Escherichia phage ST20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A220IH85|A0A220IH85_9CAUD Uncharacterized protein OS=Escherichia phage ST20 OX=2569975 PE=4 SV=1
MM1 pKa = 7.41GEE3 pKa = 3.81WVKK6 pKa = 10.83CSEE9 pKa = 4.29RR10 pKa = 11.84MPDD13 pKa = 3.25AEE15 pKa = 4.17VSVLVTDD22 pKa = 4.54GSVVIISSYY31 pKa = 11.65NGFDD35 pKa = 2.8WVDD38 pKa = 3.73YY39 pKa = 10.18YY40 pKa = 9.47WTEE43 pKa = 4.31FATHH47 pKa = 6.66WMPLPEE53 pKa = 4.67PPEE56 pKa = 3.97VV57 pKa = 3.36

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220IH92|A0A220IH92_9CAUD Uncharacterized protein OS=Escherichia phage ST20 OX=2569975 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84EE3 pKa = 3.91AFEE6 pKa = 3.82RR7 pKa = 11.84WAVVEE12 pKa = 3.95GLPVNKK18 pKa = 9.95GSKK21 pKa = 9.82KK22 pKa = 9.54EE23 pKa = 3.94YY24 pKa = 9.84LNVRR28 pKa = 11.84TRR30 pKa = 11.84LAWRR34 pKa = 11.84AWKK37 pKa = 10.53AGVLSVANPRR47 pKa = 3.61

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

13317

42

852

185.0

20.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.754 ± 0.533

1.104 ± 0.129

6.435 ± 0.236

6.954 ± 0.4

3.702 ± 0.152

7.577 ± 0.247

1.569 ± 0.175

5.482 ± 0.185

5.85 ± 0.37

7.509 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.448 ± 0.161

4.265 ± 0.209

3.852 ± 0.209

3.672 ± 0.296

5.317 ± 0.246

5.85 ± 0.34

6.495 ± 0.432

7.096 ± 0.281

1.412 ± 0.126

3.657 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski