Lactobacillus phage A2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ashduovirus; Lactobacillus virus A2

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8LTA4|Q8LTA4_9CAUD Uncharacterized protein OS=Lactobacillus phage A2 OX=51369 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.24LYY4 pKa = 10.93LVVCEE9 pKa = 4.94TGDD12 pKa = 3.44ADD14 pKa = 3.34QWEE17 pKa = 5.06GGTEE21 pKa = 3.61EE22 pKa = 4.43ADD24 pKa = 3.26AVFATTDD31 pKa = 3.2KK32 pKa = 11.58AKK34 pKa = 10.8LDD36 pKa = 4.57DD37 pKa = 4.06YY38 pKa = 11.36LSTRR42 pKa = 11.84MFSYY46 pKa = 10.93DD47 pKa = 3.19SVITMEE53 pKa = 4.64LDD55 pKa = 2.95KK56 pKa = 10.74EE57 pKa = 4.38YY58 pKa = 10.67PEE60 pKa = 4.34GTKK63 pKa = 10.2SSKK66 pKa = 10.56CLASWWEE73 pKa = 4.28EE74 pKa = 4.51GPCDD78 pKa = 5.41DD79 pKa = 6.08DD80 pKa = 5.84PMDD83 pKa = 3.2II84 pKa = 4.95

Molecular weight: 9.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8LTA3|Q8LTA3_9CAUD Uncharacterized protein OS=Lactobacillus phage A2 OX=51369 PE=4 SV=1
MM1 pKa = 7.15QWTDD5 pKa = 3.4EE6 pKa = 4.28QISGIRR12 pKa = 11.84KK13 pKa = 8.5LASEE17 pKa = 4.46GFTRR21 pKa = 11.84RR22 pKa = 11.84EE23 pKa = 3.87TADD26 pKa = 3.56KK27 pKa = 11.23LGISYY32 pKa = 10.25DD33 pKa = 3.54ALQGKK38 pKa = 8.63ARR40 pKa = 11.84RR41 pKa = 11.84LGIEE45 pKa = 3.63FQKK48 pKa = 10.53PVKK51 pKa = 10.45NEE53 pKa = 3.64YY54 pKa = 10.78DD55 pKa = 3.29SDD57 pKa = 3.98GTQSSEE63 pKa = 4.29TILKK67 pKa = 9.27VVRR70 pKa = 11.84GHH72 pKa = 6.84KK73 pKa = 6.7MTPRR77 pKa = 11.84EE78 pKa = 3.86VWKK81 pKa = 7.95PTGMITPSGSLYY93 pKa = 10.35VPQAIFGSRR102 pKa = 11.84RR103 pKa = 11.84LKK105 pKa = 10.03RR106 pKa = 11.84HH107 pKa = 6.24CIKK110 pKa = 10.65ARR112 pKa = 11.84YY113 pKa = 8.95KK114 pKa = 10.49SGRR117 pKa = 3.51

Molecular weight: 13.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

13124

59

1621

215.1

24.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.412 ± 0.617

0.594 ± 0.142

7.185 ± 0.333

5.669 ± 0.282

3.513 ± 0.173

6.919 ± 0.273

1.836 ± 0.177

5.966 ± 0.202

7.482 ± 0.329

7.848 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.141

4.945 ± 0.263

3.955 ± 0.242

4.069 ± 0.185

4.694 ± 0.334

6.675 ± 0.299

6.233 ± 0.373

6.279 ± 0.206

1.577 ± 0.15

3.574 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski