Sewage-associated circular DNA virus-18

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UH75|A0A0B4UH75_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-18 OX=1592085 PE=3 SV=1
MM1 pKa = 7.76DD2 pKa = 4.92TFPTPNQLILEE13 pKa = 4.41EE14 pKa = 4.95DD15 pKa = 3.44LAEE18 pKa = 4.78PEE20 pKa = 4.14TLEE23 pKa = 4.95APTEE27 pKa = 3.92KK28 pKa = 10.09ATAKK32 pKa = 10.31GFRR35 pKa = 11.84LSGKK39 pKa = 9.4NFTITFPQCDD49 pKa = 3.96VKK51 pKa = 11.25KK52 pKa = 9.68EE53 pKa = 3.9IAVEE57 pKa = 4.2RR58 pKa = 11.84IEE60 pKa = 4.1QKK62 pKa = 10.42FGSEE66 pKa = 3.3IKK68 pKa = 10.57GYY70 pKa = 9.08LVCEE74 pKa = 4.24EE75 pKa = 3.98QHH77 pKa = 7.19KK78 pKa = 11.14DD79 pKa = 3.49GTPHH83 pKa = 5.87LHH85 pKa = 6.43VYY87 pKa = 10.9LSFLNKK93 pKa = 10.06KK94 pKa = 8.48NFKK97 pKa = 9.87CHH99 pKa = 5.79HH100 pKa = 6.53CFNFIGGKK108 pKa = 8.16QGNYY112 pKa = 7.5QVTKK116 pKa = 10.19SVRR119 pKa = 11.84DD120 pKa = 3.32WVTYY124 pKa = 7.31CTKK127 pKa = 10.55GDD129 pKa = 3.51NYY131 pKa = 9.89VAKK134 pKa = 10.31GLDD137 pKa = 3.2VQAIKK142 pKa = 10.79KK143 pKa = 8.07KK144 pKa = 8.09TAPKK148 pKa = 8.27STTVATMLMEE158 pKa = 4.78GKK160 pKa = 9.92SLSEE164 pKa = 4.01INAVDD169 pKa = 3.67PGYY172 pKa = 12.01VMINKK177 pKa = 9.65RR178 pKa = 11.84KK179 pKa = 9.35LEE181 pKa = 4.24EE182 pKa = 3.99YY183 pKa = 9.67EE184 pKa = 3.88SWVTIEE190 pKa = 4.3RR191 pKa = 11.84NKK193 pKa = 10.3KK194 pKa = 10.3SKK196 pKa = 9.15LTWVPPLLDD205 pKa = 4.14GLTDD209 pKa = 3.75ANKK212 pKa = 10.03QICEE216 pKa = 4.51WICSNIRR223 pKa = 11.84QPRR226 pKa = 11.84KK227 pKa = 9.69FKK229 pKa = 10.88APQLFITGPKK239 pKa = 10.04NLGKK243 pKa = 9.26TSLIEE248 pKa = 3.65WLGQYY253 pKa = 10.55LSLYY257 pKa = 9.69HH258 pKa = 6.99IPQTEE263 pKa = 4.07EE264 pKa = 4.55FYY266 pKa = 11.37DD267 pKa = 4.99LYY269 pKa = 10.88TDD271 pKa = 5.35DD272 pKa = 4.22YY273 pKa = 11.01DD274 pKa = 4.94LVVFDD279 pKa = 4.62EE280 pKa = 5.2FKK282 pKa = 10.68GQKK285 pKa = 8.28TIQWMNLFLQGSPMNIRR302 pKa = 11.84KK303 pKa = 9.75KK304 pKa = 10.04GSQYY308 pKa = 10.75MKK310 pKa = 9.84MKK312 pKa = 9.42NLPVIILSNYY322 pKa = 8.2TLGDD326 pKa = 4.39CYY328 pKa = 10.81PKK330 pKa = 10.9ARR332 pKa = 11.84DD333 pKa = 3.66DD334 pKa = 4.05GRR336 pKa = 11.84LEE338 pKa = 3.99TLQARR343 pKa = 11.84LDD345 pKa = 4.02VIEE348 pKa = 4.44VDD350 pKa = 4.5SFIDD354 pKa = 4.32FYY356 pKa = 11.53KK357 pKa = 10.82DD358 pKa = 3.01RR359 pKa = 11.84SDD361 pKa = 3.42ILL363 pKa = 3.7

Molecular weight: 41.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UH75|A0A0B4UH75_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-18 OX=1592085 PE=3 SV=1
MM1 pKa = 8.05RR2 pKa = 11.84GPTKK6 pKa = 10.39RR7 pKa = 11.84NYY9 pKa = 9.68KK10 pKa = 9.98GSSRR14 pKa = 11.84KK15 pKa = 8.98IAAARR20 pKa = 11.84AAVGRR25 pKa = 11.84ANAVLASRR33 pKa = 11.84SSMSSGSYY41 pKa = 10.28GPPASRR47 pKa = 11.84GFYY50 pKa = 9.85GQYY53 pKa = 10.08SLRR56 pKa = 11.84GRR58 pKa = 11.84AEE60 pKa = 4.04LKK62 pKa = 10.67FVDD65 pKa = 3.91ATSTNAAVTTTWQGALINGIAQGADD90 pKa = 3.13FNQRR94 pKa = 11.84IGRR97 pKa = 11.84KK98 pKa = 8.46AQMKK102 pKa = 9.86SVLFNGNFFPGTTAAEE118 pKa = 4.08NASQGVYY125 pKa = 9.97LRR127 pKa = 11.84VVIVYY132 pKa = 9.65DD133 pKa = 3.55SQPNSGTFPGGTDD146 pKa = 3.53FLGANDD152 pKa = 4.78PNTPLNLNNRR162 pKa = 11.84DD163 pKa = 3.55RR164 pKa = 11.84FSILIDD170 pKa = 3.2VRR172 pKa = 11.84KK173 pKa = 10.22QIGSYY178 pKa = 10.32LFNGTPALTAGSPQNAYY195 pKa = 7.38WNKK198 pKa = 9.58YY199 pKa = 8.24KK200 pKa = 10.58KK201 pKa = 10.16CNKK204 pKa = 7.73EE205 pKa = 4.27TIFSGTAATLGSISTGAMYY224 pKa = 10.42IFFVGDD230 pKa = 3.38FNGVGMIDD238 pKa = 4.22FYY240 pKa = 11.41TRR242 pKa = 11.84VRR244 pKa = 11.84YY245 pKa = 8.36TDD247 pKa = 3.09MM248 pKa = 5.78

Molecular weight: 26.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

611

248

363

305.5

34.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.71 ± 2.373

1.473 ± 0.608

5.401 ± 0.777

4.583 ± 1.916

5.237 ± 0.461

8.02 ± 1.858

0.982 ± 0.558

5.728 ± 0.505

8.02 ± 2.036

7.692 ± 1.392

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.291 ± 0.073

5.892 ± 1.005

4.419 ± 0.22

4.255 ± 0.356

4.746 ± 1.198

6.219 ± 1.277

7.201 ± 0.261

5.237 ± 0.003

1.309 ± 0.286

4.583 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski