5.59AGFQTIVAQAQEE133 pKa = 4.28KK134 pKa = 9.88GVPEE138 pKa = 4.21DD139 pKa = 3.64SITRR143 pKa = 11.84IQSEE147 pKa = 4.38YY148 pKa = 10.24QGDD151 pKa = 4.37GISEE155 pKa = 3.68QSYY158 pKa = 11.25AEE160 pKa = 4.34LEE162 pKa = 3.93AAGYY166 pKa = 9.82SRR168 pKa = 11.84QFVDD172 pKa = 4.72SYY174 pKa = 11.59ISGQEE179 pKa = 3.97ALVDD183 pKa = 3.99AYY185 pKa = 11.19VNQVMDD191 pKa = 3.8FAGGRR196 pKa = 11.84EE197 pKa = 4.04AFQAVHH203 pKa = 5.72AHH205 pKa = 5.38MVATNPEE212 pKa = 3.86AAQTFEE218 pKa = 4.14TALGNRR224 pKa = 11.84DD225 pKa = 3.92MVTMKK230 pKa = 11.01ALLNLAGQSRR240 pKa = 11.84TKK242 pKa = 11.07AFGKK246 pKa = 9.05PAEE249 pKa = 4.18RR250 pKa = 11.84SMISKK255 pKa = 9.29GAPASPAKK263 pKa = 10.33PEE265 pKa = 4.01GKK267 pKa = 10.35AKK269 pKa = 10.58VEE271 pKa = 4.1PFASQQEE278 pKa = 4.58LIKK281 pKa = 11.12AMSDD285 pKa = 2.75DD286 pKa = 4.39RR287 pKa = 11.84YY288 pKa = 10.82RR289 pKa = 11.84SDD291 pKa = 2.79AAYY294 pKa = 10.0RR295 pKa = 11.84RR296 pKa = 11.84SVEE299 pKa = 3.73LRR301 pKa = 11.84VINSAFF307 pKa = 3.16

Molecular weight: 33.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D2W5U2|A0A2D2W5U2_9CAUD Serine recombinase OS=Pectobacterium phage DU_PP_II OX=2041489 GN=P2B40kb_p001 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84LHH4 pKa = 6.56YY5 pKa = 10.32NSSNGIFSVRR15 pKa = 11.84RR16 pKa = 11.84PDD18 pKa = 4.19RR19 pKa = 11.84STQSASGKK27 pKa = 8.08HH28 pKa = 4.78QKK30 pKa = 10.6LPIIGDD36 pKa = 3.62IVEE39 pKa = 4.7LSPKK43 pKa = 10.02VFLLITRR50 pKa = 11.84GEE52 pKa = 4.09FVEE55 pKa = 4.38ATKK58 pKa = 9.45GTRR61 pKa = 11.84PFMQAVVTSWPKK73 pKa = 8.53TRR75 pKa = 11.84LVIKK79 pKa = 10.13RR80 pKa = 11.84IKK82 pKa = 10.22EE83 pKa = 3.78IIKK86 pKa = 10.44

Molecular weight: 9.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

11648

48

1328

277.3

30.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.989 ± 0.522

0.91 ± 0.172

6.053 ± 0.179

6.533 ± 0.318

3.735 ± 0.166

7.95 ± 0.287

1.743 ± 0.176

5.263 ± 0.142

6.156 ± 0.387

8.079 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.174

4.507 ± 0.295

3.872 ± 0.228

4.301 ± 0.319

5.237 ± 0.181

6.585 ± 0.256

5.958 ± 0.285

6.739 ± 0.311

1.52 ± 0.164

3.254 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski