Haloarcula hispanica pleomorphic virus 1

Taxonomy: Viruses; Monodnaviria; Trapavirae; Saleviricota; Huolimaviricetes; Haloruvirales; Pleolipoviridae; Alphapleolipovirus; Alphapleolipovirus HHPV1

Average proteome isoelectric point is 5.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3JVC2|D3JVC2_9VIRU ORF5 OS=Haloarcula hispanica pleomorphic virus 1 OX=710112 PE=4 SV=1
MM1 pKa = 7.85SDD3 pKa = 2.84TTMGGSAKK11 pKa = 10.02SSRR14 pKa = 11.84VGRR17 pKa = 11.84RR18 pKa = 11.84TFLKK22 pKa = 9.94TVGVTAGAAAGLHH35 pKa = 5.77HH36 pKa = 7.31TDD38 pKa = 4.06DD39 pKa = 3.87TALDD43 pKa = 3.93PVGEE47 pKa = 4.34ADD49 pKa = 3.31AVVPVVLPGVALAGAAGVGYY69 pKa = 10.48VAGSYY74 pKa = 11.1LEE76 pKa = 5.07DD77 pKa = 3.78NYY79 pKa = 11.35LGDD82 pKa = 3.65SRR84 pKa = 11.84DD85 pKa = 3.52YY86 pKa = 11.3SGYY89 pKa = 9.42TGGDD93 pKa = 3.35ALKK96 pKa = 10.8SAIKK100 pKa = 10.42EE101 pKa = 4.18GATSMKK107 pKa = 10.44VADD110 pKa = 4.56EE111 pKa = 4.45KK112 pKa = 11.68VMSSIQNNIANSQTAGLAKK131 pKa = 10.33GKK133 pKa = 10.24AAVIKK138 pKa = 10.19EE139 pKa = 4.02MNAGNGEE146 pKa = 4.23SAATSAMQSAINEE159 pKa = 4.01YY160 pKa = 10.05FAAIQQNILTHH171 pKa = 5.94YY172 pKa = 10.12NSQLEE177 pKa = 4.36QVKK180 pKa = 9.67HH181 pKa = 6.28HH182 pKa = 6.61VDD184 pKa = 3.34QVANHH189 pKa = 6.57SDD191 pKa = 3.56LTVGDD196 pKa = 3.9VFASEE201 pKa = 4.81NGDD204 pKa = 3.63DD205 pKa = 4.3SPSNMTVNQRR215 pKa = 11.84SLSLVDD221 pKa = 3.65GTDD224 pKa = 3.02ATEE227 pKa = 4.47KK228 pKa = 10.86YY229 pKa = 10.83LIFNTSQDD237 pKa = 2.84GSTYY241 pKa = 10.58EE242 pKa = 4.1SGISLTTATGAADD255 pKa = 3.77FEE257 pKa = 4.75VDD259 pKa = 3.99SFSLSSGAYY268 pKa = 9.71ADD270 pKa = 4.1YY271 pKa = 11.12SDD273 pKa = 3.86EE274 pKa = 4.0FQYY277 pKa = 10.88FQTVPMGNAFDD288 pKa = 4.54AVVTEE293 pKa = 4.1RR294 pKa = 11.84DD295 pKa = 3.48KK296 pKa = 11.79VLDD299 pKa = 4.24DD300 pKa = 4.42LSTFVSDD307 pKa = 4.69VYY309 pKa = 11.0SQYY312 pKa = 11.33SAGDD316 pKa = 3.41IPTEE320 pKa = 4.79DD321 pKa = 3.11IVDD324 pKa = 5.43PITAATEE331 pKa = 3.59LRR333 pKa = 11.84TNYY336 pKa = 10.73DD337 pKa = 3.19GMQGASAHH345 pKa = 6.03AAMLGIPTNADD356 pKa = 2.78QSVYY360 pKa = 7.91MTLEE364 pKa = 4.73DD365 pKa = 4.35DD366 pKa = 5.0GKK368 pKa = 11.12SVWADD373 pKa = 2.94VYY375 pKa = 9.78TNKK378 pKa = 10.65KK379 pKa = 8.04ITDD382 pKa = 3.62SNGNEE387 pKa = 3.69VGFQKK392 pKa = 10.12GTTYY396 pKa = 11.31DD397 pKa = 3.43PTTWSEE403 pKa = 3.73PLYY406 pKa = 10.52IAFEE410 pKa = 4.31YY411 pKa = 10.96TEE413 pKa = 4.38TTDD416 pKa = 3.91SDD418 pKa = 4.54GNVLNSTQQEE428 pKa = 4.7SYY430 pKa = 11.07DD431 pKa = 3.8GEE433 pKa = 4.67TTTTEE438 pKa = 3.65YY439 pKa = 11.31SDD441 pKa = 3.65FVEE444 pKa = 4.53VEE446 pKa = 3.95QPFTIKK452 pKa = 10.54EE453 pKa = 3.88ITVDD457 pKa = 3.88GEE459 pKa = 4.46SKK461 pKa = 10.74QSFKK465 pKa = 8.73TTSRR469 pKa = 11.84NAQTADD475 pKa = 3.13VSKK478 pKa = 10.56LQEE481 pKa = 3.96EE482 pKa = 4.89LEE484 pKa = 4.51QIRR487 pKa = 11.84QTQIDD492 pKa = 4.13MQEE495 pKa = 4.06EE496 pKa = 4.26AQSGGGGGFSVDD508 pKa = 3.59SLSLGGIPGEE518 pKa = 4.2GVLLVGVGIAAWLFGNN534 pKa = 4.69

Molecular weight: 56.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3JVC5|D3JVC5_9VIRU ORF8 OS=Haloarcula hispanica pleomorphic virus 1 OX=710112 PE=4 SV=1
MM1 pKa = 7.65SILRR5 pKa = 11.84KK6 pKa = 9.96LIPVGAGGTLVTHH19 pKa = 6.73CASRR23 pKa = 11.84VNIPFVLWWHH33 pKa = 6.65GEE35 pKa = 4.0TGPRR39 pKa = 11.84PVSRR43 pKa = 11.84VDD45 pKa = 3.04ACSLLTTRR53 pKa = 11.84SHH55 pKa = 6.86KK56 pKa = 10.66PNGQYY61 pKa = 11.06NVTGTSRR68 pKa = 11.84RR69 pKa = 11.84AQEE72 pKa = 4.0TPYY75 pKa = 10.77SRR77 pKa = 11.84SQARR81 pKa = 11.84SEE83 pKa = 4.1TGIPRR88 pKa = 11.84AARR91 pKa = 11.84MLARR95 pKa = 11.84PHH97 pKa = 6.37HH98 pKa = 6.08EE99 pKa = 4.15RR100 pKa = 11.84YY101 pKa = 8.28STTSFHH107 pKa = 6.06NQPVAHH113 pKa = 6.43RR114 pKa = 11.84TPPGGPAGGLLSGVLWVGGSLGGYY138 pKa = 8.71PSQARR143 pKa = 11.84TKK145 pKa = 10.33PVAQPNQSSEE155 pKa = 3.6RR156 pKa = 11.84SLFVRR161 pKa = 11.84SRR163 pKa = 11.84TTITQHH169 pKa = 4.57VEE171 pKa = 3.29QRR173 pKa = 11.84RR174 pKa = 11.84PVIIFSEE181 pKa = 4.31QTGSKK186 pKa = 9.8IPLPRR191 pKa = 11.84RR192 pKa = 11.84RR193 pKa = 11.84VIRR196 pKa = 11.84IILRR200 pKa = 11.84CSFFEE205 pKa = 4.15IAA207 pKa = 5.06

Molecular weight: 22.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

2571

119

783

321.4

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.268 ± 0.698

1.439 ± 0.625

8.518 ± 1.024

7.74 ± 0.85

2.839 ± 0.663

7.74 ± 0.568

1.906 ± 0.527

3.073 ± 0.694

2.995 ± 0.352

7.546 ± 1.163

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.867 ± 0.235

2.956 ± 0.56

4.706 ± 0.74

2.8 ± 0.635

6.34 ± 1.194

7.896 ± 0.678

6.884 ± 0.629

8.674 ± 0.502

1.284 ± 0.195

2.528 ± 0.521
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski