Kocuria palustris PEL

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Kocuria; Kocuria palustris

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2561 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M2XFH2|M2XFH2_9MICC Probable cell division protein WhiA OS=Kocuria palustris PEL OX=1236550 GN=whiA PE=3 SV=1
MM1 pKa = 7.08KK2 pKa = 10.14ASRR5 pKa = 11.84IGQSAAILSIGAIALTACGGGGTTTLQGAGASSQEE40 pKa = 3.82AAMNAWSDD48 pKa = 3.77GAGEE52 pKa = 4.72LDD54 pKa = 3.6PAVEE58 pKa = 4.18VRR60 pKa = 11.84YY61 pKa = 10.7SPDD64 pKa = 2.76GSGAGRR70 pKa = 11.84EE71 pKa = 3.98AFLAGGASFAGSDD84 pKa = 3.04AAMDD88 pKa = 3.73EE89 pKa = 4.61EE90 pKa = 4.59EE91 pKa = 4.52FEE93 pKa = 4.58SSKK96 pKa = 9.99EE97 pKa = 3.84QCGDD101 pKa = 2.86KK102 pKa = 11.12GAFNVPAYY110 pKa = 9.57ISPIAVGFNLEE121 pKa = 5.02GIDD124 pKa = 4.8SLNLDD129 pKa = 4.06AEE131 pKa = 4.7TIAGIFSGEE140 pKa = 3.54ITTWNDD146 pKa = 2.97PAIAQQNPDD155 pKa = 3.76ADD157 pKa = 4.42LPDD160 pKa = 3.71TNITVVHH167 pKa = 6.74RR168 pKa = 11.84SDD170 pKa = 3.92EE171 pKa = 4.41SGTTEE176 pKa = 3.9NFTDD180 pKa = 4.14YY181 pKa = 11.05LHH183 pKa = 7.17AAAPEE188 pKa = 3.97AWSDD192 pKa = 3.7EE193 pKa = 4.22ASEE196 pKa = 4.28SWPISGQEE204 pKa = 3.96NSQGTSGVVSTASDD218 pKa = 3.29TDD220 pKa = 3.79GAITYY225 pKa = 10.25ADD227 pKa = 3.41ASAIGDD233 pKa = 4.16LGTVAVGVGDD243 pKa = 4.86DD244 pKa = 3.8YY245 pKa = 12.18VEE247 pKa = 4.22YY248 pKa = 11.08SPDD251 pKa = 3.18AAAAAVDD258 pKa = 3.66ASQRR262 pKa = 11.84VEE264 pKa = 4.76GGAEE268 pKa = 3.69NNMALEE274 pKa = 4.57LDD276 pKa = 4.19RR277 pKa = 11.84DD278 pKa = 4.16TEE280 pKa = 4.31ADD282 pKa = 3.19GAYY285 pKa = 9.73PIILVSYY292 pKa = 9.89HH293 pKa = 6.82IYY295 pKa = 10.27CNEE298 pKa = 3.9YY299 pKa = 10.2QDD301 pKa = 5.15QDD303 pKa = 3.56TADD306 pKa = 3.33AAKK309 pKa = 10.76AFGQYY314 pKa = 9.14VVSEE318 pKa = 4.39EE319 pKa = 4.25GQQASADD326 pKa = 3.97AAHH329 pKa = 6.55SAPLSEE335 pKa = 4.83KK336 pKa = 9.48MRR338 pKa = 11.84QEE340 pKa = 3.45AHH342 pKa = 6.94DD343 pKa = 5.57AIDD346 pKa = 3.58QITAAGG352 pKa = 3.45

Molecular weight: 36.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M2XD66|M2XD66_9MICC Regulatory protein ArsR family OS=Kocuria palustris PEL OX=1236550 GN=C884_02162 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84GILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.38GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2561

0

2561

840818

37

1693

328.3

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.598 ± 0.068

0.632 ± 0.012

6.191 ± 0.043

6.66 ± 0.049

2.787 ± 0.031

9.013 ± 0.045

2.163 ± 0.023

4.119 ± 0.035

1.912 ± 0.035

10.099 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.021

1.667 ± 0.022

5.568 ± 0.037

3.752 ± 0.028

7.624 ± 0.053

6.182 ± 0.035

5.487 ± 0.03

8.15 ± 0.041

1.479 ± 0.019

1.759 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski