Acinetobacter phage AP22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Obolenskvirus; Acinetobacter virus AP22

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2GUF4|I2GUF4_9CAUD Uncharacterized protein OS=Acinetobacter phage AP22 OX=1187128 PE=4 SV=1
MM1 pKa = 7.25AQYY4 pKa = 9.85DD5 pKa = 3.73IPLAQVPNQFFTTSLNGVTWAITLEE30 pKa = 4.08TRR32 pKa = 11.84LNNLYY37 pKa = 10.27ISLSNNNDD45 pKa = 2.72GDD47 pKa = 3.93VLLNRR52 pKa = 11.84ICLNRR57 pKa = 11.84TYY59 pKa = 10.63LGRR62 pKa = 11.84GFIFVDD68 pKa = 2.96IDD70 pKa = 3.79GNSDD74 pKa = 3.53PEE76 pKa = 4.25YY77 pKa = 9.62TGLGTRR83 pKa = 11.84YY84 pKa = 10.14LLIWTDD90 pKa = 3.71EE91 pKa = 4.13VV92 pKa = 3.51

Molecular weight: 10.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2GUD2|I2GUD2_9CAUD DnaJ-class molecular chaperone with C-terminal Zn finger domain putative OS=Acinetobacter phage AP22 OX=1187128 PE=4 SV=1
MM1 pKa = 7.53SNKK4 pKa = 7.83QTWLQSHH11 pKa = 6.93AEE13 pKa = 4.13TLTQNIVGLLIGFIILKK30 pKa = 10.24CFGLSASEE38 pKa = 4.29SVQLQAVIFATSYY51 pKa = 10.74LRR53 pKa = 11.84SFLIRR58 pKa = 11.84RR59 pKa = 11.84FFNRR63 pKa = 11.84FVGSQPP69 pKa = 3.23

Molecular weight: 7.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

14104

37

727

158.5

17.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.955 ± 0.363

1.227 ± 0.154

6.168 ± 0.262

6.686 ± 0.339

4.424 ± 0.235

7.033 ± 0.247

1.652 ± 0.152

6.92 ± 0.251

7.679 ± 0.389

8.26 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.914 ± 0.19

5.885 ± 0.33

3.191 ± 0.151

3.8 ± 0.254

3.701 ± 0.185

6.246 ± 0.231

5.282 ± 0.303

6.573 ± 0.246

1.553 ± 0.112

3.85 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski