Amino acid dipepetide frequency for Methanosaeta harundinacea (strain 6Ac)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.643AlaAla: 10.643 ± 0.2
0.94AlaCys: 0.94 ± 0.037
5.051AlaAsp: 5.051 ± 0.088
8.236AlaGlu: 8.236 ± 0.151
3.285AlaPhe: 3.285 ± 0.077
8.954AlaGly: 8.954 ± 0.129
1.223AlaHis: 1.223 ± 0.049
5.511AlaIle: 5.511 ± 0.118
3.714AlaLys: 3.714 ± 0.082
9.793AlaLeu: 9.793 ± 0.197
2.204AlaMet: 2.204 ± 0.065
1.984AlaAsn: 1.984 ± 0.069
3.587AlaPro: 3.587 ± 0.087
1.569AlaGln: 1.569 ± 0.05
6.172AlaArg: 6.172 ± 0.113
5.754AlaSer: 5.754 ± 0.106
4.103AlaThr: 4.103 ± 0.088
7.557AlaVal: 7.557 ± 0.122
1.049AlaTrp: 1.049 ± 0.05
2.111AlaTyr: 2.111 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.726CysAla: 0.726 ± 0.032
0.222CysCys: 0.222 ± 0.02
0.711CysAsp: 0.711 ± 0.036
0.7CysGlu: 0.7 ± 0.042
0.335CysPhe: 0.335 ± 0.022
1.438CysGly: 1.438 ± 0.052
0.249CysHis: 0.249 ± 0.019
0.571CysIle: 0.571 ± 0.033
0.378CysLys: 0.378 ± 0.026
0.858CysLeu: 0.858 ± 0.036
0.218CysMet: 0.218 ± 0.019
0.302CysAsn: 0.302 ± 0.02
0.821CysPro: 0.821 ± 0.041
0.238CysGln: 0.238 ± 0.017
0.772CysArg: 0.772 ± 0.033
0.672CysSer: 0.672 ± 0.035
0.599CysThr: 0.599 ± 0.033
0.636CysVal: 0.636 ± 0.032
0.107CysTrp: 0.107 ± 0.01
0.386CysTyr: 0.386 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
4.225AspAla: 4.225 ± 0.08
0.562AspCys: 0.562 ± 0.035
2.926AspAsp: 2.926 ± 0.083
4.513AspGlu: 4.513 ± 0.091
2.238AspPhe: 2.238 ± 0.064
5.704AspGly: 5.704 ± 0.12
1.142AspHis: 1.142 ± 0.042
2.718AspIle: 2.718 ± 0.072
1.762AspLys: 1.762 ± 0.066
8.202AspLeu: 8.202 ± 0.117
1.237AspMet: 1.237 ± 0.041
1.149AspAsn: 1.149 ± 0.062
4.311AspPro: 4.311 ± 0.109
1.421AspGln: 1.421 ± 0.05
4.441AspArg: 4.441 ± 0.096
2.717AspSer: 2.717 ± 0.066
1.688AspThr: 1.688 ± 0.056
4.125AspVal: 4.125 ± 0.077
0.783AspTrp: 0.783 ± 0.039
1.918AspTyr: 1.918 ± 0.062
0.0AspXaa: 0.0 ± 0.0
Glu
9.02GluAla: 9.02 ± 0.17
0.576GluCys: 0.576 ± 0.029
4.342GluAsp: 4.342 ± 0.086
8.53GluGlu: 8.53 ± 0.147
2.495GluPhe: 2.495 ± 0.066
7.339GluGly: 7.339 ± 0.113
0.973GluHis: 0.973 ± 0.037
6.074GluIle: 6.074 ± 0.107
3.716GluLys: 3.716 ± 0.087
6.299GluLeu: 6.299 ± 0.106
2.718GluMet: 2.718 ± 0.06
2.174GluAsn: 2.174 ± 0.09
2.735GluPro: 2.735 ± 0.065
1.062GluGln: 1.062 ± 0.046
6.152GluArg: 6.152 ± 0.14
3.39GluSer: 3.39 ± 0.068
3.426GluThr: 3.426 ± 0.077
7.296GluVal: 7.296 ± 0.116
0.7GluTrp: 0.7 ± 0.033
1.922GluTyr: 1.922 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
3.007PheAla: 3.007 ± 0.065
0.477PheCys: 0.477 ± 0.032
2.556PheAsp: 2.556 ± 0.065
2.772PheGlu: 2.772 ± 0.07
1.379PhePhe: 1.379 ± 0.05
3.582PheGly: 3.582 ± 0.096
0.497PheHis: 0.497 ± 0.026
1.941PheIle: 1.941 ± 0.063
1.331PheLys: 1.331 ± 0.048
3.367PheLeu: 3.367 ± 0.071
0.77PheMet: 0.77 ± 0.036
1.115PheAsn: 1.115 ± 0.055
1.423PhePro: 1.423 ± 0.047
0.835PheGln: 0.835 ± 0.038
2.209PheArg: 2.209 ± 0.054
2.61PheSer: 2.61 ± 0.064
1.673PheThr: 1.673 ± 0.055
2.751PheVal: 2.751 ± 0.059
0.486PheTrp: 0.486 ± 0.032
1.211PheTyr: 1.211 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
7.622GlyAla: 7.622 ± 0.133
1.305GlyCys: 1.305 ± 0.044
5.42GlyAsp: 5.42 ± 0.103
7.373GlyGlu: 7.373 ± 0.121
3.221GlyPhe: 3.221 ± 0.066
8.579GlyGly: 8.579 ± 0.162
1.423GlyHis: 1.423 ± 0.045
5.645GlyIle: 5.645 ± 0.103
3.766GlyLys: 3.766 ± 0.089
7.869GlyLeu: 7.869 ± 0.118
2.604GlyMet: 2.604 ± 0.061
2.277GlyAsn: 2.277 ± 0.088
3.623GlyPro: 3.623 ± 0.081
1.573GlyGln: 1.573 ± 0.05
7.031GlyArg: 7.031 ± 0.118
6.185GlySer: 6.185 ± 0.106
4.398GlyThr: 4.398 ± 0.105
6.831GlyVal: 6.831 ± 0.115
1.404GlyTrp: 1.404 ± 0.065
2.948GlyTyr: 2.948 ± 0.075
0.0GlyXaa: 0.0 ± 0.0
His
1.163HisAla: 1.163 ± 0.044
0.219HisCys: 0.219 ± 0.016
0.822HisAsp: 0.822 ± 0.033
0.761HisGlu: 0.761 ± 0.04
0.636HisPhe: 0.636 ± 0.028
1.151HisGly: 1.151 ± 0.041
0.467HisHis: 0.467 ± 0.03
0.836HisIle: 0.836 ± 0.036
0.46HisLys: 0.46 ± 0.024
2.048HisLeu: 2.048 ± 0.055
0.267HisMet: 0.267 ± 0.019
0.416HisAsn: 0.416 ± 0.025
1.291HisPro: 1.291 ± 0.047
0.617HisGln: 0.617 ± 0.027
1.209HisArg: 1.209 ± 0.04
0.862HisSer: 0.862 ± 0.04
0.617HisThr: 0.617 ± 0.03
0.982HisVal: 0.982 ± 0.037
0.167HisTrp: 0.167 ± 0.015
0.461HisTyr: 0.461 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.296IleAla: 5.296 ± 0.093
0.743IleCys: 0.743 ± 0.029
4.069IleAsp: 4.069 ± 0.078
4.652IleGlu: 4.652 ± 0.076
2.635IlePhe: 2.635 ± 0.07
4.872IleGly: 4.872 ± 0.08
0.884IleHis: 0.884 ± 0.037
3.106IleIle: 3.106 ± 0.083
2.474IleLys: 2.474 ± 0.073
6.196IleLeu: 6.196 ± 0.115
1.223IleMet: 1.223 ± 0.047
1.74IleAsn: 1.74 ± 0.055
2.878IlePro: 2.878 ± 0.061
1.335IleGln: 1.335 ± 0.046
3.983IleArg: 3.983 ± 0.083
4.658IleSer: 4.658 ± 0.078
2.873IleThr: 2.873 ± 0.067
4.467IleVal: 4.467 ± 0.09
0.664IleTrp: 0.664 ± 0.033
1.917IleTyr: 1.917 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
3.702LysAla: 3.702 ± 0.077
0.346LysCys: 0.346 ± 0.026
2.353LysAsp: 2.353 ± 0.067
3.396LysGlu: 3.396 ± 0.079
1.264LysPhe: 1.264 ± 0.057
3.371LysGly: 3.371 ± 0.087
0.479LysHis: 0.479 ± 0.028
3.054LysIle: 3.054 ± 0.065
2.294LysLys: 2.294 ± 0.075
3.141LysLeu: 3.141 ± 0.078
1.311LysMet: 1.311 ± 0.048
1.606LysAsn: 1.606 ± 0.049
1.684LysPro: 1.684 ± 0.056
0.658LysGln: 0.658 ± 0.04
2.972LysArg: 2.972 ± 0.076
2.152LysSer: 2.152 ± 0.057
2.242LysThr: 2.242 ± 0.067
3.5LysVal: 3.5 ± 0.083
0.386LysTrp: 0.386 ± 0.024
1.198LysTyr: 1.198 ± 0.046
0.0LysXaa: 0.0 ± 0.0
Leu
10.474LeuAla: 10.474 ± 0.15
0.989LeuCys: 0.989 ± 0.049
5.694LeuAsp: 5.694 ± 0.093
7.752LeuGlu: 7.752 ± 0.124
3.479LeuPhe: 3.479 ± 0.081
8.2LeuGly: 8.2 ± 0.12
1.446LeuHis: 1.446 ± 0.043
5.474LeuIle: 5.474 ± 0.102
4.426LeuLys: 4.426 ± 0.083
9.995LeuLeu: 9.995 ± 0.192
2.207LeuMet: 2.207 ± 0.062
2.536LeuAsn: 2.536 ± 0.068
4.374LeuPro: 4.374 ± 0.079
2.071LeuGln: 2.071 ± 0.057
6.466LeuArg: 6.466 ± 0.118
6.73LeuSer: 6.73 ± 0.099
4.059LeuThr: 4.059 ± 0.085
7.094LeuVal: 7.094 ± 0.113
1.045LeuTrp: 1.045 ± 0.044
2.454LeuTyr: 2.454 ± 0.066
0.0LeuXaa: 0.0 ± 0.0
Met
2.959MetAla: 2.959 ± 0.062
0.109MetCys: 0.109 ± 0.012
1.446MetAsp: 1.446 ± 0.053
2.148MetGlu: 2.148 ± 0.057
0.438MetPhe: 0.438 ± 0.027
2.338MetGly: 2.338 ± 0.06
0.268MetHis: 0.268 ± 0.021
1.772MetIle: 1.772 ± 0.06
1.597MetLys: 1.597 ± 0.048
1.681MetLeu: 1.681 ± 0.054
0.798MetMet: 0.798 ± 0.034
0.841MetAsn: 0.841 ± 0.04
1.078MetPro: 1.078 ± 0.035
0.378MetGln: 0.378 ± 0.026
1.773MetArg: 1.773 ± 0.048
1.089MetSer: 1.089 ± 0.043
1.53MetThr: 1.53 ± 0.053
2.011MetVal: 2.011 ± 0.062
0.123MetTrp: 0.123 ± 0.012
0.472MetTyr: 0.472 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
2.107AsnAla: 2.107 ± 0.069
0.352AsnCys: 0.352 ± 0.026
1.282AsnAsp: 1.282 ± 0.056
1.493AsnGlu: 1.493 ± 0.061
1.222AsnPhe: 1.222 ± 0.059
2.097AsnGly: 2.097 ± 0.069
0.436AsnHis: 0.436 ± 0.024
1.647AsnIle: 1.647 ± 0.063
1.074AsnLys: 1.074 ± 0.056
3.117AsnLeu: 3.117 ± 0.084
0.547AsnMet: 0.547 ± 0.027
1.063AsnAsn: 1.063 ± 0.083
1.788AsnPro: 1.788 ± 0.056
0.666AsnGln: 0.666 ± 0.03
1.835AsnArg: 1.835 ± 0.052
1.586AsnSer: 1.586 ± 0.063
1.136AsnThr: 1.136 ± 0.056
2.396AsnVal: 2.396 ± 0.112
0.395AsnTrp: 0.395 ± 0.028
0.977AsnTyr: 0.977 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
4.326ProAla: 4.326 ± 0.085
0.495ProCys: 0.495 ± 0.027
2.925ProAsp: 2.925 ± 0.079
4.913ProGlu: 4.913 ± 0.087
1.757ProPhe: 1.757 ± 0.046
4.799ProGly: 4.799 ± 0.11
0.815ProHis: 0.815 ± 0.034
2.612ProIle: 2.612 ± 0.059
1.846ProLys: 1.846 ± 0.055
4.438ProLeu: 4.438 ± 0.088
1.06ProMet: 1.06 ± 0.038
1.166ProAsn: 1.166 ± 0.045
2.794ProPro: 2.794 ± 0.086
1.163ProGln: 1.163 ± 0.041
2.773ProArg: 2.773 ± 0.066
3.111ProSer: 3.111 ± 0.082
2.145ProThr: 2.145 ± 0.055
3.396ProVal: 3.396 ± 0.082
0.579ProTrp: 0.579 ± 0.028
1.23ProTyr: 1.23 ± 0.051
0.0ProXaa: 0.0 ± 0.0
Gln
1.854GlnAla: 1.854 ± 0.06
0.171GlnCys: 0.171 ± 0.015
1.181GlnAsp: 1.181 ± 0.048
1.85GlnGlu: 1.85 ± 0.059
0.698GlnPhe: 0.698 ± 0.027
1.606GlnGly: 1.606 ± 0.049
0.317GlnHis: 0.317 ± 0.024
1.536GlnIle: 1.536 ± 0.044
1.055GlnLys: 1.055 ± 0.039
1.714GlnLeu: 1.714 ± 0.051
0.484GlnMet: 0.484 ± 0.029
0.725GlnAsn: 0.725 ± 0.035
0.837GlnPro: 0.837 ± 0.034
0.513GlnGln: 0.513 ± 0.031
1.546GlnArg: 1.546 ± 0.043
1.014GlnSer: 1.014 ± 0.038
0.965GlnThr: 0.965 ± 0.04
1.636GlnVal: 1.636 ± 0.049
0.227GlnTrp: 0.227 ± 0.02
0.55GlnTyr: 0.55 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
6.124ArgAla: 6.124 ± 0.115
0.861ArgCys: 0.861 ± 0.037
3.877ArgAsp: 3.877 ± 0.076
5.623ArgGlu: 5.623 ± 0.114
2.382ArgPhe: 2.382 ± 0.067
6.404ArgGly: 6.404 ± 0.12
1.04ArgHis: 1.04 ± 0.042
4.554ArgIle: 4.554 ± 0.082
2.562ArgLys: 2.562 ± 0.062
6.783ArgLeu: 6.783 ± 0.126
2.126ArgMet: 2.126 ± 0.061
1.612ArgAsn: 1.612 ± 0.052
3.195ArgPro: 3.195 ± 0.075
1.311ArgGln: 1.311 ± 0.046
5.913ArgArg: 5.913 ± 0.132
4.423ArgSer: 4.423 ± 0.091
2.861ArgThr: 2.861 ± 0.072
4.881ArgVal: 4.881 ± 0.09
0.933ArgTrp: 0.933 ± 0.038
2.197ArgTyr: 2.197 ± 0.061
0.0ArgXaa: 0.0 ± 0.0
Ser
5.392SerAla: 5.392 ± 0.092
0.72SerCys: 0.72 ± 0.033
3.374SerAsp: 3.374 ± 0.067
4.36SerGlu: 4.36 ± 0.079
2.542SerPhe: 2.542 ± 0.075
6.463SerGly: 6.463 ± 0.116
0.921SerHis: 0.921 ± 0.033
3.838SerIle: 3.838 ± 0.08
2.506SerLys: 2.506 ± 0.062
5.723SerLeu: 5.723 ± 0.11
1.465SerMet: 1.465 ± 0.042
1.587SerAsn: 1.587 ± 0.055
3.513SerPro: 3.513 ± 0.075
1.387SerGln: 1.387 ± 0.048
4.018SerArg: 4.018 ± 0.092
4.33SerSer: 4.33 ± 0.106
2.764SerThr: 2.764 ± 0.068
4.004SerVal: 4.004 ± 0.082
0.893SerTrp: 0.893 ± 0.044
1.906SerTyr: 1.906 ± 0.056
0.0SerXaa: 0.0 ± 0.0
Thr
4.136ThrAla: 4.136 ± 0.097
0.527ThrCys: 0.527 ± 0.034
2.442ThrAsp: 2.442 ± 0.069
2.833ThrGlu: 2.833 ± 0.07
1.799ThrPhe: 1.799 ± 0.057
4.519ThrGly: 4.519 ± 0.092
0.739ThrHis: 0.739 ± 0.036
3.106ThrIle: 3.106 ± 0.084
1.72ThrLys: 1.72 ± 0.057
4.489ThrLeu: 4.489 ± 0.099
0.895ThrMet: 0.895 ± 0.038
1.419ThrAsn: 1.419 ± 0.071
2.617ThrPro: 2.617 ± 0.072
0.691ThrGln: 0.691 ± 0.034
2.5ThrArg: 2.5 ± 0.059
2.859ThrSer: 2.859 ± 0.08
2.457ThrThr: 2.457 ± 0.073
3.779ThrVal: 3.779 ± 0.092
0.535ThrTrp: 0.535 ± 0.036
1.376ThrTyr: 1.376 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
7.525ValAla: 7.525 ± 0.118
0.852ValCys: 0.852 ± 0.038
4.621ValAsp: 4.621 ± 0.079
6.508ValGlu: 6.508 ± 0.101
2.606ValPhe: 2.606 ± 0.062
6.395ValGly: 6.395 ± 0.11
1.304ValHis: 1.304 ± 0.044
4.352ValIle: 4.352 ± 0.086
2.914ValLys: 2.914 ± 0.073
7.287ValLeu: 7.287 ± 0.105
1.831ValMet: 1.831 ± 0.049
1.937ValAsn: 1.937 ± 0.073
3.698ValPro: 3.698 ± 0.081
1.735ValGln: 1.735 ± 0.05
4.907ValArg: 4.907 ± 0.099
4.658ValSer: 4.658 ± 0.078
3.765ValThr: 3.765 ± 0.143
7.079ValVal: 7.079 ± 0.134
0.867ValTrp: 0.867 ± 0.054
2.259ValTyr: 2.259 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
0.876TrpAla: 0.876 ± 0.039
0.129TrpCys: 0.129 ± 0.016
0.757TrpAsp: 0.757 ± 0.045
0.772TrpGlu: 0.772 ± 0.032
0.401TrpPhe: 0.401 ± 0.027
0.897TrpGly: 0.897 ± 0.044
0.201TrpHis: 0.201 ± 0.02
0.787TrpIle: 0.787 ± 0.032
0.521TrpLys: 0.521 ± 0.031
1.012TrpLeu: 1.012 ± 0.044
0.345TrpMet: 0.345 ± 0.026
0.514TrpAsn: 0.514 ± 0.034
0.471TrpPro: 0.471 ± 0.03
0.371TrpGln: 0.371 ± 0.023
0.915TrpArg: 0.915 ± 0.039
0.832TrpSer: 0.832 ± 0.04
0.776TrpThr: 0.776 ± 0.043
0.688TrpVal: 0.688 ± 0.032
0.216TrpTrp: 0.216 ± 0.019
0.427TrpTyr: 0.427 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.266TyrAla: 2.266 ± 0.06
0.343TyrCys: 0.343 ± 0.024
1.933TyrAsp: 1.933 ± 0.089
1.757TyrGlu: 1.757 ± 0.056
1.181TyrPhe: 1.181 ± 0.048
2.389TyrGly: 2.389 ± 0.058
0.629TyrHis: 0.629 ± 0.031
1.531TyrIle: 1.531 ± 0.05
0.993TyrLys: 0.993 ± 0.042
2.954TyrLeu: 2.954 ± 0.068
0.539TyrMet: 0.539 ± 0.029
1.051TyrAsn: 1.051 ± 0.046
1.624TyrPro: 1.624 ± 0.049
0.885TyrGln: 0.885 ± 0.039
2.075TyrArg: 2.075 ± 0.056
2.073TyrSer: 2.073 ± 0.058
1.36TyrThr: 1.36 ± 0.054
1.991TyrVal: 1.991 ± 0.056
0.347TyrTrp: 0.347 ± 0.025
1.006TyrTyr: 1.006 ± 0.042
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2358 proteins (730938 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski