Spiribacter salinus M19-40

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Spiribacter; Spiribacter salinus

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4V728|R4V728_9GAMM GMP synthase OS=Spiribacter salinus M19-40 OX=1260251 GN=SPISAL_03580 PE=4 SV=1
MM1 pKa = 7.07TARR4 pKa = 11.84IACRR8 pKa = 11.84CFMLLSLLVPLASVSAEE25 pKa = 4.16DD26 pKa = 5.31ADD28 pKa = 4.28TLYY31 pKa = 11.19LFNWSQYY38 pKa = 8.02MDD40 pKa = 4.07PSIIEE45 pKa = 3.97AFEE48 pKa = 4.46AEE50 pKa = 4.37HH51 pKa = 5.72EE52 pKa = 4.53VEE54 pKa = 4.45VVEE57 pKa = 6.4NYY59 pKa = 10.12FNSNGEE65 pKa = 4.25MFSKK69 pKa = 10.45LQAGGVSQYY78 pKa = 11.69DD79 pKa = 3.32VVVPSNYY86 pKa = 9.53YY87 pKa = 9.21VPRR90 pKa = 11.84LIEE93 pKa = 4.07SDD95 pKa = 3.36LVQRR99 pKa = 11.84LDD101 pKa = 3.27KK102 pKa = 11.25AQIPNLDD109 pKa = 3.43NLMEE113 pKa = 4.22TFIDD117 pKa = 3.89PAYY120 pKa = 10.76DD121 pKa = 3.69PGNDD125 pKa = 3.17YY126 pKa = 11.1SAAYY130 pKa = 8.97QWGTTGLVYY139 pKa = 10.62DD140 pKa = 4.99RR141 pKa = 11.84SALGEE146 pKa = 4.16QPASWSVLFDD156 pKa = 4.26PDD158 pKa = 4.14VNPDD162 pKa = 3.15APFAVPEE169 pKa = 4.74DD170 pKa = 3.97GQVTLGAACAYY181 pKa = 10.0LGHH184 pKa = 7.04GYY186 pKa = 10.67DD187 pKa = 3.72CTDD190 pKa = 3.28RR191 pKa = 11.84DD192 pKa = 4.15ALEE195 pKa = 3.96EE196 pKa = 3.91AARR199 pKa = 11.84LVLDD203 pKa = 4.65AKK205 pKa = 11.02ARR207 pKa = 11.84DD208 pKa = 3.65NFAGFIQGTPVLQQLVRR225 pKa = 11.84GSVSAGMTFNGDD237 pKa = 3.15YY238 pKa = 10.53FYY240 pKa = 11.29LKK242 pKa = 10.71EE243 pKa = 4.1EE244 pKa = 4.44DD245 pKa = 3.89PEE247 pKa = 4.43GFADD251 pKa = 3.69IEE253 pKa = 4.25FVIPEE258 pKa = 4.22EE259 pKa = 4.34GAEE262 pKa = 3.95IWVDD266 pKa = 3.13TMMIPADD273 pKa = 3.76APNPEE278 pKa = 4.39LAHH281 pKa = 6.53AFINFILDD289 pKa = 3.69AEE291 pKa = 4.65VGAQLSNWNYY301 pKa = 9.88YY302 pKa = 10.43SSPNAAALPLLNEE315 pKa = 4.35VLQAPPISPTDD326 pKa = 3.41EE327 pKa = 4.24TMEE330 pKa = 4.25TLAFTPSLMGDD341 pKa = 3.51EE342 pKa = 4.81LQFLQQIWRR351 pKa = 11.84EE352 pKa = 4.13VEE354 pKa = 4.0SRR356 pKa = 3.4

Molecular weight: 39.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4VCN4|R4VCN4_9GAMM Histidine kinase OS=Spiribacter salinus M19-40 OX=1260251 GN=SPISAL_00050 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.5RR12 pKa = 11.84KK13 pKa = 9.44RR14 pKa = 11.84NHH16 pKa = 5.44GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.53GRR39 pKa = 11.84VRR41 pKa = 11.84LTPP44 pKa = 3.82

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1684

0

1684

549060

38

1849

326.0

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.233 ± 0.076

0.834 ± 0.019

6.069 ± 0.058

6.47 ± 0.064

3.205 ± 0.04

8.702 ± 0.056

2.333 ± 0.031

4.732 ± 0.043

1.962 ± 0.04

10.882 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.026

2.373 ± 0.04

5.102 ± 0.05

3.789 ± 0.04

7.743 ± 0.07

4.93 ± 0.032

5.221 ± 0.052

7.492 ± 0.046

1.359 ± 0.027

2.229 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski