Cyanophage MED4-117

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4Q547|M4Q547_9CAUD Uncharacterized protein OS=Cyanophage MED4-117 OX=889954 GN=CPWG_00050 PE=4 SV=1
MM1 pKa = 7.74TYY3 pKa = 10.44PSRR6 pKa = 11.84FPSDD10 pKa = 3.54PYY12 pKa = 10.58EE13 pKa = 4.05GQIFYY18 pKa = 10.93DD19 pKa = 3.83ADD21 pKa = 3.47TDD23 pKa = 3.94RR24 pKa = 11.84TFEE27 pKa = 3.96YY28 pKa = 10.23QRR30 pKa = 11.84RR31 pKa = 11.84DD32 pKa = 3.11ILEE35 pKa = 3.75QMINCSKK42 pKa = 10.45EE43 pKa = 3.5VYY45 pKa = 9.6YY46 pKa = 10.29WFDD49 pKa = 2.86ISKK52 pKa = 10.47EE53 pKa = 3.87II54 pKa = 4.82

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4Q3R5|M4Q3R5_9CAUD DUF4055 domain-containing protein OS=Cyanophage MED4-117 OX=889954 GN=CPWG_00019 PE=4 SV=1
MM1 pKa = 7.52APKK4 pKa = 9.77KK5 pKa = 10.31RR6 pKa = 11.84KK7 pKa = 8.25SAKK10 pKa = 10.02SKK12 pKa = 7.92STKK15 pKa = 10.23AATIKK20 pKa = 10.49KK21 pKa = 9.36KK22 pKa = 10.8AEE24 pKa = 3.64NSIYY28 pKa = 10.5TVGDD32 pKa = 3.46LNKK35 pKa = 9.67VYY37 pKa = 10.86ARR39 pKa = 11.84GVAAYY44 pKa = 9.74LSSGSRR50 pKa = 11.84NVSVGAWSMGRR61 pKa = 11.84VSSFISGGGARR72 pKa = 11.84KK73 pKa = 9.71ADD75 pKa = 3.15IDD77 pKa = 3.27IHH79 pKa = 6.09QNRR82 pKa = 11.84KK83 pKa = 9.71KK84 pKa = 10.63NPKK87 pKa = 9.42KK88 pKa = 10.44RR89 pKa = 3.48

Molecular weight: 9.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12146

50

1200

192.8

21.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.283 ± 0.508

0.955 ± 0.144

5.961 ± 0.303

6.224 ± 0.382

4.224 ± 0.251

6.652 ± 0.524

1.408 ± 0.16

6.438 ± 0.23

7.212 ± 0.571

8.011 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.91 ± 0.216

5.467 ± 0.276

3.664 ± 0.314

4.133 ± 0.23

4.314 ± 0.263

6.916 ± 0.398

7.945 ± 0.632

6.348 ± 0.251

0.947 ± 0.123

2.989 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski