Psychrobacillus sp. FJAT-21963

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Psychrobacillus; unclassified Psychrobacillus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3910 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q3W266|A0A0Q3W266_9BACI Flagellar motor switch protein FliM OS=Psychrobacillus sp. FJAT-21963 OX=1712028 GN=AN959_06355 PE=3 SV=1
MM1 pKa = 7.25SVCNGCQKK9 pKa = 10.73SFEE12 pKa = 4.19EE13 pKa = 4.34SLLITTDD20 pKa = 3.05HH21 pKa = 6.44FRR23 pKa = 11.84GEE25 pKa = 4.31ALVQYY30 pKa = 10.67CDD32 pKa = 3.24VCFLEE37 pKa = 4.8GARR40 pKa = 11.84DD41 pKa = 3.84GFGDD45 pKa = 4.11KK46 pKa = 10.55SLQCEE51 pKa = 4.48CGEE54 pKa = 5.29KK55 pKa = 9.91MILDD59 pKa = 3.65QDD61 pKa = 4.08DD62 pKa = 4.46EE63 pKa = 4.76EE64 pKa = 4.6VLSLAKK70 pKa = 10.23DD71 pKa = 3.42QEE73 pKa = 4.73VIFYY77 pKa = 9.74RR78 pKa = 11.84CKK80 pKa = 10.24KK81 pKa = 9.59VVEE84 pKa = 3.8ARR86 pKa = 11.84LAKK89 pKa = 10.64NYY91 pKa = 10.81DD92 pKa = 3.5LVEE95 pKa = 4.1QMEE98 pKa = 5.01DD99 pKa = 2.85EE100 pKa = 4.68HH101 pKa = 7.85EE102 pKa = 4.25WVGLYY107 pKa = 10.05VIQPEE112 pKa = 4.23DD113 pKa = 3.76DD114 pKa = 3.87YY115 pKa = 11.92EE116 pKa = 4.02

Molecular weight: 13.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q3RY22|A0A0Q3RY22_9BACI Iron ABC transporter substrate-binding protein OS=Psychrobacillus sp. FJAT-21963 OX=1712028 GN=AN959_10930 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.67RR12 pKa = 11.84AKK14 pKa = 9.79NHH16 pKa = 4.64GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.66NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3910

0

3910

1109097

37

1712

283.7

31.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.815 ± 0.047

0.644 ± 0.01

4.974 ± 0.032

7.698 ± 0.054

4.701 ± 0.041

6.639 ± 0.04

1.973 ± 0.019

8.355 ± 0.042

6.927 ± 0.037

9.789 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.019

4.734 ± 0.03

3.461 ± 0.026

3.575 ± 0.026

3.68 ± 0.028

6.221 ± 0.028

5.581 ± 0.025

7.028 ± 0.033

0.993 ± 0.016

3.487 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski