Halobacteroides halobius (strain ATCC 35273 / DSM 5150 / MD-1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halobacteroidaceae; Halobacteroides; Halobacteroides halobius

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0K7Z3|L0K7Z3_HALHC 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase OS=Halobacteroides halobius (strain ATCC 35273 / DSM 5150 / MD-1) OX=748449 GN=Halha_0500 PE=4 SV=1
MM1 pKa = 6.85GQLVAGIILIGVIAGAFYY19 pKa = 10.99LIGSKK24 pKa = 10.4LGIMDD29 pKa = 5.11NDD31 pKa = 3.91NSDD34 pKa = 4.75DD35 pKa = 5.21DD36 pKa = 4.83YY37 pKa = 11.91SCHH40 pKa = 6.54

Molecular weight: 4.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0K602|L0K602_HALHC Uncharacterized protein OS=Halobacteroides halobius (strain ATCC 35273 / DSM 5150 / MD-1) OX=748449 GN=Halha_0449 PE=4 SV=1
MM1 pKa = 7.29PTINQLVRR9 pKa = 11.84NGRR12 pKa = 11.84EE13 pKa = 3.75KK14 pKa = 10.85VKK16 pKa = 10.48KK17 pKa = 10.23KK18 pKa = 10.24KK19 pKa = 9.89GARR22 pKa = 11.84ALEE25 pKa = 4.28GAPQKK30 pKa = 10.66RR31 pKa = 11.84GVCTRR36 pKa = 11.84VYY38 pKa = 9.87TATPKK43 pKa = 10.58KK44 pKa = 9.97PNSALRR50 pKa = 11.84KK51 pKa = 7.21VARR54 pKa = 11.84VRR56 pKa = 11.84LTNGKK61 pKa = 9.59EE62 pKa = 3.3ITAYY66 pKa = 9.89IPGIGHH72 pKa = 6.92NLQEE76 pKa = 4.3HH77 pKa = 5.67SVVLVRR83 pKa = 11.84GGRR86 pKa = 11.84VKK88 pKa = 10.79DD89 pKa = 3.79LPGVRR94 pKa = 11.84YY95 pKa = 8.2TIVRR99 pKa = 11.84GALDD103 pKa = 3.48CAGVEE108 pKa = 4.1DD109 pKa = 4.54RR110 pKa = 11.84KK111 pKa = 10.57QGRR114 pKa = 11.84SKK116 pKa = 11.32YY117 pKa = 9.38GVKK120 pKa = 10.4KK121 pKa = 8.68PTEE124 pKa = 3.83

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2452

0

2452

762441

29

2082

310.9

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.667 ± 0.051

0.763 ± 0.02

5.679 ± 0.044

7.645 ± 0.057

3.787 ± 0.038

6.817 ± 0.047

1.459 ± 0.02

8.404 ± 0.047

8.301 ± 0.069

10.287 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.147 ± 0.021

5.165 ± 0.043

3.177 ± 0.029

4.049 ± 0.039

3.749 ± 0.035

5.461 ± 0.034

5.094 ± 0.038

6.973 ± 0.04

0.809 ± 0.019

3.567 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski