Lactobacillus virus Lb338-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Mooreparkvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 199 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C1KFD7|C1KFD7_9CAUD Uncharacterized protein OS=Lactobacillus virus Lb338-1 OX=632112 GN=lb338_phage_27 PE=4 SV=1
MM1 pKa = 7.23SHH3 pKa = 7.34KK4 pKa = 10.27LTDD7 pKa = 3.72NEE9 pKa = 4.16HH10 pKa = 7.34DD11 pKa = 3.93SLKK14 pKa = 11.0LSVLEE19 pKa = 4.39QAGVDD24 pKa = 3.29NWIGYY29 pKa = 7.77SAVGAVYY36 pKa = 10.09DD37 pKa = 4.22YY38 pKa = 11.66EE39 pKa = 6.16DD40 pKa = 3.87YY41 pKa = 11.23LYY43 pKa = 11.35GLDD46 pKa = 5.51DD47 pKa = 4.81DD48 pKa = 4.17QTPLSFYY55 pKa = 11.01EE56 pKa = 4.15WLDD59 pKa = 3.65KK60 pKa = 11.24NNKK63 pKa = 10.13SIDD66 pKa = 3.38DD67 pKa = 3.82WLL69 pKa = 5.72

Molecular weight:
8.02 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C1KFF9|C1KFF9_9CAUD Transposase OS=Lactobacillus virus Lb338-1 OX=632112 GN=lb338_phage_49 PE=3 SV=1
MM1 pKa = 7.55GSASYY6 pKa = 11.06CDD8 pKa = 3.1IRR10 pKa = 11.84HH11 pKa = 4.76RR12 pKa = 11.84TGWVGNRR19 pKa = 11.84RR20 pKa = 11.84PVGNKK25 pKa = 8.38PASPCGSSWGNTGTLQLPP43 pKa = 4.17

Molecular weight:
4.57 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

199

0

199

42470

32

2222

213.4

24.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.388 ± 0.427

0.716 ± 0.082

6.981 ± 0.187

5.745 ± 0.276

3.563 ± 0.125

6.113 ± 0.198

1.754 ± 0.102

6.313 ± 0.154

8.585 ± 0.381

8.208 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.428 ± 0.107

5.978 ± 0.172

3.212 ± 0.115

3.786 ± 0.178

3.52 ± 0.167

7.725 ± 0.289

6.11 ± 0.387

6.442 ± 0.144

1.187 ± 0.069

5.246 ± 0.139

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski