Dyadobacter jejuensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Dyadobacter

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A316AQ66|A0A316AQ66_9BACT Tetratricopeptide repeat protein OS=Dyadobacter jejuensis OX=1082580 GN=CLV98_102106 PE=4 SV=1
MM1 pKa = 7.03NQDD4 pKa = 2.86NCFLLGYY11 pKa = 9.75IVRR14 pKa = 11.84THH16 pKa = 5.82GMAGNLVFFLDD27 pKa = 3.51VDD29 pKa = 4.12DD30 pKa = 5.31PSDD33 pKa = 4.16YY34 pKa = 11.37EE35 pKa = 4.18DD36 pKa = 4.73LEE38 pKa = 4.84TVFVEE43 pKa = 4.85LKK45 pKa = 10.93GEE47 pKa = 4.03LVPYY51 pKa = 10.25FIEE54 pKa = 5.11DD55 pKa = 3.83LNLQKK60 pKa = 10.57QSNAIVTFEE69 pKa = 4.71DD70 pKa = 3.11VDD72 pKa = 4.37TIEE75 pKa = 4.48KK76 pKa = 10.29AQALVGASLYY86 pKa = 11.04LPIDD90 pKa = 3.81HH91 pKa = 6.7LQSLDD96 pKa = 3.69DD97 pKa = 3.89DD98 pKa = 3.5QFYY101 pKa = 10.01YY102 pKa = 10.91HH103 pKa = 7.45EE104 pKa = 4.38IKK106 pKa = 10.55GYY108 pKa = 8.7QVSDD112 pKa = 3.63QEE114 pKa = 4.4LGALGVVEE122 pKa = 5.02EE123 pKa = 4.79VYY125 pKa = 11.06SLNGQDD131 pKa = 5.47LINMKK136 pKa = 10.62YY137 pKa = 10.15KK138 pKa = 10.38GAEE141 pKa = 3.95VLIPTANDD149 pKa = 2.88IVLKK153 pKa = 10.45ADD155 pKa = 3.49KK156 pKa = 10.05SQKK159 pKa = 9.95ILFVNLPEE167 pKa = 4.75GLLDD171 pKa = 3.66VYY173 pKa = 11.32LDD175 pKa = 3.83TEE177 pKa = 4.49SEE179 pKa = 4.27NIADD183 pKa = 4.92DD184 pKa = 4.04ADD186 pKa = 3.42

Molecular weight:
20.89 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A316API2|A0A316API2_9BACT 23S rRNA (Cytosine1962-C5)-methyltransferase OS=Dyadobacter jejuensis OX=1082580 GN=CLV98_103417 PE=4 SV=1
MM1 pKa = 7.41GLLSSFHH8 pKa = 6.77CLGMCGPIALALPIHH23 pKa = 6.11GQSQRR28 pKa = 11.84RR29 pKa = 11.84QLLGLFSYY37 pKa = 11.09NFGRR41 pKa = 11.84ALLYY45 pKa = 10.74ACLGALTGTLASALSWIGIFRR66 pKa = 11.84YY67 pKa = 10.13LSITAGILMVAYY79 pKa = 8.83VLWSTRR85 pKa = 11.84FQASLPTPAPIQKK98 pKa = 10.06LLSILKK104 pKa = 9.93KK105 pKa = 10.97AMGSMLQRR113 pKa = 11.84KK114 pKa = 8.51NPMAWFLLGSLNGLVPCGLVYY135 pKa = 10.5LALMSSMATGGPLEE149 pKa = 4.69GATFMLLFGIGTMPMMLAVGFFKK172 pKa = 11.07GLIGPSLRR180 pKa = 11.84TRR182 pKa = 11.84MRR184 pKa = 11.84RR185 pKa = 11.84LVPVMLTIAGIWLIARR201 pKa = 11.84GVLIQYY207 pKa = 7.57PASKK211 pKa = 10.58NVTLPICHH219 pKa = 6.46SPATTLEE226 pKa = 4.18EE227 pKa = 4.24KK228 pKa = 10.77

Molecular weight:
24.58 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4730

0

4730

1682925

26

2639

355.8

39.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.419 ± 0.034

0.742 ± 0.011

5.392 ± 0.025

5.868 ± 0.035

4.659 ± 0.023

7.225 ± 0.033

1.976 ± 0.02

6.604 ± 0.029

6.093 ± 0.034

9.826 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.395 ± 0.018

4.945 ± 0.037

4.174 ± 0.021

4.144 ± 0.02

4.407 ± 0.022

6.632 ± 0.029

5.712 ± 0.036

6.381 ± 0.023

1.333 ± 0.012

4.072 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski