Saccharomonospora phage PIS 136

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4AZD6|I4AZD6_9CAUD Uncharacterized protein OS=Saccharomonospora phage PIS 136 OX=182851 GN=PIS_003 PE=4 SV=1
MM1 pKa = 7.8SYY3 pKa = 10.28QGNTGCSCGGGGTSSGGGSGGGTTVVCSRR32 pKa = 11.84PSIPVAVPGMCLSDD46 pKa = 3.48GTPIATISTRR56 pKa = 11.84AKK58 pKa = 9.88DD59 pKa = 3.89GTWVRR64 pKa = 11.84TGWLNLSTGGFTAGAPPASAKK85 pKa = 9.98PCNPSDD91 pKa = 3.65NGGGEE96 pKa = 4.22PATLAGVHH104 pKa = 5.57VEE106 pKa = 4.31DD107 pKa = 4.3WCDD110 pKa = 3.25VDD112 pKa = 5.88DD113 pKa = 5.68DD114 pKa = 5.41GNVLSPVLAKK124 pKa = 11.01YY125 pKa = 7.62EE126 pKa = 4.08TDD128 pKa = 3.51DD129 pKa = 3.25NGAIVGVEE137 pKa = 3.83FLTPDD142 pKa = 3.76GQPYY146 pKa = 9.68EE147 pKa = 4.06IQGTLGICNTDD158 pKa = 3.04GPADD162 pKa = 3.56LTGRR166 pKa = 11.84EE167 pKa = 4.32LVRR170 pKa = 11.84LCDD173 pKa = 3.6VADD176 pKa = 4.97DD177 pKa = 3.89GTITVFVRR185 pKa = 11.84DD186 pKa = 3.97YY187 pKa = 11.09EE188 pKa = 4.28RR189 pKa = 11.84DD190 pKa = 3.36SAGAVVGFTDD200 pKa = 4.52YY201 pKa = 11.28DD202 pKa = 3.44IDD204 pKa = 4.05GQPYY208 pKa = 8.13TPSGEE213 pKa = 4.28VTSCDD218 pKa = 3.07AAQTEE223 pKa = 4.82EE224 pKa = 4.53EE225 pKa = 4.78CASPTTPLATTGLCLADD242 pKa = 3.69GTPIATVSTRR252 pKa = 11.84DD253 pKa = 3.24CDD255 pKa = 4.07GVVSTDD261 pKa = 2.51GWINLLTNTYY271 pKa = 8.76TAGQPPAGARR281 pKa = 11.84ACGEE285 pKa = 3.79TSAFDD290 pKa = 3.67VSGVLCDD297 pKa = 3.73VNEE300 pKa = 4.51SGDD303 pKa = 3.65VLGLVLIEE311 pKa = 4.17VEE313 pKa = 4.32RR314 pKa = 11.84GTDD317 pKa = 3.74GEE319 pKa = 4.37ITGTRR324 pKa = 11.84LINAADD330 pKa = 3.68GSEE333 pKa = 4.03YY334 pKa = 10.48TLQGEE339 pKa = 4.58LTVCPAGVDD348 pKa = 3.44QPEE351 pKa = 3.79RR352 pKa = 11.84DD353 pKa = 4.09LIEE356 pKa = 5.05LCDD359 pKa = 3.9LTDD362 pKa = 4.42DD363 pKa = 5.15GEE365 pKa = 4.76GTTATRR371 pKa = 11.84FLRR374 pKa = 11.84DD375 pKa = 3.16YY376 pKa = 11.53ARR378 pKa = 11.84DD379 pKa = 3.59EE380 pKa = 4.25NGTIVGYY387 pKa = 11.08ADD389 pKa = 3.51YY390 pKa = 9.69TLSGDD395 pKa = 3.78PYY397 pKa = 9.13TPSGNVGSCAAGAAQDD413 pKa = 4.13PQDD416 pKa = 3.59HH417 pKa = 6.56YY418 pKa = 11.43LVQGHH423 pKa = 6.31PMCVVDD429 pKa = 5.23ADD431 pKa = 3.74NAIVRR436 pKa = 11.84RR437 pKa = 11.84VLSEE441 pKa = 3.89HH442 pKa = 6.83VYY444 pKa = 10.86DD445 pKa = 3.58ATTGEE450 pKa = 4.24RR451 pKa = 11.84VEE453 pKa = 4.18TRR455 pKa = 11.84IVDD458 pKa = 3.78PVTGEE463 pKa = 4.04PVDD466 pKa = 4.31LGAGEE471 pKa = 4.45TVADD475 pKa = 4.39CADD478 pKa = 3.42AAADD482 pKa = 5.31DD483 pKa = 4.48EE484 pKa = 4.77PCRR487 pKa = 11.84NTATLMLCDD496 pKa = 4.16VVTDD500 pKa = 4.76DD501 pKa = 5.69SDD503 pKa = 4.61DD504 pKa = 4.48PEE506 pKa = 4.46PTWVQVDD513 pKa = 4.46FEE515 pKa = 4.54QLPTTPPAYY524 pKa = 9.52TGSLPNGVTFTVQGGMPAPRR544 pKa = 11.84GYY546 pKa = 10.43RR547 pKa = 11.84FSNAHH552 pKa = 5.95SNAPDD557 pKa = 4.14GPQRR561 pKa = 11.84WNFDD565 pKa = 3.22QPVMVRR571 pKa = 11.84VGITDD576 pKa = 3.36VQTQLTTNPNGWHH589 pKa = 5.4EE590 pKa = 4.39CVRR593 pKa = 11.84VDD595 pKa = 5.29SKK597 pKa = 11.68LEE599 pKa = 3.83PEE601 pKa = 4.96GVHH604 pKa = 6.9SLHH607 pKa = 6.68EE608 pKa = 3.96WDD610 pKa = 3.32ATNRR614 pKa = 11.84TLCGTADD621 pKa = 3.57TEE623 pKa = 4.65GGGGEE628 pKa = 4.22AVTVFASSEE637 pKa = 4.27PITEE641 pKa = 4.22FEE643 pKa = 5.92LIDD646 pKa = 4.37LTTNPNNQVVGVSTSFVEE664 pKa = 4.37VLLPASGDD672 pKa = 3.72EE673 pKa = 4.25PNVTVTRR680 pKa = 11.84FLRR683 pKa = 11.84HH684 pKa = 5.9IVTDD688 pKa = 4.02CATGEE693 pKa = 4.06IVSINDD699 pKa = 3.27TTLDD703 pKa = 3.73GEE705 pKa = 4.48PYY707 pKa = 9.71EE708 pKa = 4.32VTGEE712 pKa = 4.15VEE714 pKa = 3.98QCQIEE719 pKa = 4.61CGSSCNITPGAPGAPGSPTDD739 pKa = 4.08PQPCQNVTTILLCDD753 pKa = 4.38TIDD756 pKa = 4.89DD757 pKa = 4.23GDD759 pKa = 4.25GEE761 pKa = 4.48QVVPFLRR768 pKa = 11.84SYY770 pKa = 11.1QIDD773 pKa = 3.87NDD775 pKa = 3.76SGTITGHH782 pKa = 6.43TDD784 pKa = 3.1TEE786 pKa = 4.93LDD788 pKa = 3.04GSTPYY793 pKa = 10.49EE794 pKa = 3.92PQGEE798 pKa = 4.41VGQCQPASSGADD810 pKa = 3.42PLPEE814 pKa = 4.64FDD816 pKa = 3.6VAPIVLCDD824 pKa = 3.59TVDD827 pKa = 3.46GGEE830 pKa = 4.32GEE832 pKa = 4.23QAVEE836 pKa = 3.79FLRR839 pKa = 11.84FFRR842 pKa = 11.84VDD844 pKa = 3.01NSTGEE849 pKa = 4.46VTGHH853 pKa = 6.67TDD855 pKa = 3.25TEE857 pKa = 4.66LDD859 pKa = 4.05GITPYY864 pKa = 10.65EE865 pKa = 3.98PQGEE869 pKa = 4.39VGQCQSGGEE878 pKa = 4.25CCEE881 pKa = 4.1PPEE884 pKa = 4.59PVPSLDD890 pKa = 3.23IRR892 pKa = 11.84CEE894 pKa = 4.1TVVLCDD900 pKa = 3.88TGPDD904 pKa = 3.48GDD906 pKa = 4.85DD907 pKa = 3.17PVSFMRR913 pKa = 11.84AICRR917 pKa = 11.84DD918 pKa = 2.88SSGNVVSVTDD928 pKa = 3.76TEE930 pKa = 4.53LDD932 pKa = 3.15AVTPYY937 pKa = 9.9TVRR940 pKa = 11.84SS941 pKa = 3.62

Molecular weight: 98.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4AZE8|I4AZE8_9CAUD ExsB OS=Saccharomonospora phage PIS 136 OX=182851 GN=thiL PE=4 SV=1
MM1 pKa = 7.53SRR3 pKa = 11.84RR4 pKa = 11.84DD5 pKa = 3.4PHH7 pKa = 6.15TEE9 pKa = 3.55LLALEE14 pKa = 4.4LEE16 pKa = 4.26RR17 pKa = 11.84RR18 pKa = 11.84GYY20 pKa = 9.96RR21 pKa = 11.84IRR23 pKa = 11.84WRR25 pKa = 11.84HH26 pKa = 4.48NWCFTATRR34 pKa = 11.84NGRR37 pKa = 11.84SEE39 pKa = 4.69VFWSTRR45 pKa = 11.84SSLNSQIASQVAKK58 pKa = 10.53RR59 pKa = 11.84KK60 pKa = 9.71DD61 pKa = 3.01ICSRR65 pKa = 11.84LLTDD69 pKa = 4.77AGVSAPTEE77 pKa = 3.87RR78 pKa = 11.84LFRR81 pKa = 11.84ASSASAAAEE90 pKa = 4.04WASGRR95 pKa = 11.84WPVVVKK101 pKa = 9.81PATSPGHH108 pKa = 5.05STGATVGVTTADD120 pKa = 3.51GVRR123 pKa = 11.84TAVAKK128 pKa = 10.08AARR131 pKa = 11.84VSSLVLVQQQVRR143 pKa = 11.84GSEE146 pKa = 3.58ARR148 pKa = 11.84MLVVGGRR155 pKa = 11.84CVHH158 pKa = 6.07VLRR161 pKa = 11.84KK162 pKa = 9.61LGKK165 pKa = 8.01PGAYY169 pKa = 9.18EE170 pKa = 4.87AITEE174 pKa = 4.28RR175 pKa = 11.84VHH177 pKa = 6.97PSYY180 pKa = 11.08LRR182 pKa = 11.84AAEE185 pKa = 4.08RR186 pKa = 11.84AVAAIPGLGLAGLDD200 pKa = 3.72VIAEE204 pKa = 4.24DD205 pKa = 3.09WHH207 pKa = 7.63KK208 pKa = 9.9PGKK211 pKa = 9.68YY212 pKa = 9.9AVIEE216 pKa = 4.34VNSAPGIKK224 pKa = 9.59GHH226 pKa = 6.5HH227 pKa = 6.7RR228 pKa = 11.84PTKK231 pKa = 10.54GRR233 pKa = 11.84GFDD236 pKa = 3.22AAGAIVDD243 pKa = 3.69EE244 pKa = 4.76LEE246 pKa = 3.94RR247 pKa = 11.84RR248 pKa = 11.84TT249 pKa = 3.76

Molecular weight: 26.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

132

0

132

28532

34

1238

216.2

23.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.138 ± 0.419

1.535 ± 0.162

6.575 ± 0.241

5.822 ± 0.232

2.324 ± 0.122

8.065 ± 0.281

2.87 ± 0.184

3.491 ± 0.139

2.555 ± 0.197

8.363 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.138

2.699 ± 0.138

6.046 ± 0.229

3.603 ± 0.184

8.825 ± 0.44

5.66 ± 0.345

6.677 ± 0.259

8.086 ± 0.275

1.77 ± 0.111

1.787 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski