Lactobacillus phage phiJB

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3PCP8|U3PCP8_9CAUD Minor structural protein OS=Lactobacillus phage phiJB OX=1399941 GN=phiJB_00032 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.9FHH4 pKa = 7.49CNWCGTILEE13 pKa = 5.01KK14 pKa = 10.86GDD16 pKa = 4.39SYY18 pKa = 11.88LEE20 pKa = 3.92YY21 pKa = 10.42QGLGFCCMEE30 pKa = 4.64CATDD34 pKa = 4.34YY35 pKa = 11.39IMSDD39 pKa = 3.35VEE41 pKa = 4.22LLEE44 pKa = 4.3KK45 pKa = 10.88VVGEE49 pKa = 4.46DD50 pKa = 3.4EE51 pKa = 4.3RR52 pKa = 11.84LL53 pKa = 3.33

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3PIV2|U3PIV2_9CAUD SLT domain-containing protein OS=Lactobacillus phage phiJB OX=1399941 GN=phiJB_00044 PE=4 SV=1
MM1 pKa = 7.79SDD3 pKa = 3.29LSVFNRR9 pKa = 11.84MAQSTGSRR17 pKa = 11.84NVRR20 pKa = 11.84LQVLNQMHH28 pKa = 6.54QDD30 pKa = 3.28MEE32 pKa = 4.47QYY34 pKa = 9.53VPKK37 pKa = 10.44RR38 pKa = 11.84AGFLRR43 pKa = 11.84SQSFVNDD50 pKa = 3.4TGVHH54 pKa = 4.27YY55 pKa = 7.95TAKK58 pKa = 9.81YY59 pKa = 10.7ARR61 pKa = 11.84AQFYY65 pKa = 10.86GFVNGHH71 pKa = 5.43RR72 pKa = 11.84VRR74 pKa = 11.84NYY76 pKa = 7.21STPGTGRR83 pKa = 11.84RR84 pKa = 11.84WDD86 pKa = 3.7LRR88 pKa = 11.84AKK90 pKa = 10.25AVYY93 pKa = 9.58KK94 pKa = 10.78ADD96 pKa = 3.46WQKK99 pKa = 10.83VAVAAFLKK107 pKa = 9.55EE108 pKa = 4.19AKK110 pKa = 9.95GGYY113 pKa = 8.95

Molecular weight: 12.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11380

53

1179

247.4

27.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.234 ± 0.472

0.764 ± 0.146

7.03 ± 0.32

5.316 ± 0.363

3.866 ± 0.265

7.179 ± 0.523

1.784 ± 0.161

6.019 ± 0.236

7.847 ± 0.463

7.329 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.169

4.93 ± 0.251

3.383 ± 0.277

4.297 ± 0.308

3.954 ± 0.304

6.547 ± 0.29

6.96 ± 0.48

6.441 ± 0.233

1.406 ± 0.1

4.2 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski