Micrococcus sp. HMSC067E09

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Micrococcus; unclassified Micrococcus

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2073 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1F1IA96|A0A1F1IA96_9MICC Uncharacterized protein OS=Micrococcus sp. HMSC067E09 OX=1739367 GN=HMPREF2863_00660 PE=4 SV=1
MM1 pKa = 7.3VLTTFTVLADD11 pKa = 3.18IAEE14 pKa = 4.32NVAGDD19 pKa = 3.78NLRR22 pKa = 11.84VEE24 pKa = 5.35SITKK28 pKa = 10.02VGAEE32 pKa = 3.49IHH34 pKa = 6.66GYY36 pKa = 10.06EE37 pKa = 4.47PTPGDD42 pKa = 2.99IRR44 pKa = 11.84RR45 pKa = 11.84ASEE48 pKa = 3.91ADD50 pKa = 4.1LILDD54 pKa = 3.31NGMNLEE60 pKa = 3.77AWFAQFVDD68 pKa = 4.27GLDD71 pKa = 3.55VPHH74 pKa = 6.25VVVSEE79 pKa = 4.14GVEE82 pKa = 4.2TIDD85 pKa = 4.62ISEE88 pKa = 4.3DD89 pKa = 3.23AYY91 pKa = 10.88AGMPNPHH98 pKa = 6.13AWMSPVNVQIYY109 pKa = 8.55VDD111 pKa = 3.6NMVEE115 pKa = 4.15AFSDD119 pKa = 4.25LDD121 pKa = 3.71PDD123 pKa = 3.68NASEE127 pKa = 4.28YY128 pKa = 9.11EE129 pKa = 4.33ANGEE133 pKa = 4.03AYY135 pKa = 9.38KK136 pKa = 11.1AEE138 pKa = 4.13LQTVQDD144 pKa = 3.99EE145 pKa = 4.68LVDD148 pKa = 3.89EE149 pKa = 4.99LSVLPEE155 pKa = 3.8NQRR158 pKa = 11.84ALVTCEE164 pKa = 3.73GAFSYY169 pKa = 10.5LARR172 pKa = 11.84DD173 pKa = 3.26AGLTEE178 pKa = 4.16KK179 pKa = 11.08YY180 pKa = 9.07IWAVNAEE187 pKa = 4.24QQATPQQITSAIEE200 pKa = 3.62FVRR203 pKa = 11.84DD204 pKa = 3.13NDD206 pKa = 3.66VPAVFCEE213 pKa = 4.42STVSDD218 pKa = 3.7APMQQVVEE226 pKa = 4.23ATDD229 pKa = 3.29AAFGGVLYY237 pKa = 10.86VDD239 pKa = 4.61SLSEE243 pKa = 4.01ADD245 pKa = 4.55GPVPTYY251 pKa = 11.06LEE253 pKa = 5.06LIRR256 pKa = 11.84HH257 pKa = 6.5DD258 pKa = 3.44ATTIIDD264 pKa = 4.06ALTGGNN270 pKa = 3.63

Molecular weight: 29.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1F1IEY8|A0A1F1IEY8_9MICC Alpha-L-glutamate ligase OS=Micrococcus sp. HMSC067E09 OX=1739367 GN=HMPREF2863_09120 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84KK16 pKa = 8.24HH17 pKa = 4.59GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.21GRR40 pKa = 11.84AALSAA45 pKa = 3.96

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2073

0

2073

705702

40

1807

340.4

36.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.561 ± 0.081

0.6 ± 0.013

6.23 ± 0.042

6.524 ± 0.049

2.794 ± 0.031

9.106 ± 0.046

2.294 ± 0.025

3.459 ± 0.039

2.201 ± 0.047

9.908 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.123 ± 0.02

1.762 ± 0.029

5.454 ± 0.043

3.15 ± 0.029

7.392 ± 0.053

5.165 ± 0.04

5.981 ± 0.033

9.036 ± 0.056

1.457 ± 0.023

1.802 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski