Proteome-pI 2.0 - Proteome Isoelectric Point Database

Amycolatopsis cihanbeyliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 6558 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A542DI10|A0A542DI10_9PSEU Molybdenum cofactor synthesis domain-containing protein OS=Amycolatopsis cihanbeyliensis OX=1128664 GN=FB471_2473 PE=4 SV=1

MM1 pKa = 7.46PVVIALVVSTAWIVQFIGSPDD22 pKa = 3.19RR23 pKa = 11.84AEE25 pKa = 5.37AGDD28 pKa = 4.02CLAIAEE34 pKa = 4.49FTDD37 pKa = 3.99DD38 pKa = 4.64ADD40 pKa = 4.59VEE42 pKa = 4.78TVDD45 pKa = 5.36CADD48 pKa = 3.55PSANVKK54 pKa = 10.55VGVKK58 pKa = 9.94LDD60 pKa = 3.78SAADD64 pKa = 3.82RR65 pKa = 11.84CPGPNYY71 pKa = 10.41DD72 pKa = 3.88EE73 pKa = 5.12LVLEE77 pKa = 4.84GEE79 pKa = 4.51AGYY82 pKa = 10.45KK83 pKa = 9.53LCLMLHH89 pKa = 6.15VSAGDD94 pKa = 3.55CLANLDD100 pKa = 3.6QSTTKK105 pKa = 10.52GYY107 pKa = 7.89EE108 pKa = 4.0TVPCGDD114 pKa = 4.27PSAQAEE120 pKa = 4.16VLEE123 pKa = 4.44IVEE126 pKa = 4.8GDD128 pKa = 3.53PDD130 pKa = 3.71AQEE133 pKa = 4.48ACAQAGGDD141 pKa = 3.44VMGYY145 pKa = 9.17PEE147 pKa = 5.12PPRR150 pKa = 11.84SLCMRR155 pKa = 11.84APRR158 pKa = 11.84TAA160 pKa = 3.18

Molecular weight: 16.69 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A542DLR5|A0A542DLR5_9PSEU Uncharacterized protein OS=Amycolatopsis cihanbeyliensis OX=1128664 GN=FB471_3812 PE=4 SV=1

MM1 pKa = 7.63SKK3 pKa = 10.5GKK5 pKa = 8.53HH6 pKa = 4.4TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84THH19 pKa = 5.69GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVATRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.66GRR42 pKa = 11.84ARR44 pKa = 11.84LSAA47 pKa = 3.91

Molecular weight: 5.55 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
6558	0	6558	2202454	29	7822	335.8	36.07

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
13.161 ± 0.051	0.808 ± 0.008	5.762 ± 0.022	6.197 ± 0.03	2.779 ± 0.018	9.515 ± 0.03	2.254 ± 0.015	3.3 ± 0.022	1.721 ± 0.019	10.718 ± 0.039

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.682 ± 0.012	1.846 ± 0.014	6.004 ± 0.027	2.747 ± 0.017	8.412 ± 0.031	4.962 ± 0.022	5.985 ± 0.025	8.587 ± 0.029	1.521 ± 0.01	2.04 ± 0.015

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski