Paenibacillus sp. HB172198

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4162 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8XG64|A0A4P8XG64_9BACL Uncharacterized protein OS=Paenibacillus sp. HB172198 OX=2565926 GN=E6C60_0736 PE=4 SV=1
MM1 pKa = 7.42AVINRR6 pKa = 11.84FDD8 pKa = 3.48TTINGAITFTGNTIGLSKK26 pKa = 10.53QLNTNNQGDD35 pKa = 3.75QDD37 pKa = 4.99AIGAMATPNPLSQVNDD53 pKa = 4.47FPPGTTLNFQEE64 pKa = 4.36NAASAVLQLPAQSTVLYY81 pKa = 10.95AEE83 pKa = 5.36LIWSGSYY90 pKa = 10.25NVAGQDD96 pKa = 3.2LSAFINDD103 pKa = 3.13NVSFTTPAGSFSIPFDD119 pKa = 3.67PATAATIFNVLYY131 pKa = 10.61VRR133 pKa = 11.84SADD136 pKa = 3.48VTALVQAAGAGTYY149 pKa = 8.73TVGDD153 pKa = 3.8IVATTSAINNTTNSGGWTLMVAYY176 pKa = 9.25QNPTLPIRR184 pKa = 11.84NMSIFVASEE193 pKa = 4.02FVSTGSGSTPFNISGFFTPTTGPVNGRR220 pKa = 11.84LQVSSLEE227 pKa = 4.15GDD229 pKa = 3.65SNRR232 pKa = 11.84IGDD235 pKa = 3.86QLLFGPTVGTLTPVSGPNNPIDD257 pKa = 3.88NFFASQINGDD267 pKa = 4.81DD268 pKa = 3.8GTLDD272 pKa = 3.27TSGTFGNLNQPLGINADD289 pKa = 3.72GRR291 pKa = 11.84RR292 pKa = 11.84QGYY295 pKa = 9.9DD296 pKa = 2.5ITNIDD301 pKa = 3.8VSPQLVNAQTDD312 pKa = 3.61AVIQGTTSGDD322 pKa = 3.08AYY324 pKa = 10.83VINGVGLQIDD334 pKa = 3.84VNAPRR339 pKa = 11.84FEE341 pKa = 4.23ALKK344 pKa = 10.53SADD347 pKa = 3.45VSVVSPGDD355 pKa = 3.47VITYY359 pKa = 7.64TVEE362 pKa = 3.5ITNTGTADD370 pKa = 3.19ATVVVMFDD378 pKa = 4.02ASPEE382 pKa = 4.23CTTLVPNSVTVDD394 pKa = 3.55GVPQPGATPANLPLGNIIPGQTVIVQYY421 pKa = 10.25QLVVSCVPPSGQYY434 pKa = 10.07VNEE437 pKa = 4.12ALFEE441 pKa = 4.14FQFQSEE447 pKa = 4.42PGGPILTGFGASNILVIQSLQQYY470 pKa = 8.12YY471 pKa = 10.71AINLAAIALAEE482 pKa = 4.28LSQAHH487 pKa = 6.11IMNSEE492 pKa = 4.02AEE494 pKa = 4.12KK495 pKa = 10.63LQTVLGTASLPVITPSLVNLNQLIQVNSSVEE526 pKa = 3.98ATLEE530 pKa = 3.99TLSAQEE536 pKa = 3.67IVLLQRR542 pKa = 11.84FEE544 pKa = 4.76HH545 pKa = 6.66ILRR548 pKa = 11.84GLSVTPGTT556 pKa = 4.21

Molecular weight: 58.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8XHF3|A0A4P8XHF3_9BACL ATP-dependent Clp protease proteolytic subunit OS=Paenibacillus sp. HB172198 OX=2565926 GN=clpP PE=3 SV=1
MM1 pKa = 7.27GASSSVFRR9 pKa = 11.84VRR11 pKa = 11.84SALNRR16 pKa = 11.84AARR19 pKa = 11.84TSSSVFGVRR28 pKa = 11.84WARR31 pKa = 11.84GRR33 pKa = 11.84TARR36 pKa = 11.84TSSSVFRR43 pKa = 11.84VRR45 pKa = 11.84WALNRR50 pKa = 11.84AARR53 pKa = 11.84VSSSVFRR60 pKa = 11.84TRR62 pKa = 11.84SARR65 pKa = 11.84KK66 pKa = 8.5RR67 pKa = 11.84AAHH70 pKa = 6.32ASSSVFRR77 pKa = 11.84VHH79 pKa = 7.21LLLTRR84 pKa = 11.84RR85 pKa = 11.84CGSSSSVFDD94 pKa = 4.06VRR96 pKa = 11.84WALNRR101 pKa = 11.84AARR104 pKa = 11.84VSSSVFGVQLLQTRR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84GSSSSVFDD128 pKa = 3.22VHH130 pKa = 6.51WALNRR135 pKa = 11.84AASASSSVFGVHH147 pKa = 5.23WALNRR152 pKa = 11.84TARR155 pKa = 11.84VSSSVFGVHH164 pKa = 5.54WARR167 pKa = 11.84GRR169 pKa = 11.84AAGASSSVFRR179 pKa = 11.84VHH181 pKa = 7.39LLQTRR186 pKa = 11.84RR187 pKa = 11.84CGSSSSVFDD196 pKa = 4.06VRR198 pKa = 11.84WALNRR203 pKa = 11.84AARR206 pKa = 11.84VSSSVFRR213 pKa = 11.84VRR215 pKa = 11.84WALNRR220 pKa = 11.84AAGASSSVFRR230 pKa = 11.84VQLLQTCRR238 pKa = 11.84RR239 pKa = 11.84GSSSSVFDD247 pKa = 4.06VRR249 pKa = 11.84WALNRR254 pKa = 11.84VARR257 pKa = 11.84VSSSVFRR264 pKa = 11.84VRR266 pKa = 11.84WARR269 pKa = 11.84GRR271 pKa = 11.84ATGASSSVFRR281 pKa = 11.84TRR283 pKa = 11.84STRR286 pKa = 11.84KK287 pKa = 8.02RR288 pKa = 11.84AARR291 pKa = 11.84ASSSVFRR298 pKa = 11.84TRR300 pKa = 11.84SPRR303 pKa = 11.84KK304 pKa = 8.77RR305 pKa = 11.84ATHH308 pKa = 6.29ASSSVFRR315 pKa = 11.84VRR317 pKa = 11.84WARR320 pKa = 11.84GRR322 pKa = 11.84AAGASSSVFRR332 pKa = 11.84VRR334 pKa = 11.84WARR337 pKa = 11.84GRR339 pKa = 11.84AARR342 pKa = 11.84ASSSVFGVHH351 pKa = 5.95WARR354 pKa = 11.84GPAAGASSSVFRR366 pKa = 11.84TRR368 pKa = 11.84STRR371 pKa = 11.84KK372 pKa = 7.56QAARR376 pKa = 11.84ASSSVFRR383 pKa = 11.84VRR385 pKa = 11.84SARR388 pKa = 11.84KK389 pKa = 8.77RR390 pKa = 11.84DD391 pKa = 3.37AGTSSSVFRR400 pKa = 11.84VHH402 pKa = 7.39LLQTRR407 pKa = 11.84RR408 pKa = 11.84CGSSSSVFDD417 pKa = 4.06VRR419 pKa = 11.84WALNRR424 pKa = 11.84TARR427 pKa = 11.84VSSSVFRR434 pKa = 11.84TRR436 pKa = 11.84STRR439 pKa = 11.84KK440 pKa = 8.02RR441 pKa = 11.84AARR444 pKa = 11.84ASSSVFRR451 pKa = 11.84VRR453 pKa = 11.84SARR456 pKa = 11.84KK457 pKa = 8.77RR458 pKa = 11.84DD459 pKa = 3.37AGTSSSVFRR468 pKa = 11.84LRR470 pKa = 11.84WALNRR475 pKa = 11.84TAHH478 pKa = 6.03TSSSVFRR485 pKa = 11.84TRR487 pKa = 11.84WARR490 pKa = 11.84GRR492 pKa = 11.84AARR495 pKa = 11.84ASSSVFRR502 pKa = 11.84TRR504 pKa = 11.84STRR507 pKa = 11.84KK508 pKa = 7.44RR509 pKa = 11.84TARR512 pKa = 11.84ASNSVFRR519 pKa = 11.84TRR521 pKa = 11.84SARR524 pKa = 11.84KK525 pKa = 7.85RR526 pKa = 11.84AARR529 pKa = 11.84ASSSVFRR536 pKa = 11.84TRR538 pKa = 11.84SPRR541 pKa = 11.84SRR543 pKa = 11.84IPSPTQKK550 pKa = 8.81ITPSPYY556 pKa = 8.79FRR558 pKa = 11.84PRR560 pKa = 11.84VILFTAYY567 pKa = 10.79VLDD570 pKa = 4.04EE571 pKa = 4.28TT572 pKa = 5.17

Molecular weight: 63.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4162

0

4162

1276428

37

2836

306.7

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.23 ± 0.046

0.778 ± 0.011

5.072 ± 0.026

7.203 ± 0.045

3.865 ± 0.027

7.455 ± 0.037

2.267 ± 0.021

6.203 ± 0.033

5.055 ± 0.036

10.189 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.983 ± 0.02

3.492 ± 0.028

4.22 ± 0.025

4.366 ± 0.03

5.25 ± 0.035

6.596 ± 0.031

5.109 ± 0.026

6.962 ± 0.03

1.257 ± 0.016

3.446 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski