Muribaculaceae bacterium Isolate-013 (NCI)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; unclassified Muribaculaceae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1K6G4|A0A4V1K6G4_9BACT CinA family protein OS=Muribaculaceae bacterium Isolate-013 (NCI) OX=2489218 GN=ED551_04685 PE=4 SV=1
MM1 pKa = 7.27VYY3 pKa = 10.25YY4 pKa = 10.58SKK6 pKa = 8.44THH8 pKa = 5.62EE9 pKa = 4.44YY10 pKa = 10.44IRR12 pKa = 11.84MEE14 pKa = 4.07NEE16 pKa = 3.57TEE18 pKa = 4.14GFVGISAFAAKK29 pKa = 10.18QLGNVVYY36 pKa = 10.89VDD38 pKa = 4.14LPEE41 pKa = 4.74EE42 pKa = 4.3GDD44 pKa = 3.66EE45 pKa = 4.3FSQAEE50 pKa = 4.22DD51 pKa = 3.76FGAIEE56 pKa = 4.35SVKK59 pKa = 10.56AASDD63 pKa = 3.87LYY65 pKa = 11.22APVDD69 pKa = 3.69MEE71 pKa = 4.06VTEE74 pKa = 4.32VNEE77 pKa = 4.03ALADD81 pKa = 3.81NPRR84 pKa = 11.84LINDD88 pKa = 4.19DD89 pKa = 3.44PMANYY94 pKa = 10.1LMKK97 pKa = 10.26VTVTDD102 pKa = 3.93SSQLEE107 pKa = 4.12EE108 pKa = 4.76LMDD111 pKa = 3.82EE112 pKa = 4.01AAYY115 pKa = 10.66ADD117 pKa = 4.45FCAASHH123 pKa = 6.43

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q0J8C0|A0A4Q0J8C0_9BACT Succinate CoA transferase OS=Muribaculaceae bacterium Isolate-013 (NCI) OX=2489218 GN=ED551_04420 PE=3 SV=1
MM1 pKa = 7.68ANFFIKK7 pKa = 10.46RR8 pKa = 11.84MMVGGLVSISDD19 pKa = 3.84RR20 pKa = 11.84EE21 pKa = 4.2RR22 pKa = 11.84TSLLRR27 pKa = 11.84PFIGISAKK35 pKa = 9.56LTFISWIANLFKK47 pKa = 11.02RR48 pKa = 11.84NFLKK52 pKa = 10.13TDD54 pKa = 4.54LLLCDD59 pKa = 4.57CGGTALGFFASAGGTGGVVHH79 pKa = 6.71TNAARR84 pKa = 11.84GGGRR88 pKa = 11.84SGLRR92 pKa = 11.84YY93 pKa = 9.31RR94 pKa = 11.84CYY96 pKa = 10.03GKK98 pKa = 10.32CVAVITSLRR107 pKa = 11.84GWPFSFCWW115 pKa = 3.15

Molecular weight: 12.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2762

0

2762

955877

29

6340

346.1

38.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.218 ± 0.063

1.225 ± 0.019

5.927 ± 0.04

6.099 ± 0.047

4.249 ± 0.03

7.24 ± 0.047

1.848 ± 0.018

6.093 ± 0.041

5.035 ± 0.048

8.735 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.019

4.412 ± 0.038

4.355 ± 0.031

3.017 ± 0.029

5.859 ± 0.05

6.293 ± 0.043

5.883 ± 0.046

6.72 ± 0.04

1.226 ± 0.019

3.883 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski