Rhodobacterales bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; unclassified Rhodobacterales

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5345 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D0VEZ9|A0A5D0VEZ9_9RHOB Glyoxalase OS=Rhodobacterales bacterium OX=1948890 GN=FMN50_18305 PE=4 SV=1
MM1 pKa = 7.74ADD3 pKa = 3.53DD4 pKa = 4.8SGMANAGDD12 pKa = 3.93NQTNGAGSGPNGEE25 pKa = 4.33AEE27 pKa = 4.05EE28 pKa = 4.57TIAEE32 pKa = 4.29LFDD35 pKa = 5.76DD36 pKa = 4.35YY37 pKa = 11.74LAGFNDD43 pKa = 3.93FDD45 pKa = 4.57AEE47 pKa = 4.37RR48 pKa = 11.84ICDD51 pKa = 4.0CFALPTTIWQHH62 pKa = 4.79EE63 pKa = 4.4KK64 pKa = 10.43GHH66 pKa = 5.54VFNDD70 pKa = 3.51EE71 pKa = 4.16EE72 pKa = 4.18EE73 pKa = 4.19LLEE76 pKa = 4.33NVEE79 pKa = 4.42ALLAALEE86 pKa = 4.32KK87 pKa = 10.93EE88 pKa = 4.51GVLNSDD94 pKa = 3.74FQVVSSHH101 pKa = 5.45VSSSSALVTLDD112 pKa = 3.38WSQEE116 pKa = 3.98SSDD119 pKa = 3.42GDD121 pKa = 3.83VVFEE125 pKa = 4.52FTCHH129 pKa = 5.04YY130 pKa = 10.86QLIQDD135 pKa = 3.95GSDD138 pKa = 2.64WLIAMIVNEE147 pKa = 4.12

Molecular weight: 16.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D0VG19|A0A5D0VG19_9RHOB Uncharacterized protein OS=Rhodobacterales bacterium OX=1948890 GN=FMN50_16750 PE=4 SV=1
MM1 pKa = 7.65IIYY4 pKa = 9.82YY5 pKa = 10.21GYY7 pKa = 10.1GPAMPMLGAPMRR19 pKa = 11.84GMAMRR24 pKa = 11.84ATAMRR29 pKa = 11.84SPAMRR34 pKa = 11.84GAPMRR39 pKa = 11.84GMAMRR44 pKa = 11.84AASMRR49 pKa = 11.84GAPMRR54 pKa = 11.84GAPMRR59 pKa = 11.84GMAMRR64 pKa = 11.84ATAMRR69 pKa = 11.84SPAMRR74 pKa = 11.84GAPMRR79 pKa = 11.84GAPMRR84 pKa = 11.84GMAMRR89 pKa = 11.84ATAMRR94 pKa = 11.84SPAMRR99 pKa = 11.84GAPMRR104 pKa = 11.84GMAMRR109 pKa = 11.84AASMRR114 pKa = 11.84GAPMRR119 pKa = 11.84GAPMRR124 pKa = 11.84GMAMRR129 pKa = 11.84ATAMRR134 pKa = 11.84SPAMRR139 pKa = 11.84GAPMRR144 pKa = 11.84GAPMRR149 pKa = 11.84GMAMRR154 pKa = 11.84ATAMRR159 pKa = 11.84SPAMRR164 pKa = 11.84GAPMRR169 pKa = 11.84GAPMRR174 pKa = 11.84GMAMRR179 pKa = 11.84AAPMQGGRR187 pKa = 3.86

Molecular weight: 19.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5345

0

5345

1748253

31

5111

327.1

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.272 ± 0.04

0.894 ± 0.011

6.054 ± 0.035

6.197 ± 0.034

3.927 ± 0.023

8.453 ± 0.042

1.948 ± 0.016

5.367 ± 0.023

3.608 ± 0.029

10.157 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.517 ± 0.018

2.805 ± 0.02

4.856 ± 0.028

3.098 ± 0.019

6.428 ± 0.035

6.002 ± 0.028

5.502 ± 0.038

7.355 ± 0.025

1.255 ± 0.013

2.306 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski