Rhodococcus phage Whack

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Whackvirus; Rhodococcus virus Whack

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A515MKG8|A0A515MKG8_9CAUD Uncharacterized protein OS=Rhodococcus phage Whack OX=2591132 GN=66 PE=4 SV=1
MM1 pKa = 7.74TYY3 pKa = 10.05QHH5 pKa = 7.28LDD7 pKa = 3.25LGVDD11 pKa = 3.95PFSEE15 pKa = 4.53EE16 pKa = 4.57CLPADD21 pKa = 3.95PDD23 pKa = 4.04PFHH26 pKa = 6.87EE27 pKa = 5.2HH28 pKa = 7.44DD29 pKa = 4.62PRR31 pKa = 11.84DD32 pKa = 4.49DD33 pKa = 3.71APEE36 pKa = 3.86WTKK39 pKa = 11.52

Molecular weight: 4.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A515MKA3|A0A515MKA3_9CAUD Uncharacterized protein OS=Rhodococcus phage Whack OX=2591132 GN=42 PE=4 SV=1
MM1 pKa = 7.33TNVLNVADD9 pKa = 3.63NVYY12 pKa = 10.42RR13 pKa = 11.84NGRR16 pKa = 11.84SGVKK20 pKa = 9.37QAMTTDD26 pKa = 5.44PISMHH31 pKa = 6.58LGNLRR36 pKa = 11.84LHH38 pKa = 6.46NSSATTIKK46 pKa = 10.46HH47 pKa = 6.29RR48 pKa = 11.84LDD50 pKa = 3.13NLQRR54 pKa = 11.84IQAKK58 pKa = 9.77LGKK61 pKa = 9.02PLIEE65 pKa = 4.31ATADD69 pKa = 5.21DD70 pKa = 4.16LAAWQANLRR79 pKa = 11.84ISPSSIATYY88 pKa = 8.11TAHH91 pKa = 6.26VRR93 pKa = 11.84AFYY96 pKa = 10.45RR97 pKa = 11.84WAQLNEE103 pKa = 5.07LITVDD108 pKa = 3.47PTVFLVTPKK117 pKa = 10.46LKK119 pKa = 10.41RR120 pKa = 11.84RR121 pKa = 11.84MPRR124 pKa = 11.84PIPEE128 pKa = 4.73RR129 pKa = 11.84DD130 pKa = 3.14LSAALTTSRR139 pKa = 11.84HH140 pKa = 4.7DD141 pKa = 3.51HH142 pKa = 5.77QLFCAFLLAGYY153 pKa = 9.95CGLRR157 pKa = 11.84CGEE160 pKa = 3.97ITRR163 pKa = 11.84VTRR166 pKa = 11.84SDD168 pKa = 3.19VRR170 pKa = 11.84EE171 pKa = 4.21DD172 pKa = 3.63EE173 pKa = 4.32NGKK176 pKa = 9.38GAYY179 pKa = 9.64LVVHH183 pKa = 6.44GKK185 pKa = 10.15GGHH188 pKa = 4.95EE189 pKa = 4.2RR190 pKa = 11.84IIRR193 pKa = 11.84IAPDD197 pKa = 3.27IYY199 pKa = 11.39AEE201 pKa = 4.17IRR203 pKa = 11.84SLMYY207 pKa = 9.38RR208 pKa = 11.84TGPLFVSKK216 pKa = 10.45SGNAYY221 pKa = 8.33TPNRR225 pKa = 11.84LTQITSAHH233 pKa = 6.23LKK235 pKa = 10.69AIGLPYY241 pKa = 9.61TLHH244 pKa = 6.03TLRR247 pKa = 11.84HH248 pKa = 5.65RR249 pKa = 11.84FATRR253 pKa = 11.84LADD256 pKa = 4.0LGADD260 pKa = 3.47VRR262 pKa = 11.84DD263 pKa = 3.71VQAALGHH270 pKa = 6.12AHH272 pKa = 7.81LSTTTLYY279 pKa = 10.75LASNSRR285 pKa = 11.84RR286 pKa = 11.84STSSVDD292 pKa = 2.99RR293 pKa = 11.84LGRR296 pKa = 11.84GLAAMAKK303 pKa = 8.46RR304 pKa = 11.84QPSARR309 pKa = 11.84STSRR313 pKa = 11.84DD314 pKa = 3.26DD315 pKa = 4.67DD316 pKa = 4.0LL317 pKa = 6.92

Molecular weight: 35.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

15671

38

1966

203.5

22.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.41 ± 0.5

0.753 ± 0.12

6.911 ± 0.289

6.12 ± 0.339

2.699 ± 0.166

8.181 ± 0.505

1.997 ± 0.174

4.977 ± 0.217

3.95 ± 0.229

7.913 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.182 ± 0.122

3.152 ± 0.188

5.89 ± 0.278

3.305 ± 0.142

6.63 ± 0.301

6.056 ± 0.209

6.649 ± 0.449

7.287 ± 0.217

1.908 ± 0.149

2.029 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski