Pseudomonas phage vB_PaM_EPA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pakpunavirus; unclassified Pakpunavirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 171 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EJJ9|A0A4Y6EJJ9_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaM_EPA1 OX=2587493 GN=EPA1_8 PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 5.82DD3 pKa = 4.31YY4 pKa = 11.44FWEE7 pKa = 4.07TGMADD12 pKa = 3.89DD13 pKa = 4.97YY14 pKa = 11.87VMTVQEE20 pKa = 4.16VRR22 pKa = 11.84DD23 pKa = 4.18YY24 pKa = 11.54GSPAYY29 pKa = 10.91SMWVQFWFFVIFDD42 pKa = 4.64LARR45 pKa = 11.84KK46 pKa = 8.04GQQ48 pKa = 3.31

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EKY4|A0A4Y6EKY4_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaM_EPA1 OX=2587493 GN=EPA1_3 PE=4 SV=1
MM1 pKa = 7.15NNKK4 pKa = 9.88YY5 pKa = 9.97KK6 pKa = 11.04DD7 pKa = 3.76NIIAEE12 pKa = 4.38YY13 pKa = 10.51CHH15 pKa = 6.71FDD17 pKa = 2.82GRR19 pKa = 11.84RR20 pKa = 11.84PVLSLAARR28 pKa = 11.84WVHH31 pKa = 5.92HH32 pKa = 6.72DD33 pKa = 3.29SRR35 pKa = 11.84VVKK38 pKa = 10.39LAALFACAGYY48 pKa = 10.0GRR50 pKa = 11.84PYY52 pKa = 10.01KK53 pKa = 10.68DD54 pKa = 4.4CIRR57 pKa = 11.84AYY59 pKa = 9.61KK60 pKa = 10.01AAKK63 pKa = 9.53QRR65 pKa = 11.84EE66 pKa = 4.06KK67 pKa = 11.47DD68 pKa = 3.37MRR70 pKa = 11.84DD71 pKa = 3.14PARR74 pKa = 11.84WKK76 pKa = 10.45LSRR79 pKa = 11.84ATKK82 pKa = 9.42RR83 pKa = 11.84ALRR86 pKa = 11.84ARR88 pKa = 11.84LASKK92 pKa = 10.37KK93 pKa = 10.48KK94 pKa = 9.58PAA96 pKa = 4.06

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

171

0

171

27199

35

904

159.1

17.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.114 ± 0.254

1.371 ± 0.112

6.107 ± 0.154

7.717 ± 0.264

4.18 ± 0.127

6.96 ± 0.203

1.916 ± 0.136

5.5 ± 0.142

6.224 ± 0.255

7.897 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.077 ± 0.107

4.11 ± 0.152

3.614 ± 0.151

3.5 ± 0.114

5.614 ± 0.158

5.721 ± 0.173

5.53 ± 0.217

7.287 ± 0.18

1.772 ± 0.102

3.787 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski