Agromyces binzhouensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agromyces

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3584 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2JWA0|A0A4Q2JWA0_9MICO ABC transporter permease OS=Agromyces binzhouensis OX=1817495 GN=ESO86_01300 PE=4 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84RR3 pKa = 11.84MIEE6 pKa = 3.88RR7 pKa = 11.84LRR9 pKa = 11.84GGEE12 pKa = 3.9RR13 pKa = 11.84GASAVLVGLMIVPLIGAAAIAVDD36 pKa = 3.98VGALYY41 pKa = 10.78AEE43 pKa = 5.14RR44 pKa = 11.84AAQQNGADD52 pKa = 3.86GAAMAVATACAADD65 pKa = 4.25EE66 pKa = 4.5SACGASAGSIASDD79 pKa = 3.6FVLQNSFISSPTALAPQIDD98 pKa = 4.21YY99 pKa = 10.54DD100 pKa = 4.2ANRR103 pKa = 11.84VTVTTDD109 pKa = 2.78NSVSHH114 pKa = 6.94PLASVLTGINSTVVQAVSSAEE135 pKa = 3.8WGSPNSGIVLPLALSLCEE153 pKa = 4.13FQDD156 pKa = 3.3PTLEE160 pKa = 3.71IRR162 pKa = 11.84VLIEE166 pKa = 3.31YY167 pKa = 9.34HH168 pKa = 4.74QTSEE172 pKa = 4.04NDD174 pKa = 3.48CTRR177 pKa = 11.84SDD179 pKa = 3.69GQPVEE184 pKa = 5.72GGFGWLDD191 pKa = 3.58LEE193 pKa = 5.05GGQCEE198 pKa = 4.27TFIDD202 pKa = 4.64LDD204 pKa = 3.83TGFVGSDD211 pKa = 3.3PGIDD215 pKa = 3.45PAAEE219 pKa = 3.76CAYY222 pKa = 10.65LFNDD226 pKa = 4.41LEE228 pKa = 4.91GEE230 pKa = 4.51TVLVPVYY237 pKa = 10.88DD238 pKa = 4.03GGNDD242 pKa = 3.09INGQNGEE249 pKa = 4.03FHH251 pKa = 7.23IMAFAAFVVTGWKK264 pKa = 8.41LTGGGPGVFNNPDD277 pKa = 4.16PLSPACVGACRR288 pKa = 11.84GIQGYY293 pKa = 6.83FTEE296 pKa = 4.68WVDD299 pKa = 5.68LGADD303 pKa = 3.73WEE305 pKa = 4.66VGGPDD310 pKa = 3.35FGVDD314 pKa = 3.23VVRR317 pKa = 11.84LVITDD322 pKa = 3.51SQLADD327 pKa = 4.0LLDD330 pKa = 3.7

Molecular weight: 34.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2J4D3|A0A4Q2J4D3_9MICO Uncharacterized protein (Fragment) OS=Agromyces binzhouensis OX=1817495 GN=ESO86_17555 PE=4 SV=1
MM1 pKa = 7.64PGGVAAQAVRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84PRR20 pKa = 11.84RR21 pKa = 11.84PRR23 pKa = 11.84TRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84APRR30 pKa = 11.84CRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84AGTPRR46 pKa = 11.84GRR48 pKa = 11.84CGRR51 pKa = 11.84PASRR55 pKa = 11.84ARR57 pKa = 11.84RR58 pKa = 11.84SPRR61 pKa = 3.05

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3584

0

3584

1048746

32

1954

292.6

31.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.855 ± 0.059

0.47 ± 0.01

6.528 ± 0.034

6.093 ± 0.042

3.136 ± 0.025

9.203 ± 0.037

2.034 ± 0.02

4.33 ± 0.025

1.682 ± 0.033

9.954 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.749 ± 0.016

1.8 ± 0.021

5.453 ± 0.033

2.411 ± 0.024

7.767 ± 0.05

5.247 ± 0.026

5.595 ± 0.028

9.216 ± 0.044

1.528 ± 0.019

1.949 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski