Lactobacillus phage phiLdb

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cequinquevirus; Lactobacillus virus phiLdb

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3PCR3|U3PCR3_9CAUD Putative ParB nuclease OS=Lactobacillus phage phiLdb OX=1399942 GN=phiLdb_0001 PE=4 SV=1
MM1 pKa = 7.34TKK3 pKa = 9.99IITAQKK9 pKa = 10.1IDD11 pKa = 3.74DD12 pKa = 4.31NNYY15 pKa = 9.81EE16 pKa = 4.26LYY18 pKa = 11.16LNGWLGDD25 pKa = 3.59QLYY28 pKa = 10.3EE29 pKa = 3.85DD30 pKa = 4.69TYY32 pKa = 11.45FGGLVFKK39 pKa = 10.84FGTWVLVQNTYY50 pKa = 10.76QGTNEE55 pKa = 4.2EE56 pKa = 4.19IDD58 pKa = 3.95YY59 pKa = 8.58QTDD62 pKa = 3.54DD63 pKa = 4.21LDD65 pKa = 4.01EE66 pKa = 4.3VLWTIDD72 pKa = 4.5CEE74 pKa = 5.79VMDD77 pKa = 5.11CDD79 pKa = 4.08NLEE82 pKa = 4.08VFNDD86 pKa = 3.86FLDD89 pKa = 4.02LQQ91 pKa = 4.07

Molecular weight: 10.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3PDU2|U3PDU2_9CAUD Putative DNA replication protein OS=Lactobacillus phage phiLdb OX=1399942 GN=phiLdb_00039 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.5FIIEE6 pKa = 4.04GRR8 pKa = 11.84LDD10 pKa = 3.57GLNTYY15 pKa = 9.14TGACRR20 pKa = 11.84ANRR23 pKa = 11.84YY24 pKa = 7.54GANAMKK30 pKa = 10.23RR31 pKa = 11.84KK32 pKa = 10.13NEE34 pKa = 3.81AKK36 pKa = 10.23VIEE39 pKa = 4.93GIKK42 pKa = 10.25RR43 pKa = 11.84AGLKK47 pKa = 10.1KK48 pKa = 10.27VEE50 pKa = 4.5EE51 pKa = 4.57YY52 pKa = 10.45PLTLHH57 pKa = 6.42IVWYY61 pKa = 9.28EE62 pKa = 3.58PNKK65 pKa = 10.52RR66 pKa = 11.84RR67 pKa = 11.84DD68 pKa = 3.02IDD70 pKa = 4.05NITFATKK77 pKa = 10.13FIQDD81 pKa = 3.86ALVKK85 pKa = 10.68SGILDD90 pKa = 4.32DD91 pKa = 5.85DD92 pKa = 3.88SQKK95 pKa = 11.2YY96 pKa = 9.12IVGLSHH102 pKa = 6.56QVTVDD107 pKa = 3.18RR108 pKa = 11.84KK109 pKa = 8.42NPRR112 pKa = 11.84IEE114 pKa = 3.9VEE116 pKa = 3.95LRR118 pKa = 11.84KK119 pKa = 10.27AEE121 pKa = 4.0

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

10445

44

968

177.0

20.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.625 ± 0.624

0.986 ± 0.145

6.932 ± 0.405

7.238 ± 0.494

3.791 ± 0.18

6.415 ± 0.346

1.379 ± 0.184

7.046 ± 0.289

8.722 ± 0.386

7.659 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.181

6.596 ± 0.244

2.298 ± 0.144

3.293 ± 0.258

4.088 ± 0.256

6.242 ± 0.285

5.84 ± 0.314

6.778 ± 0.233

1.053 ± 0.106

4.404 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski