Bovine group B rotavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus B

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6KF86|Q6KF86_9REOV Outer capsid glycoprotein VP7 OS=Bovine group B rotavirus OX=35334 GN=VP7 PE=3 SV=1
MM1 pKa = 8.02PLPLLLVLAACAKK14 pKa = 9.3AQLIITPISNPEE26 pKa = 3.61ICVLHH31 pKa = 6.92ANDD34 pKa = 4.38RR35 pKa = 11.84NVNTFGEE42 pKa = 4.27NFTNIFEE49 pKa = 4.87SYY51 pKa = 11.2NSVTLSFYY59 pKa = 11.1NYY61 pKa = 10.31DD62 pKa = 3.57SNNYY66 pKa = 9.78DD67 pKa = 3.8VIDD70 pKa = 3.82ILSKK74 pKa = 10.58KK75 pKa = 10.31DD76 pKa = 3.47YY77 pKa = 10.63SLCQILAIDD86 pKa = 4.34VIRR89 pKa = 11.84PEE91 pKa = 3.68MDD93 pKa = 4.33FITFLQSNNEE103 pKa = 3.75CSKK106 pKa = 10.69YY107 pKa = 10.16SRR109 pKa = 11.84QKK111 pKa = 9.79IHH113 pKa = 5.52YY114 pKa = 8.27QKK116 pKa = 10.11LTTGDD121 pKa = 2.86EE122 pKa = 3.97WFVYY126 pKa = 10.29SKK128 pKa = 11.0NLKK131 pKa = 9.78FCPLSDD137 pKa = 3.76NLIGLYY143 pKa = 10.43CDD145 pKa = 3.46TQVNGTYY152 pKa = 10.25FPLSNNEE159 pKa = 4.16RR160 pKa = 11.84YY161 pKa = 10.04DD162 pKa = 3.63VTDD165 pKa = 3.66LPEE168 pKa = 4.25FTEE171 pKa = 3.96MGYY174 pKa = 10.53VFYY177 pKa = 10.98SDD179 pKa = 4.25DD180 pKa = 4.04DD181 pKa = 4.4FYY183 pKa = 10.89ICKK186 pKa = 10.14RR187 pKa = 11.84VTEE190 pKa = 4.36EE191 pKa = 3.11KK192 pKa = 9.67WINYY196 pKa = 8.41HH197 pKa = 6.33LFYY200 pKa = 10.77RR201 pKa = 11.84EE202 pKa = 3.82YY203 pKa = 10.22SASGTVARR211 pKa = 11.84AISWDD216 pKa = 3.87NVWTGFKK223 pKa = 10.06TFAQVVYY230 pKa = 10.17KK231 pKa = 10.64ILDD234 pKa = 3.15IFFNNRR240 pKa = 11.84RR241 pKa = 11.84NFEE244 pKa = 3.84PRR246 pKa = 11.84AA247 pKa = 3.56

Molecular weight: 28.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6JPH2|Q6JPH2_9REOV Nonstructural protein 5 OS=Bovine group B rotavirus OX=35334 GN=NSP5 PE=4 SV=1
MM1 pKa = 7.53GSSQSSLQTQVHH13 pKa = 5.25STNIHH18 pKa = 5.07SQHH21 pKa = 6.8SSIHH25 pKa = 5.35LQGTSTATFTTHH37 pKa = 6.73QIIITAGAVLIALLLTSLVFSCICNCYY64 pKa = 10.24LYY66 pKa = 11.09SKK68 pKa = 9.9VKK70 pKa = 10.8NGFQTIPLQFKK81 pKa = 9.72RR82 pKa = 11.84KK83 pKa = 8.59EE84 pKa = 4.19RR85 pKa = 11.84FDD87 pKa = 3.71SSIPQPRR94 pKa = 11.84IQPTQFVV101 pKa = 3.57

Molecular weight: 11.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

4698

101

1158

469.8

53.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.13 ± 0.412

1.596 ± 0.571

5.811 ± 0.562

5.449 ± 0.358

4.47 ± 0.473

3.853 ± 0.449

1.724 ± 0.287

7.642 ± 0.314

6.301 ± 0.74

8.365 ± 0.556

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.15

6.003 ± 0.376

4.172 ± 0.349

3.917 ± 0.451

5.194 ± 0.399

8.472 ± 0.38

6.982 ± 0.498

6.599 ± 0.502

1.022 ± 0.158

3.959 ± 0.486
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski