Staphylococcus phage phiRS7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3PG03|U3PG03_9CAUD Uncharacterized protein OS=Staphylococcus phage phiRS7 OX=1403390 GN=phiRS7_003 PE=4 SV=1
MM1 pKa = 8.28DD2 pKa = 4.29YY3 pKa = 10.8KK4 pKa = 11.04SCNKK8 pKa = 9.59VEE10 pKa = 4.38SVRR13 pKa = 11.84VDD15 pKa = 3.06YY16 pKa = 11.11LYY18 pKa = 10.56FYY20 pKa = 10.57EE21 pKa = 5.75VYY23 pKa = 10.44DD24 pKa = 3.62EE25 pKa = 5.3YY26 pKa = 11.58YY27 pKa = 10.02IEE29 pKa = 5.5DD30 pKa = 4.56LDD32 pKa = 5.12NFAQSIDD39 pKa = 3.01HH40 pKa = 7.01HH41 pKa = 6.65MNTNNYY47 pKa = 9.65KK48 pKa = 9.85VFEE51 pKa = 4.22VDD53 pKa = 2.84TLEE56 pKa = 3.97NEE58 pKa = 4.17RR59 pKa = 11.84LIIDD63 pKa = 3.34TTKK66 pKa = 10.26IVNVRR71 pKa = 11.84ILYY74 pKa = 9.69KK75 pKa = 10.7

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3PDW7|U3PDW7_9CAUD Prohead protease OS=Staphylococcus phage phiRS7 OX=1403390 GN=phiRS7_005 PE=4 SV=1
MM1 pKa = 7.18TQDD4 pKa = 3.21QLLEE8 pKa = 3.89QRR10 pKa = 11.84KK11 pKa = 9.54RR12 pKa = 11.84NSKK15 pKa = 10.11RR16 pKa = 11.84YY17 pKa = 7.93NASVRR22 pKa = 11.84YY23 pKa = 9.75GKK25 pKa = 8.31DD26 pKa = 2.65TKK28 pKa = 10.39YY29 pKa = 9.63TEE31 pKa = 5.63FYY33 pKa = 10.56QSSAWRR39 pKa = 11.84SKK41 pKa = 10.29RR42 pKa = 11.84KK43 pKa = 9.34RR44 pKa = 11.84VLLRR48 pKa = 11.84DD49 pKa = 3.33KK50 pKa = 10.58YY51 pKa = 11.21LCQEE55 pKa = 4.28CLKK58 pKa = 10.89QGIVNDD64 pKa = 3.42KK65 pKa = 10.35RR66 pKa = 11.84LMVHH70 pKa = 6.94HH71 pKa = 6.65IVEE74 pKa = 5.25LKK76 pKa = 10.8DD77 pKa = 3.08DD78 pKa = 3.68WKK80 pKa = 11.19RR81 pKa = 11.84RR82 pKa = 11.84LDD84 pKa = 3.74MNNLITVCTACHH96 pKa = 5.51NRR98 pKa = 11.84IEE100 pKa = 4.31HH101 pKa = 5.26TPRR104 pKa = 11.84RR105 pKa = 11.84KK106 pKa = 10.04PEE108 pKa = 3.61VV109 pKa = 3.31

Molecular weight: 13.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13352

34

1810

215.4

24.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.422 ± 0.487

0.569 ± 0.127

6.231 ± 0.457

7.415 ± 0.666

4.291 ± 0.217

6.014 ± 0.591

1.633 ± 0.168

7.347 ± 0.3

8.838 ± 0.357

8.089 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.161

6.786 ± 0.33

2.868 ± 0.236

4.321 ± 0.245

4.067 ± 0.22

6.186 ± 0.241

5.924 ± 0.309

5.992 ± 0.174

1.108 ± 0.174

4.389 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski