Phycisphaera mikurensis (strain NBRC 102666 / KCTC 22515 / FYK2301M01)

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Phycisphaerae; Phycisphaerales; Phycisphaeraceae; Phycisphaera; Phycisphaera mikurensis

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0IDX7|I0IDX7_PHYMF N-acetylmuramoyl-L-alanine amidase domain-containing protein OS=Phycisphaera mikurensis (strain NBRC 102666 / KCTC 22515 / FYK2301M01) OX=1142394 GN=PSMK_13060 PE=4 SV=1
MM1 pKa = 8.0VIEE4 pKa = 5.41DD5 pKa = 3.73FAEE8 pKa = 4.49FNPTTQVVDD17 pKa = 3.63PSFGIFRR24 pKa = 11.84SGVGPAGSGSNGGAVSGTTAISSTPWDD51 pKa = 3.79FTPGGGTSYY60 pKa = 11.53VNNLSITDD68 pKa = 3.84DD69 pKa = 3.66NLFGGGLNYY78 pKa = 10.23GVPTAWTLRR87 pKa = 11.84HH88 pKa = 5.87IANGGAPSAANLVSAAGGVGSVGFYY113 pKa = 10.95LQTTSPDD120 pKa = 3.14LSVRR124 pKa = 11.84IALDD128 pKa = 3.32EE129 pKa = 4.42TGGSGGTEE137 pKa = 3.61GSVAQDD143 pKa = 3.38VIADD147 pKa = 4.25GEE149 pKa = 4.39YY150 pKa = 10.67HH151 pKa = 7.31LYY153 pKa = 9.45QWSLADD159 pKa = 3.55AAQWNPLFGASNGEE173 pKa = 4.04LDD175 pKa = 3.51GTTYY179 pKa = 10.56TIDD182 pKa = 3.59SIVFYY187 pKa = 11.0DD188 pKa = 5.03LGAEE192 pKa = 4.45DD193 pKa = 5.73GSTSDD198 pKa = 4.13FNLAYY203 pKa = 10.36VVADD207 pKa = 3.39NTGPLVNVVPEE218 pKa = 4.2PVGLLALGLAGFGLLGRR235 pKa = 11.84RR236 pKa = 11.84RR237 pKa = 11.84AGG239 pKa = 3.13

Molecular weight: 24.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0IGW5|I0IGW5_PHYMF Beta-glucosidase OS=Phycisphaera mikurensis (strain NBRC 102666 / KCTC 22515 / FYK2301M01) OX=1142394 GN=PSMK_23440 PE=3 SV=1
MM1 pKa = 6.78STHH4 pKa = 4.92YY5 pKa = 9.93PKK7 pKa = 10.36RR8 pKa = 11.84RR9 pKa = 11.84SLIKK13 pKa = 9.9RR14 pKa = 11.84ARR16 pKa = 11.84KK17 pKa = 8.72FGFRR21 pKa = 11.84ARR23 pKa = 11.84MKK25 pKa = 9.71TSLGRR30 pKa = 11.84KK31 pKa = 5.67MLNRR35 pKa = 11.84KK36 pKa = 8.95RR37 pKa = 11.84RR38 pKa = 11.84VGRR41 pKa = 11.84SVNVRR46 pKa = 11.84KK47 pKa = 10.45SFF49 pKa = 3.35

Molecular weight: 5.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3269

0

3269

1143530

24

4118

349.8

37.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.799 ± 0.086

0.893 ± 0.016

6.042 ± 0.035

6.014 ± 0.041

3.261 ± 0.029

9.88 ± 0.039

2.147 ± 0.024

3.035 ± 0.027

2.137 ± 0.034

10.46 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.761 ± 0.019

1.937 ± 0.023

6.415 ± 0.038

2.425 ± 0.023

8.319 ± 0.051

4.72 ± 0.026

4.902 ± 0.034

7.751 ± 0.039

1.403 ± 0.021

1.702 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski