Carrot mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6UQC5|B6UQC5_9TOMB RNA-directed RNA polymerase OS=Carrot mottle virus OX=68033 PE=4 SV=2
MM1 pKa = 7.43SFLKK5 pKa = 10.63RR6 pKa = 11.84ILKK9 pKa = 10.14HH10 pKa = 6.43LDD12 pKa = 3.45SQFSPAPGVYY22 pKa = 10.11NRR24 pKa = 11.84DD25 pKa = 3.01VCAEE29 pKa = 4.19LYY31 pKa = 9.13GAEE34 pKa = 3.97WGVLITQEE42 pKa = 3.8RR43 pKa = 11.84MTRR46 pKa = 11.84ALSVEE51 pKa = 3.85HH52 pKa = 6.09EE53 pKa = 3.75RR54 pKa = 11.84WYY56 pKa = 9.42TGRR59 pKa = 11.84VEE61 pKa = 4.17TDD63 pKa = 2.98VYY65 pKa = 11.0IPVAQYY71 pKa = 10.75VDD73 pKa = 3.6PRR75 pKa = 11.84DD76 pKa = 3.8VEE78 pKa = 4.58AEE80 pKa = 3.98PTSQQGNSTGVDD92 pKa = 3.16NPVNNCSGTVPTMPATEE109 pKa = 4.51VIPVDD114 pKa = 3.45GLGGRR119 pKa = 11.84RR120 pKa = 11.84VGVVPAPEE128 pKa = 4.37GPIPCEE134 pKa = 3.83GNVYY138 pKa = 10.52AILRR142 pKa = 11.84MADD145 pKa = 3.35PTADD149 pKa = 3.42ASEE152 pKa = 4.56SSDD155 pKa = 4.65DD156 pKa = 5.59EE157 pKa = 5.23EE158 pKa = 4.78DD159 pKa = 3.79TCCPAYY165 pKa = 9.79IHH167 pKa = 6.62AASNSVAEE175 pKa = 4.53HH176 pKa = 6.24SANAVPPPMEE186 pKa = 4.38GVHH189 pKa = 6.69PGTSPDD195 pKa = 3.64GVPSSSSAVGISLAVDD211 pKa = 3.44VTHH214 pKa = 6.72VNTVDD219 pKa = 4.04ASTEE223 pKa = 3.94MPTKK227 pKa = 10.76VEE229 pKa = 4.29GPDD232 pKa = 3.42EE233 pKa = 4.5GVSTSPLQEE242 pKa = 4.99AGPSMAFVDD251 pKa = 4.29ASSVAMEE258 pKa = 3.81LRR260 pKa = 11.84ARR262 pKa = 11.84FGYY265 pKa = 10.01RR266 pKa = 11.84SKK268 pKa = 11.41NPANLEE274 pKa = 3.82LGGRR278 pKa = 11.84VARR281 pKa = 11.84DD282 pKa = 3.21ILKK285 pKa = 9.97GCGAVRR291 pKa = 11.84SEE293 pKa = 4.02NYY295 pKa = 10.27YY296 pKa = 10.39LAQLAVQLWFAPTLLDD312 pKa = 3.52LVLRR316 pKa = 11.84GPVQDD321 pKa = 4.13FCC323 pKa = 6.41

Molecular weight: 34.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6UQC7|B6UQC7_9TOMB Cell-to-cell movement protein OS=Carrot mottle virus OX=68033 PE=4 SV=1
MM1 pKa = 7.43TFRR4 pKa = 11.84IIYY7 pKa = 9.99DD8 pKa = 4.09GIEE11 pKa = 3.54QDD13 pKa = 3.35SIYY16 pKa = 10.84NRR18 pKa = 11.84RR19 pKa = 11.84THH21 pKa = 5.95QPPARR26 pKa = 11.84RR27 pKa = 11.84NNSGRR32 pKa = 11.84TARR35 pKa = 11.84ARR37 pKa = 11.84AWDD40 pKa = 3.87YY41 pKa = 11.65GPSPWHH47 pKa = 5.91QYY49 pKa = 10.72AYY51 pKa = 9.27PHH53 pKa = 6.54SALPPPAPAYY63 pKa = 10.02FEE65 pKa = 5.01PLFQRR70 pKa = 11.84PLAYY74 pKa = 10.29QEE76 pKa = 4.47DD77 pKa = 4.42RR78 pKa = 11.84GHH80 pKa = 6.03AVYY83 pKa = 10.57RR84 pKa = 11.84EE85 pKa = 4.03ARR87 pKa = 11.84HH88 pKa = 4.98SVRR91 pKa = 11.84SARR94 pKa = 11.84ARR96 pKa = 11.84HH97 pKa = 4.83VAGTSSDD104 pKa = 2.74LGARR108 pKa = 11.84HH109 pKa = 6.08RR110 pKa = 11.84PPRR113 pKa = 11.84SGTDD117 pKa = 2.96RR118 pKa = 11.84DD119 pKa = 4.15GPEE122 pKa = 3.75GVYY125 pKa = 9.9TLVLGTPAGRR135 pKa = 11.84FLSEE139 pKa = 4.36LFHH142 pKa = 7.85SFEE145 pKa = 4.49RR146 pKa = 11.84LSHH149 pKa = 6.18EE150 pKa = 4.61CPSVLQSSNPVRR162 pKa = 11.84GGTTGARR169 pKa = 11.84GEE171 pKa = 4.18RR172 pKa = 11.84LLSLLHH178 pKa = 5.86VAASHH183 pKa = 6.19RR184 pKa = 11.84GEE186 pKa = 4.44GAVLPADD193 pKa = 3.82TTGGCGDD200 pKa = 4.04TTSQHH205 pKa = 6.88PIPTPGLEE213 pKa = 4.11PAHH216 pKa = 6.28AVPTRR221 pKa = 11.84RR222 pKa = 11.84DD223 pKa = 3.22DD224 pKa = 3.63EE225 pKa = 5.17RR226 pKa = 11.84YY227 pKa = 10.07GNHH230 pKa = 6.97DD231 pKa = 3.45QGQSIHH237 pKa = 6.87ALPAPHH243 pKa = 7.14LCGKK247 pKa = 8.63VHH249 pKa = 6.84AVGGPCPTRR258 pKa = 11.84EE259 pKa = 4.13SEE261 pKa = 4.13VKK263 pKa = 10.52YY264 pKa = 10.64RR265 pKa = 3.79

Molecular weight: 29.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1737

265

870

434.3

47.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.002 ± 0.812

1.957 ± 0.327

5.584 ± 0.558

5.527 ± 0.455

3.224 ± 0.654

7.83 ± 0.53

2.879 ± 0.921

3.8 ± 0.591

3.224 ± 1.197

7.599 ± 0.697

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.469

3.109 ± 0.307

7.945 ± 0.75

3.627 ± 0.307

6.908 ± 1.099

7.772 ± 0.298

5.815 ± 0.811

8.52 ± 1.091

1.094 ± 0.13

3.282 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski