Polaribacter sp. ALD11

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter; unclassified Polaribacter

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2976 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8XMY1|A0A2K8XMY1_9FLAO Uncharacterized protein OS=Polaribacter sp. ALD11 OX=2058137 GN=CW731_02915 PE=4 SV=1
MM1 pKa = 7.75SYY3 pKa = 10.41QAVVRR8 pKa = 11.84DD9 pKa = 3.75ASNTLVSTQAVGMQISILQGSTPVYY34 pKa = 10.86VEE36 pKa = 4.06AQTPTTNANGLVSLEE51 pKa = 4.2IGTGTLLSGDD61 pKa = 3.6FTTINWANDD70 pKa = 2.81AYY72 pKa = 10.63FIKK75 pKa = 10.09TEE77 pKa = 3.98TDD79 pKa = 3.06PTGGTNYY86 pKa = 9.61TITVTTQLMSVPYY99 pKa = 9.97ALHH102 pKa = 6.88AKK104 pKa = 7.68TAEE107 pKa = 4.52SITGTINYY115 pKa = 8.01TEE117 pKa = 4.12TDD119 pKa = 3.72SVFRR123 pKa = 11.84ASIANTITAIDD134 pKa = 3.51TTNWNNHH141 pKa = 4.45TVDD144 pKa = 3.64TDD146 pKa = 3.34THH148 pKa = 6.72IDD150 pKa = 3.48STGVANLGFNTGAIITEE167 pKa = 3.91TDD169 pKa = 3.92PIFRR173 pKa = 11.84ASIANGITAIDD184 pKa = 3.44TANWNNHH191 pKa = 4.31TVDD194 pKa = 3.64TDD196 pKa = 3.34THH198 pKa = 6.72IDD200 pKa = 3.48STGVANLGFNAGGIITEE217 pKa = 4.08TDD219 pKa = 3.31PVFRR223 pKa = 11.84ASITNGITAIDD234 pKa = 3.64TVNWNNKK241 pKa = 9.13LSTEE245 pKa = 3.85IDD247 pKa = 3.36GSVTNEE253 pKa = 3.78IQVLTISNDD262 pKa = 3.3TLFLSKK268 pKa = 10.85GGLVKK273 pKa = 10.66LPAAAVVFSGNYY285 pKa = 10.04ADD287 pKa = 4.5LTNIPAYY294 pKa = 9.85IADD297 pKa = 4.32GDD299 pKa = 5.1DD300 pKa = 3.32NTQLNEE306 pKa = 4.11TQVDD310 pKa = 3.57AFVANNGYY318 pKa = 8.25LTTEE322 pKa = 3.75VDD324 pKa = 3.58GSVTNEE330 pKa = 3.51IEE332 pKa = 4.27LPTGGTNGQVLKK344 pKa = 10.17TDD346 pKa = 3.32GGGNYY351 pKa = 10.22AWVNQTTDD359 pKa = 2.97TDD361 pKa = 3.92TNTQLNEE368 pKa = 4.07TQVDD372 pKa = 3.57AFVANNGYY380 pKa = 8.25LTTEE384 pKa = 3.75VDD386 pKa = 3.58GSVTNEE392 pKa = 3.51IEE394 pKa = 4.27LPTGGTNGQVLKK406 pKa = 10.17TDD408 pKa = 3.32GGGNYY413 pKa = 10.22AWVNQTTDD421 pKa = 2.97TDD423 pKa = 3.92TNTQLNEE430 pKa = 4.07TQVDD434 pKa = 3.57AFVANNGYY442 pKa = 8.25LTTEE446 pKa = 3.75VDD448 pKa = 3.58GSVTNEE454 pKa = 3.51IEE456 pKa = 4.27LPTGGTNGQVLKK468 pKa = 10.17TDD470 pKa = 3.58GSGNYY475 pKa = 9.82AWVNQTTDD483 pKa = 3.11TDD485 pKa = 3.3TDD487 pKa = 3.69TQLNEE492 pKa = 4.11TQVDD496 pKa = 3.57AFVANNGYY504 pKa = 8.25LTTEE508 pKa = 3.75VDD510 pKa = 3.58GSVTNEE516 pKa = 3.51IEE518 pKa = 4.27LPTGGTNGQVLKK530 pKa = 10.17TDD532 pKa = 3.58GSGNYY537 pKa = 9.82AWVNQTTDD545 pKa = 3.01TDD547 pKa = 3.8TDD549 pKa = 3.49THH551 pKa = 7.07IDD553 pKa = 3.36STGIANLGYY562 pKa = 9.89IPGVHH567 pKa = 5.56TVNTDD572 pKa = 2.88AQTLKK577 pKa = 10.8ASQTGDD583 pKa = 3.04TLTINGGNSVLIPGISLFNYY603 pKa = 9.32QPQIQIGEE611 pKa = 4.43FYY613 pKa = 10.87AGGVIFWLDD622 pKa = 3.45GNGGGLVVSIVDD634 pKa = 3.37QSTRR638 pKa = 11.84AQWGCHH644 pKa = 3.95GTHH647 pKa = 7.16LAGAEE652 pKa = 4.16GKK654 pKa = 10.49AIGTGAQNTADD665 pKa = 3.65IEE667 pKa = 4.53AGCTRR672 pKa = 11.84YY673 pKa = 8.4ATAADD678 pKa = 3.98LCANLTLNGYY688 pKa = 9.41NDD690 pKa = 3.65WFLPSIDD697 pKa = 3.85EE698 pKa = 4.2LQAIFDD704 pKa = 3.61NRR706 pKa = 11.84AAINATAISNLGTSLGSYY724 pKa = 8.46YY725 pKa = 9.98WSSTEE730 pKa = 5.15DD731 pKa = 3.32YY732 pKa = 10.78NYY734 pKa = 9.54WALLYY739 pKa = 10.09HH740 pKa = 6.34FRR742 pKa = 11.84AGSVSEE748 pKa = 4.95DD749 pKa = 3.09KK750 pKa = 11.06FEE752 pKa = 4.11KK753 pKa = 10.34HH754 pKa = 5.11GVRR757 pKa = 11.84AVRR760 pKa = 11.84AFF762 pKa = 3.27

Molecular weight: 80.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8XU82|A0A2K8XU82_9FLAO Uncharacterized protein OS=Polaribacter sp. ALD11 OX=2058137 GN=CW731_15010 PE=4 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGFDD22 pKa = 3.92TITTDD27 pKa = 3.73KK28 pKa = 10.91PEE30 pKa = 4.37KK31 pKa = 10.55SLLAPKK37 pKa = 9.91KK38 pKa = 10.38RR39 pKa = 11.84SGGRR43 pKa = 11.84NNQGRR48 pKa = 11.84MTTRR52 pKa = 11.84NIGGGHH58 pKa = 4.58KK59 pKa = 9.49QKK61 pKa = 10.8YY62 pKa = 9.58RR63 pKa = 11.84IIDD66 pKa = 3.85FKK68 pKa = 10.83RR69 pKa = 11.84DD70 pKa = 3.38KK71 pKa = 10.89QGIPATVKK79 pKa = 10.01TIEE82 pKa = 4.0YY83 pKa = 9.86DD84 pKa = 3.47PNRR87 pKa = 11.84TAFIALLSYY96 pKa = 11.49ADD98 pKa = 3.5GEE100 pKa = 4.22KK101 pKa = 10.25RR102 pKa = 11.84YY103 pKa = 10.74VIAQNGLKK111 pKa = 9.81VGQVIVSGEE120 pKa = 4.23GIKK123 pKa = 10.52PEE125 pKa = 4.26IGNGMLLSEE134 pKa = 4.93IPLGTTISCIEE145 pKa = 4.03LRR147 pKa = 11.84PGQGAVMARR156 pKa = 11.84SAGSFAQLLAKK167 pKa = 9.53EE168 pKa = 4.49SKK170 pKa = 9.68YY171 pKa = 10.48ATVKK175 pKa = 10.6LPSGEE180 pKa = 3.84TRR182 pKa = 11.84YY183 pKa = 10.28ILLTCMATIGVVSNSDD199 pKa = 3.21HH200 pKa = 6.09QLLVSGKK207 pKa = 9.49AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84VNAVRR224 pKa = 11.84MNPVDD229 pKa = 3.4HH230 pKa = 7.07PMGGGEE236 pKa = 3.89GRR238 pKa = 11.84ASGGHH243 pKa = 5.13PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.41GFKK256 pKa = 9.24TRR258 pKa = 11.84SKK260 pKa = 9.37TKK262 pKa = 10.52ASNKK266 pKa = 9.94YY267 pKa = 8.99ILEE270 pKa = 4.09RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.2KK274 pKa = 9.94

Molecular weight: 29.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2976

0

2976

1034009

36

5843

347.4

39.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.126 ± 0.042

0.662 ± 0.013

5.31 ± 0.047

6.636 ± 0.047

5.505 ± 0.045

6.168 ± 0.047

1.612 ± 0.022

8.42 ± 0.043

8.798 ± 0.061

9.216 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.022

6.645 ± 0.053

3.065 ± 0.024

3.061 ± 0.023

3.139 ± 0.03

6.61 ± 0.043

6.005 ± 0.063

6.126 ± 0.033

0.974 ± 0.013

3.922 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski