Microbacterium sp. C448

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3165 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0Z7B5|W0Z7B5_9MICO Putative Cystathionine beta-synthase OS=Microbacterium sp. C448 OX=1177594 GN=MIC448_1240001 PE=4 SV=1
MM1 pKa = 7.19MATAGKK7 pKa = 7.6RR8 pKa = 11.84TNWFAIWVSVAVVAVVVVAGVAVVLINNAASNPDD42 pKa = 3.95PGPAPEE48 pKa = 3.83AAIVNTEE55 pKa = 3.76TGAISVGDD63 pKa = 4.01GPDD66 pKa = 3.25VVATYY71 pKa = 10.49IDD73 pKa = 4.59FMCPICNQFEE83 pKa = 4.47GIYY86 pKa = 10.77AEE88 pKa = 4.21TLNDD92 pKa = 3.84LVADD96 pKa = 4.47GSITLEE102 pKa = 3.92YY103 pKa = 10.42HH104 pKa = 6.13PVAILDD110 pKa = 3.83NQSQGTQFSTRR121 pKa = 11.84AANALYY127 pKa = 10.19CVATTTPEE135 pKa = 3.6AVTPFVSAMYY145 pKa = 10.56ANQPSEE151 pKa = 4.08GTTGLSDD158 pKa = 4.85DD159 pKa = 5.05EE160 pKa = 5.13IISIATDD167 pKa = 3.23AGAADD172 pKa = 4.07AASCITDD179 pKa = 3.23RR180 pKa = 11.84TYY182 pKa = 11.21EE183 pKa = 4.08GFVGEE188 pKa = 4.66RR189 pKa = 11.84TQAMPTTADD198 pKa = 3.51GGRR201 pKa = 11.84GTPTVTFNGEE211 pKa = 4.2RR212 pKa = 11.84IDD214 pKa = 3.96LTGDD218 pKa = 3.48PQVDD222 pKa = 3.59IIDD225 pKa = 3.96RR226 pKa = 11.84LSS228 pKa = 3.02

Molecular weight: 23.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0ZCQ8|W0ZCQ8_9MICO GRAM_POS_ANCHORING domain-containing protein OS=Microbacterium sp. C448 OX=1177594 GN=MIC448_40017 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.87GRR40 pKa = 11.84TTVSAA45 pKa = 4.02

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3165

0

3165

972437

20

2545

307.2

32.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.249 ± 0.064

0.493 ± 0.01

6.311 ± 0.039

5.768 ± 0.041

3.102 ± 0.028

8.783 ± 0.035

1.993 ± 0.021

4.788 ± 0.032

1.887 ± 0.033

10.019 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.846 ± 0.018

1.955 ± 0.02

5.478 ± 0.032

2.687 ± 0.023

7.625 ± 0.051

5.75 ± 0.029

5.959 ± 0.034

8.836 ± 0.042

1.514 ± 0.019

1.959 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski