Pseudogymnoascus sp. 23342-1-I1

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10680 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B8E7C7|A0A1B8E7C7_9PEZI GDP-mannose transporter OS=Pseudogymnoascus sp. 23342-1-I1 OX=1524831 GN=VE03_02658 PE=3 SV=1
MM1 pKa = 7.19QLLNFLPLLLVPAVAAKK18 pKa = 9.04STVYY22 pKa = 10.9LIRR25 pKa = 11.84HH26 pKa = 5.36GEE28 pKa = 3.99KK29 pKa = 10.12PADD32 pKa = 4.0GGDD35 pKa = 3.49GLNAQGLQRR44 pKa = 11.84AQCLRR49 pKa = 11.84NVFGGSSSYY58 pKa = 11.09NIGYY62 pKa = 9.32IMAMTPDD69 pKa = 3.66SDD71 pKa = 3.69GSRR74 pKa = 11.84ARR76 pKa = 11.84PLATVQPLATDD87 pKa = 3.92LGLTVDD93 pKa = 4.21ISCDD97 pKa = 3.54RR98 pKa = 11.84DD99 pKa = 3.73DD100 pKa = 4.24QKK102 pKa = 11.59CVADD106 pKa = 3.81VVNGYY111 pKa = 9.77SGSGNILICWEE122 pKa = 4.27HH123 pKa = 7.23DD124 pKa = 3.36ALTDD128 pKa = 4.04IVDD131 pKa = 3.65EE132 pKa = 5.31LGDD135 pKa = 4.05KK136 pKa = 10.33DD137 pKa = 3.93APSYY141 pKa = 11.0PDD143 pKa = 4.65DD144 pKa = 4.11SFNLIWTDD152 pKa = 3.33PSPYY156 pKa = 10.47SDD158 pKa = 2.72ITATTSEE165 pKa = 4.37NCPGLDD171 pKa = 3.2

Molecular weight: 18.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B8DVZ5|A0A1B8DVZ5_9PEZI WD_REPEATS_REGION domain-containing protein OS=Pseudogymnoascus sp. 23342-1-I1 OX=1524831 GN=VE03_07446 PE=4 SV=1
MM1 pKa = 6.84STSSGPMGVPSAIFGGIPSRR21 pKa = 11.84EE22 pKa = 3.51VDD24 pKa = 3.24IPICAVFIACFVGIGATHH42 pKa = 4.91MTIFQKK48 pKa = 10.29NRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84GHH54 pKa = 5.79KK55 pKa = 9.23FVPNALCFGFSMARR69 pKa = 11.84IATFTMRR76 pKa = 11.84IVWATRR82 pKa = 11.84PTNQNITIAANALVAAGVILLWCINRR108 pKa = 11.84VFATRR113 pKa = 11.84LLGEE117 pKa = 4.01YY118 pKa = 10.07HH119 pKa = 6.58PRR121 pKa = 11.84LYY123 pKa = 10.28TRR125 pKa = 11.84PFFEE129 pKa = 4.14FALRR133 pKa = 11.84RR134 pKa = 11.84LPYY137 pKa = 10.38VVIICCVPMVLVAMVLQIKK156 pKa = 6.18TTNPHH161 pKa = 5.23IRR163 pKa = 11.84VITGILLKK171 pKa = 10.75VVISFFLAFTFSPFIVLAITLLLPGRR197 pKa = 11.84KK198 pKa = 8.85SEE200 pKa = 4.42AEE202 pKa = 4.0VARR205 pKa = 11.84MGKK208 pKa = 10.27GKK210 pKa = 8.92TSTKK214 pKa = 9.98VAIIAIATTLLCWEE228 pKa = 4.54LGIRR232 pKa = 11.84SATMLQKK239 pKa = 10.86LPANAAPWYY248 pKa = 9.29VVASGIEE255 pKa = 4.1LANSMM260 pKa = 4.72

Molecular weight: 28.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10680

0

10680

5193933

51

6772

486.3

53.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.96 ± 0.027

1.148 ± 0.01

5.644 ± 0.017

6.4 ± 0.022

3.631 ± 0.014

7.324 ± 0.021

2.2 ± 0.009

5.011 ± 0.017

4.968 ± 0.023

8.776 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.009

3.673 ± 0.012

5.963 ± 0.027

3.813 ± 0.019

5.869 ± 0.021

8.07 ± 0.027

6.049 ± 0.016

6.187 ± 0.016

1.411 ± 0.008

2.748 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski