Rubrivivax gelatinosus (strain NBRC 100245 / IL144)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Rubrivivax; Rubrivivax gelatinosus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0HXF0|I0HXF0_RUBGI MaoC family dehydratase-like protein OS=Rubrivivax gelatinosus (strain NBRC 100245 / IL144) OX=983917 GN=RGE_43510 PE=4 SV=1
MM1 pKa = 8.01LDD3 pKa = 3.38PRR5 pKa = 11.84LPRR8 pKa = 11.84YY9 pKa = 9.03QWGQEE14 pKa = 3.54VLAAVDD20 pKa = 4.91LYY22 pKa = 11.64NDD24 pKa = 3.64GSVPEE29 pKa = 4.4AEE31 pKa = 4.39EE32 pKa = 4.25DD33 pKa = 3.31QLLIVQGGPGEE44 pKa = 4.13IVQVGHH50 pKa = 6.44HH51 pKa = 6.76AEE53 pKa = 4.27ANVPLYY59 pKa = 10.21MVDD62 pKa = 5.26FGLCVLGCLEE72 pKa = 4.23EE73 pKa = 4.81EE74 pKa = 4.28IVPVTGAEE82 pKa = 3.96AGGG85 pKa = 3.48

Molecular weight: 9.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0HW85|I0HW85_RUBGI Histidine kinase OS=Rubrivivax gelatinosus (strain NBRC 100245 / IL144) OX=983917 GN=cheA PE=4 SV=1
MM1 pKa = 7.97PILRR5 pKa = 11.84TTVLVLAALLTAGLATMGVAVLAQPLAPRR34 pKa = 11.84LLGVAGQPWFLPTAMALVGLVAVALVVSRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84SRR67 pKa = 11.84RR68 pKa = 3.26

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4686

0

4686

1556812

28

3813

332.2

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.682 ± 0.065

0.893 ± 0.012

5.325 ± 0.023

5.591 ± 0.034

3.233 ± 0.02

8.591 ± 0.042

2.161 ± 0.018

3.531 ± 0.025

2.429 ± 0.03

11.155 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.063 ± 0.017

2.031 ± 0.025

5.575 ± 0.033

3.494 ± 0.021

8.164 ± 0.044

4.78 ± 0.039

4.867 ± 0.039

7.903 ± 0.028

1.55 ± 0.016

1.981 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski